广西师范大学学报（自然科学版） ›› 2018, Vol. 36 ›› Issue (2): 148-156.doi: 10.16088/j.issn.1001-6600.2018.02.021

• • 上一篇    

HO-1介导耐力运动抗NAFLD形成作用的研究

刘波1,许思毛2*   

  1. 1.广西师范大学漓江学院,广西桂林 541006;
    2. 广西师范大学体育学院,广西桂林 541006
  • 出版日期:2018-05-10 发布日期:2018-07-18
  • 通讯作者: 许思毛(1978—),男,安徽芜湖人,广西师范大学副教授。E-mail:xusimao666@163.com
  • 基金资助:
    广西自然科学基金(2013GXNSFBA019146)

Effect of Endurance Exercise on HO-1 Expression and Its Resistance to Non-Alcoholic Fatty Liver Disease Formation

LIU Bo1,XU Simao2*   

  1. 1. Lijiang College, Guangxi Normal University,Guilin Guangxi 541006, China;
    2.College of Physical Education, Guangxi Normal University,Guilin Guangxi 541006,China
  • Online:2018-05-10 Published:2018-07-18

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摘要： 为探讨中等强度耐力运动对大鼠肝脏血红素加氧酶-1(HO-1)蛋白表达的调节作用,及其与抗高脂饲料大鼠非酒精性脂肪肝(NAFLD)形成作用的关系,将48只雄性SD大鼠,随机分为普通饲料对照组(NC)、运动普通饲料组(EN)、抑制剂+运动普通饲料组(DEN)及高脂饲料对照组(HC)、运动高脂饲料组(EH)、抑制剂+运动高脂饲料组(DEH)。观察血清TC、TG、FFC浓度及肝脏HO-1蛋白表达与活性、肝脏抗氧化能力与MDA含量的变化,以及血转氨酶ALT、AST水平及肝脏形态学变化,结果发现:NC、EN、DEN这3组中,EN组肝脏HO-1蛋白表达、活性及肝脏总抗氧化能力最高(P<0.05)。与 NC 组相比,HC、EH、DEH组血清TC、TG、FFC浓度均显著升高,肝脏HO-1蛋白表达均显著升高,肝脏HO-1活性、抗氧化能力均显著下降,MDA含量均显著升高,血清ALT、AST水平均显著升高及肝脏呈典型脂肪样病变;其中EH组的肝脏HO-1蛋白表达最高,但上述异常变化程度最轻(P<0.05)。此外,EH组肝脏HO-1蛋白表达也要显著高于EN组(P<0.05)。可见高脂膳食引发大鼠血脂异常,肝脏HO-1活性及肝脏抗氧化能力下降、活性氧水平升高,形成脂肪肝。中等强度耐力运动可通过诱导肝脏HO-1的蛋白表达,提高高脂饲料大鼠肝脏HO-1活性及抗氧化能力,清除活性氧,发挥抗脂肪肝形成的作用。

关键词: 中等强度耐力运动, 血红素加氧酶-1, 非酒精性脂肪肝, 抗氧化

Abstract: To explore the relationship of moderate intensity endurance exercise on Heme Oxygenase-1 (HO-1) protein expression in liver and its resistance to Non-Alcoholic Fatty Liver Disease Formation of rats fed with high-fat diet, 48 male SD rats were randomly divided into normal diet control group (NC), exercise and normal diet group (EN), the inhibitor + exercise and normal diet group (DEN) and high fat diet control group (HC), exercise and high fat diet group (EH), the inhibitor + exercise and high fat diet group (DEH). The changes of blood TC, TG, FFC concentrations and the expression ＆ the activity of HO-1 protein in the liver were observed, as well as for the changes of Total Antioxidant Capacity ＆ MDA content and the changes of blood ALT, AST concentrations ＆ liver morphology. The results showed that, in the groups of NC, EN and DEN, the expression and activity of HO-1 protein ＆ the Total Antioxidant Capacity of liver in group EN were the highest. Compared with group NC, groups HC, EH and DEH showed significantly higher concentrations of blood TC, TG, FFC, and a much higher expression of HO-1 protein with a significantly decreased HO-1 activity and a Total Antioxidant Capacity in the liver, as well as much higher MDA content in the liver and serum ALT, AST levels which suggested typical liver fatty degeneration. Among them, the liver HO-1 protein expression of group EH was the highest, but the above-mentioned abnormal changes for group EH were the lightest. In addition, the expression of HO-1 protein in the liver of group EH was significantly higher than that of group EN. Some conclusions could be drawn from these studies. High fat diet easily induced dyslipidemia in rats, decreased HO-1 activity and antioxidant capacity of liver, and increased reactive oxygen species. Moderate intensity endurance exercise can induce the expression of HO-1 protein in liver, increase the HO-1 activity in the livers of rats fed with high fat diet and the ability of antioxidation to eliminate reactive oxygen species, preventing NAFLD formation and protecting liver function.

Key words: moderately intense endurance exercise, heme oxygenase-1, NAFLD, antioxidation

中图分类号: 

  • G804.2
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