一株产天然蓝色素细菌的分离鉴定

doi:10.16088/j.issn.1001-6600.2018.04.017

广西师范大学学报（自然科学版） ›› 2018, Vol. 36 ›› Issue (4): 131-138.doi: 10.16088/j.issn.1001-6600.2018.04.017

一株产天然蓝色素细菌的分离鉴定

肖咪云^1,2, 阮楚晋^1,2,3, 陈寿昆^1,2, 刘裕华^1,2, 陆祖军^1,2*

1.广西师范大学生命科学学院,广西桂林541006;
2.珍稀濒危动植物生态与环境保护重点实验室(广西师范大学),广西桂林541006;
3.中国科学院微生物研究所,北京100101

收稿日期:2017-09-14 发布日期:2018-10-20
通讯作者: 陆祖军(1963—),男(壮族),广西百色人,广西师范大学教授,博士。E-mail:1946208364@qq.com
基金资助:
国家自然科学基金(31060120);广西研究生教育创新计划项目(XYCSZ2017070)

Isolation and Identification of a BacteriumProducing Natural Blue Pigment

XIAO Miyun^1,2, RUAN Chujin^1,2,3, CHEN Shoukun^1,2, LIU Yuhua^1,2, LU Zujun^1,2*

1.College of Life Science, Guangxi Normal University, Guilin Guangxi, 541006,China;
2. Key Laboratory of Ecological and Environmental Protection for Rare and Endangered Animalsand Plants (Guangxi Normal University), Guilin Guangxi 541006,China;
3.Institute of Microbiology,Chinese Academy of Sciences, Beijing 100101,China

Received:2017-09-14 Published:2018-10-20

摘要/Abstract

摘要： 为了分离鉴定一株产天然蓝色素的细菌,本文以表面消毒法从牛大力Millettia specisoa Champ植物根中分离可产天然蓝色素的细菌,以通用引物27F/1492R对目标细菌的16S rDNA序列进行PCR扩增、序列对比分析、构建系统发育树,同时对目标细菌进行形态学观察,并采用GEN III MicroStation 微生物自动鉴定系统对目标细菌进行Biolog鉴定。序列比对结果表明,目标细菌与Pseudomonas aeruginosa相近,相似率为99%;系统发育树显示,其与菌株Pseudomonas aeruginosa在同一条分支上;形态学鉴定为杆状革兰氏阴性;Biolog鉴定目标细菌为铜绿假单胞菌Pseudomonas aeruginosa。综合形态学、分子、Biolog鉴定结果,鉴定该细菌为铜绿假单胞菌,命名为2016NX1。

关键词: 天然蓝色素, 16S rDNA, Biolog鉴定

Abstract: In order to isolate and identify a bacterium producing natural blue pigment, surface sterilization method was applied to isolate producing natural blue pigment target strain from the root of Bovine Millettia specisoa Champ. 16S rDNA sequence of the target strain was amplified by PCR with universal primer 27F/1492R, and the phylogenetic tree was constructed by comparing and analyzing the sequence. The morphology of the bacterium was observed at the same time. The target strain was identified by the GEN III MicroStation microorganism automatic identification system. The results of the sequence alignment showed that the similarity rate of target strain and Pseudomonas aeruginosa was 99% and the phylogenetic tree showed that the target strain was on the same branch of the P. aeruginosa. Morphological identification revealed that it was rod-shaped and Gram negative, and Biolog identification showed that it was P. aeruginosa. The target strain was identified as P. aeruginosa by the combined results of molecular, morphology and Biolog identification and named as 2016NX1.

Key words: natural blue pigment, 16S rDNA, Biolog identification

中图分类号:

Q939

肖咪云, 阮楚晋, 陈寿昆, 刘裕华, 陆祖军. 一株产天然蓝色素细菌的分离鉴定[J]. 广西师范大学学报（自然科学版）, 2018, 36(4): 131-138.

XIAO Miyun, RUAN Chujin, CHEN Shoukun, LIU Yuhua, LU Zujun. Isolation and Identification of a BacteriumProducing Natural Blue Pigment[J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(4): 131-138.

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一株产天然蓝色素细菌的分离鉴定

Isolation and Identification of a BacteriumProducing Natural Blue Pigment

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