南方红豆杉提取物的抗氧化、抗肿瘤活性研究

doi:10.16088/j.issn.1001-6600.2016.04.009

广西师范大学学报（自然科学版） ›› 2016, Vol. 34 ›› Issue (4): 55-59.doi: 10.16088/j.issn.1001-6600.2016.04.009

• 广西高校优秀中青年骨干教师培养工程论坛 • 上一篇下一篇

南方红豆杉提取物的抗氧化、抗肿瘤活性研究

刘茜¹,张业²

1. 广西师范大学化学与药学学院,广西桂林541004;
2. 桂林师范高等专科学校化学与药学系,广西桂林541001

收稿日期:2016-04-28 出版日期:2016-07-18 发布日期:2018-07-18
通讯作者: 张业(1981—),男(壮族),广西贵港人,桂林师范高等专科学校副教授,博士。E-mail:zhangye81@126.com
基金资助:
国家自然科学基金资助项目(21501032);广西自然科学基金资助项目(2014GXNSFBA118050,2016JJA120113);广西高等学校优秀中青年骨干教师培养工程资助项目(桂教办[2013]258号);广西高校中青年教师基础能力提升资助项目(KY2016YB441);梧州学院重点科研项目(2014B011);广西植物功能物质研究与利用重点实验室开放基金资助项目(FPRU2013-6)

Antioxidant and Antitumor Activities of Extracts from Taxus chinensis var. mairei

LIU Xi¹, ZHANG Ye²

1. College of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin Guangxi 541004, China;
2. Department of Chemistry and Pharmaceutical Science, Guilin Normal College, Guilin Guangxi 541001, China

Received:2016-04-28 Online:2016-07-18 Published:2018-07-18

摘要/Abstract

摘要： 在超声波辅助和80 ℃下,以无水乙醇为溶剂对南方红豆杉进行提取,浓缩后得提取物EE,EE通过柱色谱法分离制得紫杉醇,并对EE和紫杉醇的抗氧化、抗肿瘤活性进行研究。结果表明,EE对DPPH·、ABTS⁺·、O^-₂·、OH· 的清除活性显著优于常用抗氧化剂BHT和紫杉醇,半清除浓度IC₅₀分别为13.19±1.50、2.65±0.35、25.33±1.75和343.00±2.13 mg/L,说明EE具有优良的抗氧化活性;紫杉醇对人非小肺癌细胞A549、人乳腺癌细胞MCF-7、人肝癌细胞Bel-7402和子宫颈癌细胞Hela的抑制活性远优于EE,半抑制浓度IC₅₀分别为3.45±1.37、6.20±0.88、1.98±0.49和1.15±0.27 mg/L。

关键词: 南方红豆杉, 紫杉醇, 抗氧化活性, 抗肿瘤活性, 超声波提取

Abstract: Under ultrasound, Taxus chinensis var. mairei were extracted with ethanol at 80 ℃ to offer ethanol extract (EE). EE was further extracted with chloroform and separated by column chromatography to offer taxol. Their antioxidant and antitumor activities were then evaluated. The antioxidant activity screening results showed that EE exhibited better scavenging activity than the common antioxidant BHT in these four assays, with IC₅₀ of 13.19±1.50, 2.65±0.35, 25.33±1.75 and 343.00±2.13 mg/L, respectively, while taxol showed the worst, indicating excellent antioxidant activity of EE. The antitumor activity screening results demonstrated that taxol showed better inhibition than EE, with IC₅₀ of 3.45±1.37, 6.20±0.88, 1.98±0.49 and 1.15±0.27 mg/L, respectively.

Key words: Taxus chinensis var. mairei, taxol, antioxidant activity, antitumor activity, ultrasound-assisted extract

中图分类号:

R282

刘茜. 南方红豆杉提取物的抗氧化、抗肿瘤活性研究[J]. 广西师范大学学报（自然科学版）, 2016, 34(4): 55-59.

LIU Xi. Antioxidant and Antitumor Activities of Extracts from Taxus chinensis var. mairei[J]. Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(4): 55-59.

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南方红豆杉提取物的抗氧化、抗肿瘤活性研究

Antioxidant and Antitumor Activities of Extracts from Taxus chinensis var. mairei

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