岩黄连总生物碱提取工艺及抗氧化活性研究

doi:10.16088/j.issn.1001-6600.2021093003

广西师范大学学报（自然科学版） ›› 2022, Vol. 40 ›› Issue (6): 145-153.doi: 10.16088/j.issn.1001-6600.2021093003

岩黄连总生物碱提取工艺及抗氧化活性研究

李伶^1,2, 唐文迪^1,2, 陈寿涣^1,2, 宁裕龙^1,2, 覃江克^1,2*

1.广西师范大学化学与药学学院,广西桂林541004;
2.省部共建药用资源化学与药物分子工程国家重点实验室(广西师范大学),广西桂林541004

收稿日期:2021-09-30 修回日期:2022-01-20 出版日期:2022-11-25 发布日期:2023-01-17
通讯作者: 覃江克(1977—),男(壮族),广西大化人,广西师范大学教授,博导。E-mail:jiangkeq@sina.com
基金资助:
国家自然科学基金(21462007);广西创新驱动发展项目(桂科AA18118015-4)

Study on Extraction Technology and Antioxidant Activity of Total Alkaloids from Corydalis saxicola Bunting

LI Ling^1,2, TANG Wendi^1,2, CHEN Shouhuan^1,2, NING Yulong^1,2, QIN Jiangke^1,2*

1. School of Chemistry and Pharmaceutical Sciences,Guangxi Normal University, Guilin Guangxi 541004, China;
2. State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Guilin Guangxi 541004, China

Received:2021-09-30 Revised:2022-01-20 Online:2022-11-25 Published:2023-01-17

摘要/Abstract

摘要： 以脱氢卡维丁得率为评价指标,采用单因素实验及正交试验确定岩黄连中总生物碱的最佳提取工艺,并采用自由基清除法评价其抗氧化活性。实验结果表明,岩黄连总生物碱的最优提取工艺为:提取温度80 ℃,提取时间2.5 h,乙醇体积分数60%,料液比1∶14,提取3次,在此条件下得率为7.34%;抗氧化活性实验表明,岩黄连生物碱粗提物对DPPH自由基、超氧阴离子自由基有较好的清除作用,对ABTS自由基和羟基自由基有一定程度的清除作用,呈现浓度依赖性,表明岩黄连生物碱粗提物是潜在的天然抗氧化剂。

关键词: 岩黄连, 生物碱, 提取工艺, 抗氧化活性

Abstract: The extraction process of total alkaloids from Corydalis saxicola Bunting was studied by single factor test and orthogonal test, using the yield of dehydrocavidine as the evaluation index. The antioxidant activity of total alkaloids of C. saxicola Bunting was evaluated by free radical scavenging method in vitro. The results showed that the optimal extraction conditions of total alkaloids were as follows: extraction temperature 80 ℃, extraction time 2.5 h, ethanol concentration 60%, solid-liquid ratio 1∶14, extraction for 3 times, under this condition the total alkaloids yield of C. saxicola Bunting was 7.34%. The antioxidant experiments showed that the crude extract of total alkaloids from C. saxicola Bunting had good scavenging effect on DPPH· and superoxide radical, and had a certain scavenging effect on ABTS⁺· and hydroxyl radical, showing in a concentration dependent manner, whick revealed that the crude extract of total alkaloids from C. saxicola Bunting are potential natural antioxidants.

Key words: Corydalis saxicola Bunting, alkaloid, extraction process, antioxidant activity

中图分类号:

R284.2

李伶, 唐文迪, 陈寿涣, 宁裕龙, 覃江克. 岩黄连总生物碱提取工艺及抗氧化活性研究[J]. 广西师范大学学报（自然科学版）, 2022, 40(6): 145-153.

LI Ling, TANG Wendi, CHEN Shouhuan, NING Yulong, QIN Jiangke. Study on Extraction Technology and Antioxidant Activity of Total Alkaloids from Corydalis saxicola Bunting[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(6): 145-153.

