广西师范大学学报（自然科学版） ›› 2025, Vol. 43 ›› Issue (3): 193-200.doi: 10.16088/j.issn.1001-6600.2024050503

• 分子生物学与生物技术 • 上一篇    下一篇

青鳉elavl2基因的表达分析

郭振华, 李新闻, 陈娟, 余世琦, 李晨杰, 关桂君*   

  1. 上海海洋大学水产与生命学院,上海 201306
  • 收稿日期:2024-05-05 修回日期:2024-05-31 出版日期:2025-05-05 发布日期:2025-05-14
  • 通讯作者: 关桂君(1965—),女,浙江镇海人,上海海洋大学教授,博士。E-mail: gjguan@shou.edu.cn
  • 基金资助:
    国家重点研发计划(2018YFD0900601)

Expression Analysis of elavl2 Gene in Medaka

GUO Zhenhua, LI Xinwen, CHEN Juan, YU Shiqi, LI Chenjie, GUAN Guijun*   

  1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
  • Received:2024-05-05 Revised:2024-05-31 Online:2025-05-05 Published:2025-05-14

PDF (PC)

12

摘要： 青鳉gsdf缺失导致生殖细胞过度增殖以及XY生殖细胞性逆转为卵母细胞。转录组比对分析结果显示,gsdf缺失性腺的elavl2基因表达均显著高于正常对照组,提示Gsdf信号可能抑制性腺elavl2的表达。Elavl2在物种间具有较高的进化保守性。elavl2在青鳉胚胎发育和性成熟后各组织中均有表达,在性腺和脑中高表达,提示Elavl2对配子生成和脑的发育有重要作用。化学原位杂交显示,elavl2主要在正常精巢和卵巢中的初级精母细胞和卵母细胞中表达,与qPCR在性腺中相对表达量一致。elavl2在配子生成过程中可能发挥重要功能。本研究为进一步探索脊椎动物性别分化调控机制提供比较进化方面的线索。

关键词: 青鳉, gsdf, elavl2, 配子生成, 性别分化

Abstract: Loss of gsdf in the medaka leads to excessive proliferation of germ cells and XY male germ cell sex reversal to oocytes. Transcriptomic analysis revealed a significant upregulation of the elavl2 gene expression in both XX and XY gsdf-deficient gonads, suggesting a putative inhibition of sex gland elavl2 expression by Gsdf signaling. Elavl2 had a high evolutionary conservation among species. ELAVL2 was expressed in various tissues after embryonic development and sexual maturation, with notably high expression in the gonads and brain, indicated its critical role in gametogenesis and brain function. Chemical in situ hybridization demonstrates predominant expression of elavl2 in primary spermatogonia and oocytes within normal testes and ovaries. Consistent with the relative expression of qPCR in gonads, elavl2 may play an important role in gamete production. this work supports and provides comparative evolutionary insights into the regulatory mechanisms underline the vertebrate sex differentiation.

