广西师范大学学报（自然科学版） ›› 2018, Vol. 36 ›› Issue (4): 124-130.doi: 10.16088/j.issn.1001-6600.2018.04.016

• • 上一篇    下一篇

L-苏氨酸镁对多巴胺神经元的保护作用及其机制

罗娆珊1, 沈艳玲2,3, 孙立元1*   

  1. 1.广西脑与认知神经科学重点实验室,广西桂林541004;
    2.桂林医学院附属医院病理科,广西桂林541004;
    3.厦门大学附属成功医院,福建厦门361000
  • 收稿日期:2017-12-04 发布日期:2018-10-20
  • 通讯作者: 孙立元(1972—),男,吉林延边人,桂林医学院教授,博士,硕导。E-mail: sunly@glmc.edu.cn
  • 基金资助:
    国家自然科学基金(81260205);广西自然科学基金(2015GXNSFAA139137)

Protective Effect of Magnesium-L-Threonateon Dopamine Neurons and Its Mechanism

LUO Raoshan1, SHEN Yanling2,3, SUN Liyuan1*   

  1. 1.Guangxi Key Laboratory of Brain and Cognitive Neuroscience,Guilin Guangxi 541004,China;
    2.Department of Pathology, Affiliated Hospital of Guilin Medical University, Guilin Guangxi 541004, China;
    3.Successful Affiliated Hospital of Xiamen University,Xiamen Fujian 361000, China
  • Received:2017-12-04 Published:2018-10-20

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摘要： 为观察L-苏氨酸镁(MgT)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)小鼠模型的神经保护作用,探讨其可能的机制,本文建立PD小鼠模型,将小鼠随机分为对照组、MPTP组、MPTP+MgT组,采用旋转棒法观察小鼠行为变化,TH染色法观察黑质多巴胺神经元数目变化,蛋白质免疫印迹法检测黑质和纹状体胶质纤维酸性蛋白(GFAP)、诱导型一氧化氮合酶(iNOS)和酪氨酸羟化酶(TH)的表达。结果表明:MPTP+MgT组在旋转轴上停留的时间比MPTP组延长68.07%;MPTP+MgT组黑质多巴胺神经元数目比MPTP组增加46.15%;MPTP+MgT组黑质和纹状体GFAP、iNOS、TH的表达量与MPTP组比较,差异有统计学意义(P<0.05)。可见MgT神经保护作用的机制可能与下调GFAP、iNOS表达,以及抑制炎症、氧化应激有关。

关键词: 帕金森病, L-苏氨酸镁, 酪氨酸羟化酶, 胶质纤维酸性蛋白, 诱导型一氧化氮合酶

Abstract: To evaluate the neuroprotective effect of Magnesium-L-threonate (MgT) on mice model of Parkinson’s disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) and to investigate the possible mechanism.The PD mice model was established. The mice were randomly divided into control group, MPTP group and the MPTP+MgT group. Rotary bar method was used to observe behavioral changes in mice. The number of dopaminergic neurons in substantia nigra was observed by TH staining. The expression of GFAP, iNOS and TH in substantia nigra and striatum were detected by Western blotting.The time for the MPTP+MgT group to stay on the rotating rod was 68.07% longer than that in the MPTP group. The results showed that, in MPTP+MgT group, the number of dopamine neurons in the substantia nigra increased by 46.15% than that in the MPTP group. Compared with MPTP group, the expression of GFAP and iNOS in MPTP+MgT group was statistically significant (P<0.05).The mechanism of MgT neuroprotective effect is probably related to the down-regulation of GFAP and iNOS expression,and inhibition of inflammation and oxidative stress.

Key words: Parkinson’s disease, Magnesium-L-threonate, tyrosine hydroxylase, glial fibrillary acidic protein, inducible nitric oxide synthase

中图分类号: 

  • R742.5
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