翘嘴鳜USP7基因cDNA的克隆及饥饿对其表达影响分析

doi:10.16088/j.issn.1001-6600.2018.02.020

广西师范大学学报（自然科学版） ›› 2018, Vol. 36 ›› Issue (2): 141-147.doi: 10.16088/j.issn.1001-6600.2018.02.020

翘嘴鳜USP7基因cDNA的克隆及饥饿对其表达影响分析

张方亮¹,邓小红²,朱晓彤¹,徐蕾¹,胡姜丽¹,宾石玉^1*

1.广西师范大学生命科学学院,广西桂林 541006;
2. 全州县水产技术推广站,广西桂林 541500

收稿日期:2017-05-10 出版日期:2018-05-10 发布日期:2018-07-18
通讯作者: 宾石玉(1963—), 男, 广西全州人, 广西师范大学教授, 博士。E-mail:binsy@gxnu.edu.cn
基金资助:
国家自然科学基金(31560718)

Cloning and Expression Analysis of Ubiquitin-Specific Protease 7 Gene under Starvation in Mandarin Fish (Siniperca chuatsi)

ZHANG Fangliang¹,DENG Xiaohong²,ZHU Xiaotong¹,XU Lei¹,HU Jiangli¹,BIN Shiyu^1*

1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006,China;
2.Quanzhou Fisheries Technology Station, Guilin Guangxi 541500,China

Received:2017-05-10 Online:2018-05-10 Published:2018-07-18

摘要/Abstract

摘要： 为探讨饥饿对翘嘴鳜肝脏USP7基因表达的影响,本文应用实时荧光定量PCR、3′-RACE和5′-RACE技术,从翘嘴鳜肝脏组织中克隆得到了USP7基因的全长cDNA序列(登录号:MF000683)为4 319 bp。生物信息学分析表明:翘嘴鳜USP7基因编码区全长为3 336 bp,共编码个1 111氨基酸,其中180个带负电荷,137个带正电荷。翘嘴鳜USP7在饥饿中的表达分析表明,肝脏USP7基因在不同饥饿时间的相对表达量呈现一个峰值变化,在饥饿4 d时出现显著的上升且到达最高值;在饥饿7 d时又逐渐下降,最终达到初始水平(P<0.05)。本文结果发现饥饿后肝脏中USP7表达量的变化可能与翘嘴鳜的生长和健康状况密切相关,为鳜鱼的生产养殖提供一定的理论依据。

关键词: 翘嘴鳜, 泛素特异性蛋白酶7基因(USP7), 肝脏, 表达分析

Abstract: To investigate the effect of starvation on the expression of USP7 gene in the liver of Mandarin fish, the full-length cDNA sequence of the USP7 gene was cloned from the liver tissue of Mandarin fish by 3′-RACE and 5′-RACE techniques (Accession No. MF000683) with 4 319 bp and its expression profile upon starvation was assayed by real-time quantitative PCR. Bioinformatics analysis showed that the full-length coding region of USP7 gene is 3 336 bp with 1 111 encoded amino acids. Among them, 180 and 137 ones are negatively and positively charged, respectively. The relative expression of USP7 gene in the liver at different starvation time showed a peak change, which had a significant increase and reached the highest value after a starvation duration of 4 days, then decreased gradually after a starvation duration of 7 days and finally reached the initial level (P<0.05). Our studies provided novel information about the USP7gene and its expression status upon starvation.

Key words: Mandarin fish(Siniperca chuatsi), USP7 gene, liver, expression analysis

中图分类号:

Q959.483

张方亮, 邓小红, 朱晓彤, 徐蕾, 胡姜丽, 宾石玉. 翘嘴鳜USP7基因cDNA的克隆及饥饿对其表达影响分析[J]. 广西师范大学学报（自然科学版）, 2018, 36(2): 141-147.

ZHANG Fangliang, DENG Xiaohong, ZHU Xiaotong, XU Lei, HU Jiangli, BIN Shiyu. Cloning and Expression Analysis of Ubiquitin-Specific Protease 7 Gene under Starvation in Mandarin Fish (Siniperca chuatsi)[J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(2): 141-147.

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翘嘴鳜USP7基因cDNA的克隆及饥饿对其表达影响分析

Cloning and Expression Analysis of Ubiquitin-Specific Protease 7 Gene under Starvation in Mandarin Fish (Siniperca chuatsi)

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