广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (3): 155-162.doi: 10.16088/j.issn.1001-6600.2022042901

• 研究论文 • 上一篇    下一篇

肺癌小鼠肿瘤组织IL-10的来源及其功能验证

何柳1,2,3, 蔺超1,2,3, 李培华1,2,3, 陈治秀1,2,3, 朱宇1,2,3, 蒲仕明1,2,3, 周祖平1,2,3*   

  1. 1.广西师范大学 生命科学学院, 广西 桂林 541006;
    2.广西高校干细胞与医药生物技术重点实验室(广西师范大学), 广西 桂林 541004;
    3.广西师范大学 生物医药研究中心, 广西 桂林 541004
  • 收稿日期:2022-04-29 修回日期:2022-06-06 出版日期:2023-05-25 发布日期:2023-06-01
  • 通讯作者: 周祖平(1965—), 男, 广西桂林人, 广西师范大学教授, 博士。E-mail: 192455010@qq.com
  • 基金资助:
    国家自然科学基金(81972700, 61827919); 广西自然科学基金(2018GXNSFBA)

Origin and Functional Verification of IL-10 in Tumor Tissue of Lung Cancer Mice

HE Liu1,2,3, LIN Chao1,2,3, LI Peihua1,2,3, CHENG Zhixiu1,2,3, ZHU Yu1,2,3, PU Shiming1,2,3, ZHOU Zuping1,2,3*   

  1. 1. School of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Guangxi University Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541004, China;
    3. Research Center for Biomedical Sciences, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2022-04-29 Revised:2022-06-06 Online:2023-05-25 Published:2023-06-01

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摘要： 为了解肺癌肿瘤组织白细胞介素-10(interleukin-10,IL-10)的表达和来源,并探究IL-10对肿瘤的作用,采用MSD 超敏电化学发光技术检测Lewis 肺癌小鼠模型血清中IL-10的水平,使用流式细胞术分析肿瘤组织IL-10的主要来源,再通过CCK-8实验、膜联蛋白V-碘化丙啶(annexin V-PI)凋亡实验、划痕实验,检测IL-10重组蛋白对肺癌细胞增殖、凋亡、迁移的影响。结果表明:Lewis肺癌小鼠血清中IL-10水平较正常小鼠显著升高,肿瘤组织中的IL-10主要由免疫细胞产生。其中,B细胞和髓源性抑制细胞(myeloid-derived suppressor cells,MDSCs)是IL-10的主要来源,IL-10重组蛋白可以显著抑制LLC(小鼠 Lewis 肺癌细胞:Lewis lung carcinoma line)的增殖和迁移并促进其凋亡。综上可见,IL-10水平与肺癌密切相关,B细胞和MDSCs是产生IL-10的主要来源,IL-10可能通过激活CD8 T细胞或者直接作用于肿瘤细胞而发挥抗肿瘤作用。

关键词: 肺癌, IL-10, 细胞来源, 功能

Abstract: To investigate the expression and source of interleukin-10 (IL-10) in lung cancer tumor tissues, and to investigate the effect of IL-10 on tumors. The levels of IL-10 in the serum of Lewis lung carcinoma mouse model were detected by MSD hypersensitive electrochemiluminescence assay, the main source of IL-10 in tumor tissues was analyzed using flow cytometry, and the effects of IL-10 recombinant protein on proliferation, apoptosis and migration of lung cancer cells were examined by CCK-8 assay, annexin V-propidium iodide (annexin V-PI) apoptosis assay and scratch wound assay. The results showed that the serum levels of IL-10 were significantly increased in Lewis lung carcinoma mice compared with normal mice, and IL-10 in tumor tissues was mainly produced by immune cells, including B cells and myeloid-derived suppressor cells (MDSCs), which were the main source of IL-10, and the recombinant protein of IL-10 could significantly inhibit the proliferation and migration and promote the apoptosis of LLC (mouse Lewis lung carcinoma line). IL-10 level was closely related to lung cancer, B cells and MDSCs were the main source of IL-10 production, and IL-10 may play an antitumor role by activating CD8 T cells or directly acting on tumor cells.

Key words: lung cancer, IL-10, cell origin, function

中图分类号:  R734.2;Q28

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