Journal of Guangxi Normal University(Natural Science Edition) ›› 2018, Vol. 36 ›› Issue (2): 87-93.doi: 10.16088/j.issn.1001-6600.2018.02.012

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Monosaccharide Constituents of the Polysaccharides from the Leaf of Cyclocarya paliurus

JIANG Xianghui, JIANG Tianzhi, WANG Xiang, YUAN Jing*   

  1. College of Chemistry and Materials Engineering, Kaili University, Kaili Guizhou 556011, China
  • Online:2018-05-10 Published:2018-07-18

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Abstract: The polysaccharides were extracted by water extraction and alcohol precipitation method. Phenol sulfuric method was used for the determination of the content of polysacchrides. The monosacchride constituents of polysacchrides in the leaf of [WTBX]Cyclocarya paliurus[WTBZ] were determined by pre column derivatization HPLC. The content and composition of polysaccharides and monosaccharide from three different sources of Cyclocarya paliurus were analyzed and compared. The results displayed an average content of 4.34% for polysaccharides. The monosaccharide constituents of polysaccharides from the leaf of Cyclocarya paliurus are xylose, fructose, rhamnose, arabinose, galactose, glucose and ribose with their contents in polysaccharides being 3.04%，7.35%，8.46%，13.46%，24.32%，24.26% and 6.16%, respectively. The monosaccharide constituents and the content of polysaccharides show differences among different sources of Cyclocarya paliurus . It provides a basis for quality control and pharmacological efficacy study of Cyclocarya paliurus.

Key words: Cyclocarya paliurus, pre column derivatization method, HPLC, polysaccharides, monosaccharide constituents

CLC Number: 

