Journal of Guangxi Normal University(Natural Science Edition) ›› 2020, Vol. 38 ›› Issue (4): 124-131.doi: 10.16088/j.issn.1001-6600.2020.04.015

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Cloning and Embryonic Developmental Expression Analysis ofFollistatin Gene in Siniperca chuatsi

HU Jiangli1, XU Lei1, XU Yilan1, LIU Jingjie1, SUN Yue1, XIE Yandong1, BIN Shiyu1,2*   

  1. 1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Key Laboratory of Ecologyof Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministsry of Education,Guilin Guangxi 541006, China
  • Received:2018-12-27 Published:2020-07-13

Abstract: In order to investigate the expression of Follistatin (FST) gene in Siniperca chuatsi during embryonic development, the full-length cDNA sequence of FST gene was cloned by qRT-PCR, 3′RACE and 5′RACE techniques from the embryonic tissue of S. chuatsi (GenBank:KM077175.1). Bioinformatics analysis showed that the full length of cDNA is 1 271 bp with coding region 960 bp and encoding 319 amino acids. The amino acid sequence identity of FST and their phylogenetical relations with other species were also assayed. The expression profile of FST was analyzed with qRT-PCR at different embryonic development stages in S. chuatsi. The results showed that FST expression is relatively low at both fertilization and cleavage stages. After that, its expression increases significantly from blastocyst stage, reaches the highest at neural stage, and then its expression remains at relative low level.

Key words: Siniperca chuatsi, Follistatin, cloning, embryonic development, expression analysis

CLC Number: 

  • Q959.483
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