基于UPLC-QTOF-MS、网络药理学和实验验证探讨复方鹿仙草颗粒抗肝癌作用机制

doi:10.16088/j.issn.1001-6600.2024080505

Abstract

Abstract: The study aimed to explore the antitumor effects of Fufang Luxiancao Keli(FLK) on liver cancer and its underlying mechanisms. A total of 57 chemical components were identified using UPLC-QTOF-MS technology. Employing network pharmacology tools and molecular docking techniques, coupled with GO and KEGG analyses, it was revealed that the drug contains 37 active ingredients with 338 intersecting targets related to liver cancer. The core targets include PIK3CA, PIK3CB, PIK3CD, PIK3R1, and EGFR, which were involved in biological processes such as protein phosphorylation, cell migration, activation, and proliferation. KEGG analysis suggested that FLK may inhibit the growth of liver cancer cells by affecting key pathways such as the cancer pathway and the PI3K-Akt signaling pathway. In vitro cellular experiments confirmed the results of network pharmacology, demonstrating that FLK effectively inhibits the proliferation, cloning, and migration of liver cancer cells, and induces apoptosis and cell cycle arrest. The findings indicated that FLK may exert its antitumor effects by regulating signaling pathways such as PI3K-Akt.

Key words: Fufang Luxiancao Keli, liver cancer, UPLC-QTOF-MS, network pharmacology, molecular docking

CLC Number: R284

QIN Yehao, GUO Chenjing, WU Lichuan, WEI Pengcheng. Exploring the Anti-Liver Cancer Mechanism of Fufang Luxiancao Keli Based on UPLC-QTOF-MS, Network Pharmacology, and Experimental Validation[J].Journal of Guangxi Normal University(Natural Science Edition), 2025, 43(2): 221-237.

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Exploring the Anti-Liver Cancer Mechanism of Fufang Luxiancao Keli Based on UPLC-QTOF-MS, Network Pharmacology, and Experimental Validation

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