  • Q532
[1] ZHU Kunning, JIANG Conghui, TIAN Yangsun, et al. Two triterpeniods from Cyclocarya paliurus (Batal) Iljinsk (Juglandaceae) promote glucose uptake in 3T3-L1 adipocytes: the relationship to AMPK activation[J]. Phytomedicine, 2015, 6: 1-10. DOI: 10.1016/j.phymed.2015.05.058.
[2] XIE Mingyong, LI Lei. Review in studies on chemical constituents and bioactivities of Cyclocarya paliurus[J]. Chin Tradit Herb Drugs, 2001, 32: 365-366. DOI:10.3321/j.issn:0253-2670.2001.04.039.
[3] DONG Congjian, XIE Minyong, NIE Songpin, et al. Study on antioxidant activity of Cyclocarya paliurus (Batal.) Iljinskaya extracts in vitro[J]. Food Sci, 2007, 28: 31-34. DOI:10.3321/j.issn:1002-6630.2007.10.001.
[4] JIANG Cao, YAO Ni, WANG Qin, et al. Cyclocarya paliurus extract modulates adipokine expression and improves insulin sensitivity by inhibition of inflammation in mice[J]. J Ethnopharmacol, 2014, 153: 344-351. DOI: 10.1016/j.jep.2014.02.003.
[5] XIE Mao, XIE Jin. Review about the research on Cyclocarya paliurus (Batal.) Il-jinskaja[J]. J Food Sci Biotechnol, 2008, 27: 113-121. DOI:10.3321/j.issn:1673-1689.2008.01.021.
[6] HUANG Mengqin, SHANGGUAN Xiaocao, XU Miaoshen, et al. Studies on hypolipemic effect of Cyclocarya paliurus (Batal) Iljinskaja polysaccharide[J]. Acta Agric Univ Jiangxiensis, 2011, 33: 157-161. DOI:10.3969/j.issn.1000-2286.2011.01.029.
[7] DENG Bing, SHANG Xunli, FANG Songzi, et al. Integrated effects of light intensity and fertilization on growth and flavonoid accumulation in Cyclocarya paliurus[J]. J Agric Food Chem, 2012, 60: 6286-6292. DOI: 10.1021/jf301525s.
[8] ZHA Xinqi, XUE Lao, ZHANG Huilao, et al. Molecular mechanism of a new Laminaria japonica polysaccharide on the suppression of macrophage foam cell formation via regulating cellular lipid metabolism and suppressing cellular inflammation[J]. Mol Nutr Food Res, 2015, 59: 2008-2021. DOI: 10.1002/mnfr.201500113.
[9] XIAO Jing, XING Fei, HUO Jing, et al. Lycium barbarum polysaccharides therapeutically improve hepatic functions in non-alcoholic steatohepatitis rats and cellular steatosis model[J]. Sci Rep, 2014, 4: 5587. DOI:10.1038.srep.05587.
[10] 向东,梁平,赖凤英. 南瓜多糖的理化性质及单糖组成[J].食品工业科技,2004, 12(12):62-63. DOI:10.3969/j.issn.1002-0306.2004.12.028.
[11] ZHANG Mei, CUI Songwun, CHEUNG Pingcun, et al. Antitumor polysaccharides from mushrooms: a review on their isolation process, structural character-istics and antitumor activity[J]. Trends Food Sci Technol, 2007,18(1): 4-19. DOI:10.1016/j.tifs.2006.07.013.
[12] WANG Xibiao, ZHAO Yong, WANG Qiwei, et al. Analysis of the monosaccharide components in Angelica polysaccharides by high performance liquid chromatography[J]. Anal Sci, 2005, 21(10): 1177-1180. DOI: 10.2116/analsci.21.1177.
[13] 陈木森,洪艳平,上官新晨. 青钱柳多糖的提取及含量测定[J]. 西北农业学报,2007, 16(3):192-195. DOI:10.3969/j.issn.1004-1389.2007.03.047.
[14] 上官新晨,陈木森,徐睿庸,等. 超声波法提取青钱柳多糖[J].江西农业大学学报,2006,28(6):809-812. DOI:10.3969/j.issn.1000-2286.2006.06.001.
[15] 季宇彬,陈学军,汲晨锋,等. 芦笋多糖提取、单糖组分分析及定量测定[J].中草药,2006,37(8):1159-1161. DOI:10.3321/j.issn:0253-2670.2006.08.016.
[16] 林雪,贾敬芬,黄琳娟,等. RP-HPLC 用于芦荟多糖的单糖组成研究[J]. 食品科学,2006,27(4):192-195. DOI:10.3321/j.issn:1002-6630.2006.04.046.
[17] 李冰,封士兰,刘小花,等. HPLC测定红芪药材中红芪多糖的含量[J]. 中成药,2008,30(5):716-718. DOI:10.3969/j.issn.1001-1528.2008.05.029.
[18] XIE Jianhua, SHEN Miyang, NIE Sunpi, et al. Analysis of monosaccharide composition of Cyclocarya paliurus polysaccharide with anion exchange chromatography[J]. Carbohyd Polym, 2013, 98: 976-981. DOI: 10.1016/j.carbpol.2013.07.011
[19] LI Wen, FAN Lipeng, DING Songdun. Isolation, purification and structure of a new water-soluble polysaccharide from Zizyphus jujuba cv. Jinsixiaozao[J]. Carbohyd Polym, 2011, 83: 477-482. DOI:10.1016/j.carbpol.2010.08.014.
[20] 诸燕,斯金平,郭宝林. 人工栽培铁皮石斛多糖含量变异规律[J]. 中国中药杂志,2010,35(4):427. DOI:10.4268/cjcmm20100406.
[21] 林守二,黄丽英,俞晓玲. 柱前衍生化HPLC法分析人工栽培金线莲中多糖的单糖组成[J].福建医科大学学报,2016,50(3):148-154.
[22] 苑鹤,白燕冰,斯金平,等. 柱前衍生HPLC分析铁皮石斛多糖中单糖组成的变异规律[J].中国中药杂志,2011,36(18):2465-2470. DOI:10.4268/cjcmm20111803.
[23] 葛霞,陈婷婷,蔡教英,等.青钱柳多糖抗氧化活性的研究[J].中国食品学报,2011,11(5): 59-64. DOI:10.16429/j.1009-7848.2011.05.004
[24] 武忠伟, 许桂芳, 曹蓬勃, 等. 虫草与富硒虫草多糖的体外抗氧化活性[J]. 食品科学, 2011, 32(9): 76-78.
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