广西师范大学学报（自然科学版） ›› 2024, Vol. 42 ›› Issue (1): 191-199.doi: 10.16088/j.issn.1001-6600.2022121501

• 研究论文 • 上一篇    

发酵罗汉果渣对黄羽肉鸡肠道形态和菌群的影响

孙涛1,2, 梁丽芬1, 许艺兰3, 陈静1, 叶泉清1, 袁梦婷1, 宾石玉1,2*   

  1. 1.广西师范大学 生命科学学院,广西 桂林 541006;
    2.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学),广西 桂林 541006;
    3.广西兽医生物技术重点实验室(广西壮族自治区兽医研究所),广西 南宁 530001
  • 收稿日期:2022-12-15 修回日期:2023-01-26 出版日期:2024-01-25 发布日期:2024-01-19
  • 通讯作者: 宾石玉(1963—),男,广西全州人,广西师范大学教授,博士。E-mail:binsy@gxnu.edu.cn
  • 基金资助:
    广西科技计划项目(桂科AB19259012,桂科AB22035039);广西高等教育本科教学改革工程项目(2022JGZ113)

Effects of Fermented Siraitia grosvenorii Residue on Intestinal Morphology and Microflora of Yellow-Feathered Broilers

SUN Tao1,2, LIANG Lifen1, XU Yilan3, CHEN Jing1, YE Quanqing1, YUAN Mengting1, BIN Shiyu1,2*   

  1. 1. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmented Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    3. Guangxi Key Laboratory of Veterinary Biotechnology (Guangxi Veterinary Research Institute), Nanning Guangxi 530001, China
  • Received:2022-12-15 Revised:2023-01-26 Online:2024-01-25 Published:2024-01-19

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摘要： 本文旨在研究发酵罗汉果渣对黄羽肉鸡肠道形态和菌群的影响。选用1日龄健康的黄羽肉鸡360羽,随机分为4个组(Ⅰ、Ⅱ、Ⅲ和Ⅳ),每组6个重复,每重复15羽。对照组(Ⅰ组)饲喂基础饲粮,Ⅱ、Ⅲ和Ⅳ组于1~21日龄分别饲喂在基础饲粮中添加1%、3%和5%的发酵罗汉果渣等量替代玉米;于22~42日龄和43~70日龄分别在基础饲粮中添加5%、8%和10%的发酵罗汉果渣等量替代玉米,试验期70 d。结果显示:与Ⅰ组相比,21日龄和42日龄,Ⅱ、Ⅲ和Ⅳ组十二指肠、空肠、回肠的绒毛高度、隐窝深度及V/C值的差异不显著。70日龄,Ⅲ、Ⅳ组十二指肠隐窝深度分别降低36.01%(P<0.05)和33.46%(P<0.05),V/C值分别提高78.05%(P<0.05)和69.98%(P<0.05)。肠道菌群中,Ⅰ、Ⅲ组共有的OTU数目为537、特有OTU数目分别为55和7;在门水平上,核心菌门是厚壁菌门、拟杆菌门和变形菌门,其中Ⅲ组的变形菌门和肠杆菌门相对丰度显著低于Ⅰ组(P<0.05);在属水平上,Ⅲ组梭状芽孢杆菌群的相对丰度显著高于Ⅰ组(P<0.05),两组的α多样性指数和β多样性指数的差异不显著(P>0.05)。发酵罗汉果渣可改善黄羽肉鸡肠道微生态环境,促进其肠道健康。

关键词: 罗汉果, 发酵饲料, 肉鸡, 肠道形态, 物种多样性

Abstract: This study was aimed to investigate the effects of fermented Siraitia grosvenorii residue on the intestinal morphology and flora of yellow-feathered broilers. 360 one day old healthy yellow-feathered broilers were randomly divided into 4 groups with 6 replicates in each group and 15 broilers per replicate; Broilersin the control Group (Ⅰ)were fed a basal diet, and the others in (Ⅱ,Ⅲ,Ⅳ) group, 1-21 days old were fed with 1%, 3% and 5% fermented siraitia pomace in the basic diet to replace corn; 22-42 days and 43-70 days old were fed with 5%, 8% and 10% fermented siraitia pomace, respectively. The trial period was 70 days. The results showed that compared with the control Group I, the age of 21 days and 42 days,there were no significant difference between Group in the villus height, recess depth and V/C of duodenum, jejunum and ileum in Ⅰ, Ⅲ and Ⅳ. At 70 days of age, the duodenal recess depth of Group III and Group Ⅳ decreased by 36.01% (P<0.05) and 33.46% (P<0.05), the V/C increased by 78.05% (P<0.05) and 69.98% (P<0.05), respectively. In terms of intestinal flora, the number of OTUs shared by Group I and Group Ⅲ were 537, and the number of unique OTUs in Group I and Group Ⅲ were 55 and 7 respectively; At the phyla level, the core microflora are Firmicutes, Bacteroides and Proteus, among which, the relative abundance of Proteus and Epsilon bacteraeotain Group Ⅲ were significantly lower than that in Group I (P<0.05); At the genus level, the uncultured_bacterium_f_Clostridialgroup in Group III was significantly higher than that of trial Group I (P<0.05); Alpha diversity index and Beta diversity index of Group I and Group III were not significantly different (P>0.05). Fermented Siraitia grosvenorii residue could ameliorate intestinal micro environment and health of Yellow-Feathered Broilers.

Key words: Siraitia grosvenorii, fermented feed, broilers chickens, intestinal morphology, species diversity

中图分类号:  S834

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