广西师范大学学报（自然科学版） ›› 2017, Vol. 35 ›› Issue (1): 92-97.doi: 10.16088/j.issn.1001-6600.2017.01.015

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翘嘴鳜UCP3基因cDNA的克隆及其胚胎发育表达分析

徐蕾1,陈小保2,唐春3,胡姜丽1,宾石玉1,张方亮1   

  1. 1.广西师范大学生命科学学院,广西桂林541006;
    2.宜春职业技术学院食用菌研究所,江西宜春336000;
    3.桂林市水产技术推广站,广西桂林541004
  • 出版日期:2017-01-20 发布日期:2018-07-17
  • 通讯作者: 宾石玉(1963—),男,广西桂林人,广西师范大学教授,博士。E-mail:binsy@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金(31560718)

Cloning and Embryonic Development Expression Analysis of the Uncoupling Proteins 3 Gene in Mandarin Fish

XU Lei1,CHEN Xiaobao2,TANG Chun3,HU Jiangli1,BIN Shiyu1,ZHANG Fangliang1   

  1. 1.College of Life Science, Guangxi Normal University, Guilin Guangxi 541006,China;
    2. Institute of Edible Fungus, Yichun Vocational and Technical College, Yichun Jiangxi 336000, China;
    3.Guilin Fisheries Technology Station, Guilin Guangxi 541004,China
  • Online:2017-01-20 Published:2018-07-17

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摘要： 本研究采用RT-PCR、3′-RACE和5′-RACE技术,从翘嘴鳜胚胎组织中克隆得到UCP3基因的全长cDNA序列(登录号:KP244309)为2 917 bp。生物信息学分析表明:翘嘴鳜UCP3基因编码区为930 bp,总共编码309个氨基酸;带负电荷的氨基酸残基数22个,带正电荷的氨基酸残基数35个。翘嘴鳜UCP3发育表达分析表明,UCP3在受精期、二细胞期、囊胚期、肌效期、心搏期、仔鱼期有低量表达,从囊胚期到神经胚期显著性增高并达最大值,神经胚期到肌效期显著性降低,肌效期、心搏期、仔鱼期都保持在低水平表达。

关键词: 翘嘴鳜;UCP3基因; 克隆;胚胎发育;表达分析

Abstract: The Uncoupling proteins 3 is members of the mitochondrial anion carrier family, which may play important roles in thermogenesis, lipid metabolism and insulin secretion. The physiological regulation of the UCP3 remains practically vague in fish. It is little known for the roles of UCP3 in fish, the UCP3 was cloned from Siniperca chuatsi by Homologous cloning technology. The UCP3 consisted of six exons and one to two exon which could not be translated, and primarily expressed in muscle. The length of UCP3 gene was 2 917 bp and the length of its putative amino acid sequence was 309 residues. To investigate the gene Ontogenetic expression, a real-time PCR technique is applied to assay the ontogenetic expression of the genes at different stages, and the results confirmed that the UCP3 mRNA were first detected in fertilization stage, its expression gradually increased in gastrula stage, and reached the peak of expression in neurula stage.

Key words: Mandarin fish(Siniperca chuatsi), UCP3 gene, cloning, embryonic development, expression analysis

中图分类号: 

  • Q959.483
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