参考文献

[1] 韦莹,李翠,白隆华,等.生长抑制剂对岩黄连种质离体保存的影响[J].时珍国医国药,2021,32(9): 2262-2265.
[2] 张占江, 黄浩, 郭晓云, 等. 瑶药岩黄连研究概况[J]. 植物学研究,2020, 9(4): 424-430.
[3] KUAI C P,JU L J,HU P P,et al.Corydalis saxicola alkaloids attenuate cisplatin-induced neuropathic pain by reducing loss of IENF and blocking TRPV1 activation[J]. The American Journal of Chinese Medicine, 2020,48(2):407-428.
[4] 余姣娇,邱志霞,刘秋燕,等.岩黄连的研究进展[J]. 药学研究,2018,37(6):342-345, 360.
[5] 孟春梅,陆世银,梁永红,等.岩黄连总碱诱导肝星状细胞凋亡和自噬的电镜实验研究[J]. 广西师范大学学报(自然科学版),2018,36(3):76-79.
[6] LI M M,WANG J Y,MO B W,et al.Total alkaloids of Corydalis saxicola bunting inhibits migration of A549 cells by suppressing Cdc42 or Vav1[J]. Oncology Letters,2018,15(1): 475-482.
[7] 吴颖瑞,马云宝,赵友兴,等.岩黄连的抗乙肝病毒活性成分研究[J].中草药,2012,43(1): 32-37.
[8] 安然,王绩英.岩黄连抗肿瘤及其他药理作用的研究进展[J].医学综述,2014,20(11): 1984-1986.
[9] 张成,姚兴东,雷福厚.岩黄连中生物碱的研究进展[J].化工技术与开发,2020,49(12): 9-13, 18.
[10] 唐超玲,刘平,郑华,等.岩黄连化学成分及药理作用研究进展[J].中药新药与临床药理,2018,29(1): 104-109.
[11] 刘春华,胡营,张占江,等.岩黄连的显微鉴别及生物碱的组织化学定位[J].中药材,2019,42(1): 71-73.
[12] 何志超,王冬梅,李国成,等.岩黄连生物碱类成分及其抗氧化活性研究[J].中草药,2014,45(11): 1526-1531.
[13] HUANG Q Q,BI J L,SUN Q Y,et al.Bioactive isoquinoline alkaloids from Corydalis saxicola[J]. Planta Medica,2012,78(1): 65-70.
[14] 王奇志,梁敬钰,原悦.岩黄连化学成分[J].中国天然药物,2007,5(1): 31-34.
[15] LIU X W,TANG C L,ZHENG H,et al.Investigation of the hepatoprotective effect of Corydalis saxicola Bunting on carbon tetrachloride-induced liver fibrosis in rats by ¹H-NMR-based metabonomics and network pharmacology approaches[J]. Journal of Pharmaceutical and Biomedical Analysis,2018,159: 252-261.
[16] 陈萍,鞠霖杰,成俊,等.岩黄连总碱对代谢相关脂肪性肝病小鼠的治疗作用及分子机制研究[J].药物评价研究,2021,44(3): 468-477.
[17] 刘馨烛,邱剑楠,戚莉,等.岩黄连对急性肝内胆汁淤积大鼠的干预作用及胆汁酸转运体表达的影响[J].中华中医药杂志,2019,34(4): 1700-1703.
[18] 李赞忠,李发旺,乔子荣.生物碱提取分离方法研究新进展[J].化工进展,2010,29(S2): 293-299.
[19] 吴杲,陆兔林,毛春芹,等.岩黄连生物碱提取工艺研究[J].中国药师,2010,13(1): 50-51.
[20] 石宝明,迟子涵.自由基对动物的危害及消除技术研究进展[J].饲料工业,2021,42(9): 1-6.
[21] 黄小凤,刘泽干,雷攀,等.柴胡不同极性部位的体外抗氧化活性研究[J].中南药学,2021,19(2): 203-208.
[22] SRIVASTAVA S, SINGH D, PATEL S,et al.Role of enzymatic free radical scavengers in management of oxidative stress in autoimmune disorders[J]. International Journal of Biological Macromolecules,2017,101: 502-507.
[23] GÁRATE-RASCÓN M, RECALDE M, JIMENEZ M,et al.SLU7 prevents oxidative stress-mediated HNF4α degradation preserving hepatic differentiation and protecting from liver damage[J]. Hepatology,2021,74(5): 2791-2807.
[24] REUNGOAT E, GRIGOROV B, ZOULIM F, et al.Molecular crosstalk between the hepatitis C virus and the extracellular matrix in liver fibrogenesis and early carcinogenesis[J]. Cancers,2021,13(9): 2270.
[25] GALLEGO P, LUQUE-SIERRA A, FALCON G,et al. White button mushroom extracts modulate hepatic fibrosis progression, inflammation, and oxidative stress in vitro and in LDLR-/-mice[J]. Foods,2021,10(8): 1788.
[26] 陆世银,郑华,程邦,等.基于组效关系的壮药岩黄连抑制HSC-T6细胞增殖活性成分辨识研究[J].中草药,2017,48(7): 1354-1361.
[27] 杨琳,杨志学.地蚕化学成分的预实验[J].中国民族医药杂志,2007,13(2): 51-52.
[28] 张慧杰,任晓亮,孙浩,等.基于半定量分析方法的药对配伍对制首乌成分含量及DPPH自由基清除能力影响研究[J].中草药,2021,52(7): 1924-1936.
[29] 付露,沙合尼西·赛力克江,洪吟秋,等.地胆草抗氧化活性成分分离鉴定[J].中国实验方剂学杂志,2019,25(2): 156-162.
[30] 郑婷婷,严亮,张文杰,等.水碱连续提取黄皮疣柄牛肝菌粗多糖的理化性质及抗氧化活性研究[J].食品工业科技,2020,41(15): 84-89.
[31] 戴宏杰,孙玉林,郑小林,等.拟目乌贼生殖腺多糖提取工艺优化及自由基清除能力研究[J]. 食品工业科技,2015,36(5):198-203.

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岩黄连总生物碱提取工艺及抗氧化活性研究

Study on Extraction Technology and Antioxidant Activity of Total Alkaloids from Corydalis saxicola Bunting

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