广西师范大学学报（自然科学版） ›› 2025, Vol. 43 ›› Issue (4): 157-164.doi: 10.16088/j.issn.1001-6600.2024041401

• 生态环境科学研究 • 上一篇    下一篇

氧化石墨烯介导的荧光强度和荧光偏振同时检测农药啶虫脒

尹南竹1,2, 黄茜1,2, 赵晶瑾1,2*   

  1. 1.广西生态脆弱区环境过程与修复重点实验室(广西师范大学), 广西 桂林 541006;
    2.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西 桂林 541006
  • 收稿日期:2024-04-14 修回日期:2024-06-27 出版日期:2025-07-05 发布日期:2025-07-14
  • 通讯作者: 赵晶瑾(1984—),女,湖南慈利人,广西师范大学教授,博士。E-mail: jzhao12@163.com
  • 基金资助:
    国家自然科学基金(22376040,21966009)

Fluorescence Intensity and Polarization Method for Acetamiprid Detection Based on Graphene Oxide

YIN Nanzu1,2, HUANG Qian1,2, ZHAO Jingjin1,2*   

  1. 1. Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), Guilin Guangxi 541006, China;
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China
  • Received:2024-04-14 Revised:2024-06-27 Online:2025-07-05 Published:2025-07-14

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摘要： 本研究基于氧化石墨烯(GO)的荧光猝灭效应和质量放大效应,建立一种荧光强度和荧光偏振双信号同时检测方法,用于农药啶虫脒的定量分析。标记有荧光分子的核酸信号探针吸附在GO表面时,检测到较低的荧光强度和较高的偏振信号。当啶虫脒与其适配体结合后,释放的互补核酸探针与信号探针杂交而使其远离石墨烯,此时得到增强的荧光强度和降低的偏振信号。实验考察不同浓度啶虫脒的荧光强度和偏振信号之间的关系,并优化目标物识别时间、氧化石墨烯的浓度和反应时间等条件。实验结果表明:在优化的实验条件下,荧光偏振法(LOD=5 nmol/L)比荧光强度(LOD=50 nmol/L)的检出限更低,而荧光强度法更稳定。该方法具有较好的选择性,并实现对芹菜叶和湖水中啶虫脒的分析。

关键词: 啶虫脒, 氧化石墨烯, 荧光分析, 荧光偏振, 荧光猝灭

Abstract: A fluorescence intensity and polarization method for simultaneous detection of pesticide acetamiprid was established based on the fluorescence quenching effect and mass amplification effect of graphene oxide (GO). When nucleic acid signal probe which labeled with fluorescent molecule FAM attached to the GO surface, a lower fluorescence intensity and a higher polarization signal was detected. After acetamiprid binds to its aptamer, the released complementary probe was hybridized with the signal probe to move it away from graphene, and then the enhanced fluorescence intensity and the reduced polarization signal are obtained. The relationship between the fluorescence intensity or polarization signal and different concentrations of acetamiprid was examined, and the conditions such as the target recognition time, GO concentration and reaction time were optimized. Under the optimized experimental conditions, the fluorescence polarization detection method (LOD=5 nmol/L) had a lower detection limit than the fluorescence intensity method (LOD=50 nmol/L), while the fluorescence intensity method was more stable. This method showed good selectivity and achieved spiked recovery analysis of acetamiprid in celery leaves and lake water.

Key words: acetamiprid, graphene oxide, fluorescence analysis, fluorescence polarization, fluorescence quenching

中图分类号:  O657.3;X839.2;TQ450.263

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