Key words: medaka, gsdf, elavl2, gamete generation, sex differentiation

中图分类号:  S917.4; Q786

[1] HUNT P A, HASSOLD T J. Sex matters in meiosis[J]. Science, 2002, 296(5576): 2181-2183. DOI: 10.1126/science.1071907.
[2]NISHIMURA T, SATO T, YAMAMOTO Y, et al. foxl3 is a germ cell-intrinsic factor involved in sperm-egg fate decision in medaka[J]. Science, 2015, 349(6245): 328-331. DOI: 10.1126/science.aaa2657.
[3]LI X, LI X W, LI W H, et al. Sex-specific meiosis responses to Gsdf in medaka (Oryzias latipes)[J]. The FEBs Journal, 2023, 290(10): 2760-2779. DOI: 10.1111/febs.16701.
[4]HERPIN A, ADOLFI M C, NICOL B, et al. Divergent expression regulation of gonad development genes in medaka shows incomplete conservation of the downstream regulatory network of vertebrate sex determination[J]. Molecular Biology and Evolution, 2013, 30(10): 2328-2346. DOI: 10.1093/molbev/mst130.
[5]MATSUDA M, NAGAHAMA Y, SHINOMIYA A, et al. DMY is a Y-specific DM-domain gene required for male development in the medaka fish[J]. Nature, 2002, 417(6888): 559-563. DOI: 10.1038/nature751.
[6]NANDA I, KONDO M, HORNUNG U, et al. A duplicated copy of DMRT1 in the sex-determining region of the Y chromosome of the medaka, Oryzias latipes[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(18): 11778-11783. DOI: 10.1073/pnas.182314699.
[7]ZHANG X, GUAN G J, LI M Y, et al. Autosomal gsdf acts as a male sex initiator in the fish medaka[J]. Scientific Reports, 2016, 6:19738. DOI: 10.1038/srep19738.
[8]亢逸, 关桂君, 洪云汉. 用模式生物青鳉概观硬骨鱼性别决定及性分化研究进展[J]. 遗传, 2017, 39(6): 441-454. DOI: 10.16288/j.yczz.17-140.
[9]HINMAN M N, LOU H. Diverse molecular functions of Hu proteins[J]. Cellular and Molecular Life Sciences, 2008, 65(20): 3168-3181. DOI: 10.1007/s00018-008-8252-6.
[10]HINMAN M N, ZHOU H L, SHARMA A, et al. All three RNA recognition motifs and the hinge region of HuC play distinct roles in the regulation of alternative splicing[J]. Nucleic Acids Research, 2013, 41(9): 5049-5061. DOI: 10.1093/nar/gkt166.
[11]INCE-DUNN G, OKANO H J, JENSEN K B, et al. Neuronal elav-like (Hu) proteins regulate RNA splicing and abundance to control glutamate levels and neuronal excitability[J]. Neuron, 2012, 75(6): 1067-1080. DOI: 10.1016/j.neuron.2012.07.009.
[12]WU X, SCHMIDT J A, AVARBOCK M R, et al. Prepubertal human spermatogonia and mouse gonocytes share conserved gene expression of germline stem cell regulatory molecules[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(51): 21672-21677. DOI: 10.1073/pnas.0912432106.
[13]CHALUPNIKOVA K, SOLC P, SULIMENKO V, et al. An oocyte-specific ELAVL2 isoform is a translational repressor ablated from meiotically competent antral oocytes[J]. Cell Cycle, 2014, 13(7): 1187-1200. DOI: 10.4161/cc.28107.
[14]YANG C, YAO C C, JI Z Y, et al. RNA-binding protein ELAVL2 plays post-transcriptional roles in the regulation of spermatogonia proliferation and apoptosis[J]. Cell Proliferation, 2021, 54(9):e13098. DOI: 10.1111/cpr.13098.
[15]ZHANG X T, CHANG Y Y, ZHAI W Y, et al. A potential role for the Gsdf-eEf1α complex in inhibiting germ cell proliferation: a protein-interaction analysis in medaka (Oryzias latipes) from a proteomics perspective[J]. Molecular & Cellular Proteomics, 2021, 20:100023. DOI: 10.1074/mcp.RA120.002306.
[16]谢佳乐. 青鳉(Oryzias latipes)胰岛素样生长因子igf3的克隆和表达模式分析[D]. 上海: 上海海洋大学,2020.
[17]李茜, 王光幸, 李新闻,等. 青鳉gsdf调控ddx6介导的卵子发生的潜在机制[J]. 基因组学与应用生物学, 2022, 41(11): 2099-2110. DOI: 10.13417/j.gab.041.002099.
[18]QI C, WHEELER J A, PRUETT A, et al. Expression of the RNA binding proteins, Mel-N1, Mel-N2, and Mel-N3 in adipose cells[J]. Biochemical and Biophysical Research Communications, 2002, 294(2): 329-333. DOI: 10.1016/S0006-291X(02)00472-2.
[19]WU X W, ZHANG Y Q, XU S M, et al. Loss of Gsdf leads to a dysregulation of Igf2bp3-mediated oocyte development in medaka[J]. General and Comparative Endocrinology, 2019, 277: 122-129. DOI: 10.1016/j.ygcen.2019.04.001.
[20]YAN Y L, DESVIGNES T, BREMILLER R, et al. Gonadal soma controls ovarian follicle proliferation through Gsdf in zebrafish[J]. Developmental Dynamics, 2017, 246(11): 925-945. DOI: 10.1002/dvdy.24579.
[21]JIANG D N, YANG H H, LI M H, et al.Gsdf is a downstream gene of dmrt1 that functions in the male sex determination pathway of the Nile tilapia[J]. Molecular Reproduction and Development, 2016, 83(6): 497-508. DOI: 10.1002/mrd.22642.
[22]IMAI T, SAINO K, MATSUDA M. Mutation of gonadal soma-derived factor induces medaka XY gonads to undergo ovarian development[J]. Biochemical and Biophysical Research Communications, 2015, 467(1): 109-114. DOI: 10.1016/j.bbrc.2015.09.112.
[23]DI STEFANO B, LUO E C, HAGGERTY C, et al. The RNA helicase DDX6 controls cellular plasticity by modulating P-body homeostasis[J]. Cell Stem Cell, 2019, 25(5): 622-638.e13. DOI: 10.1016/j.stem.2019.08.018.
[24]KATO Y, IWAMORI T, NINOMIYA Y, et al. ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles[J]. EMBO Reports, 2019, 20(12). DOI: 10.15252/embr.201948251.
[25]KATO Y, SAGA Y. Antagonism between DDX6 and PI3K-AKT signaling is an oocyte-intrinsic mechanism controlling primordial follicle growth[J]. Biology of Reproduction, 2023, 109(1): 73-82. DOI: 10.1093/biolre/ioad043.
[26]GUAN G J, SUN K Q, ZHANG X, et al. Developmental tracing of oocyte development in gonadal soma-derived factor deficiency medaka (Oryzias latipes) using a transgenic approach[J]. Mechanisms of Development, 2017, 143: 53-61. DOI: 10.1016/j.mod.2016.12.006.
[27]LI H X, LIANG Z Q, YANG J, et al. DAZL is a master translational regulator of murine spermatogenesis[J]. National Science Review, 2019, 6(3): 455-468. DOI: 10.1093/nsr/nwy163.
[28]ROSARIO R, CRICHTON J H, STEWART H L, et al. Dazl determines primordial follicle formation through the translational regulation of Tex14[J]. FASEB Journal, 2019, 33(12): 14221-14233. DOI: 10.1096/fj.201901247R.
[29]GOOD P J. A conserved family of elav-like genes in vertebrates[J]. Proceedings of the National Academy of Sciences of the United States of America, 1995, 92(10): 4557-4561. DOI: 10.1073/pnas.92.10.4557.
[1] 王光幸, 郭振华, 杨继辉, 李雅园, 李新闻, 关桂君. 青鳉gsdf调控Igf2bp2/Igf2bp3-m6A阅读器在雌雄生殖细胞的差异表达[J]. 广西师范大学学报（自然科学版）, 2024, 42(2): 183-191.
[2] 李雅园, 王光幸, 李新闻, 郭振华, 关桂君. 青鳉nup58的表达及其在性别分化中的潜在作用[J]. 广西师范大学学报（自然科学版）, 2024, 42(1): 168-179.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 广西师范大学学报（自然科学版）编辑部
地址：广西桂林市三里店育才路15号　邮编：541004
电话：0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发