伴矿景天组培快繁体系研究

doi:10.16088/j.issn.1001-6600.2025041501

Abstract

Abstract: Using stem segments of Sedum plumbizincicola as explants and MS as the basic medium, the effects of different combinations of plant growth regulators on the disinfection, callus induction, adventitious bud induction, and rooting of S. plumbizincicola explants were studied. A rapid propagation system for S. plumbizincicola tissue culture was established. The results showed that the best disinfection effect was achieved by soaking in 75% alcohol for 30 seconds followed by immersion in 1 g/L HgCl₂ solution for 7 minutes. The combination of 0.5 mg/L NAA and 3.0 mg/L 6-BA was the most effective for callus induction. The combination of 3.0 mg/L NAA and 0.2 mg/L 6-BA yielded the best results for adventitious bud induction and proliferation, with a proliferation coefficient of 16.13. The best rooting effect for adventitious buds was 1.0 mg/L IBA, and the survival rate of transplanted seedlings exceeded 90%.

Key words: Sedum plumbizincicola, tissue culture, rapid propagation system, soil remediation, phytoremediation

CLC Number: Q943.1

WEI Xiulian, WEI Liyuan, HUANG Jia, HUANG Jing, LUO Mengwei, LI Bolin, CHEN Zhe. Research on Tissue Culture and Rapid Propagation Systemof Sedum plumbizincicola[J].Journal of Guangxi Normal University(Natural Science Edition), 2026, 44(2): 253-263.

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Research on Tissue Culture and Rapid Propagation Systemof Sedum plumbizincicola

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