生长抑素Ⅱ型受体SSTR2蛋白的理化性质及生物信息学分析

doi:10.16088/j.issn.1001-6600.2021121402

Abstract

Abstract: The physical and chemical properties, signal peptides, transmembrane structure, secretory protein types, subcellular localization, phosphorylation site modification, secondary structure and protein interaction of the human somatostatin type Ⅱ receptor (SSTR2) were analyzed by online bioinformatics software such as NCBI, ExPASy, SignalP, PSORTII, SOPMA, STRING, SWISS-MODEL, and the phylogenetic tree of SSTR2 protein was established by MegAlign software. The results showed that, the protein encoded by the gene contains 369 amino acids, the molecular formula was C₁₈₉₈H₂₉₈₄N₄₇₀O₅₁₃S₂₃, the relative molecular weight was 41 332.79, the theoretical value of isoelectric point was 9.15 and the instability coefficient was 37.16. SSTR2 was a stable alkaline hydrophilic protein with no signal peptide and 7 transmembrane regions, which acts on the plasma membrane structure. The secondary structure was mainly α-Helix belonging to 7tmGPCRs superfamily and containing one 7tmA_SSTR2 domain; proteins interacting with SSTR2 include CORT, SST, NPY, GHRL, GNAI1, GNAI2, GNAI3, SHANK1, HIVEP2. SSTR2 gene and its encoding protein were highly conserved in evolution, and may be involved in the G protein coupled receptor signal pathway and play its role. In this study, the physical and chemical properties and biological functions of SSTR2 gene were analyzed to provide theoretical basis for the in-depth study of the mechanism of SSTR2 protein in the occurrence of diseases and the research and development of targets therapies for neuroendocrine tumors and other cancer drugs.

Key words: somatostatin receptor, SSTR2 protein, NETs, bioinformatics, biological function

CLC Number:

Q811.4

ZHANG Limeng, LI Runting, NIE Xiaoning, LI Yuhua, LI Lin, LI Yameng, CHEN Longxin, WANG Linqing. Physicochemical Properties and Bioinformatics Analysis of Somatostatin Receptor 2 (SSTR2)[J].Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(1): 164-173.

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Physicochemical Properties and Bioinformatics Analysis of Somatostatin Receptor 2 (SSTR2)

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