Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (3): 242-254.doi: 10.16088/j.issn.1001-6600.2022040403

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Effects of Cr(Ⅵ) Concentration on the Performance and Microecology of MFC-Granular Sludge Coupling System

QIN Ronghua1,2, SU Chengyuan1,2*, LU Xinya2, CHEN Zhengpeng2, ZHOU Yijie2, XIAN Yunchuan2   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. School of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2022-04-04 Revised:2022-05-05 Online:2023-05-25 Published:2023-06-01

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Abstract: Heavy metal pollution in water environment cannot be ignored, especially the pollution phenomenon of hexavalent chromium (Cr(Ⅵ)). In order to explore a new idea for efficient treatment of Cr(Ⅵ)-containing wastewater, a microbial fuel cell (MFC)-granular sludge coupling system was established. The effects of the Cr(Ⅵ) concentration on the performance and microecology of MFC-granular sludge coupling system were investigated. When the Cr(Ⅵ) content reached 80 mg/L in the influent, the chemical oxygen demand (COD) degradation and biological reduction efficiency of Cr(Ⅵ) by the system were 92.71% and 99.70%, respectively. In the electrochemical performance analysis, cyclic voltammetry (CV) showed an obvious reduction peak. Meanwhile, the electrochemical impedance spectroscopy (EIS) illustrated that with increasing Cr(Ⅵ) concentrations, the charge transfer resistance gradually decreased. When the influent Cr(Ⅵ) concentration increased to 100 mg/L, the lowest activity of coenzyme F420 was 0.026 2±0.000 2 mmol/g; in addition, the activity of INT-ETS sharply decreased to 5.20±0.23 μg/(mg·h). The morphology of Cr(Ⅵ) after biological reduction of chromate was determined by X-ray photoelectron spectroscopy (XPS), the binding energy of the characteristic peaks demonstrated that was mainly represented by Cr2O3 and Cr(OH)3. High-throughput sequencing analysis illustrated that the dominant microbial flora were Chloroflexi and Proteobacteria. When the concentration of Cr(Ⅵ) increased to 100 mg/L, the dominant microbial community structure were transformed from Methanothrix and Methanobacterium to Geobacter and Syntrophobacter at genus level, the maximum relative abundance of each bacteria were 21.24%, 4.28%, 5.60% and 6.22%, respectively. It was indicated that increasing the concentration of Cr(Ⅵ) would change the microbial community structure of the MFC-granular sludge coupling system, and Cr(Ⅵ) was reduced through the association mechanism between microbial communities.

Key words: chromate reduction, MFC-granular sludge coupling system, electrochemical performance, microbial communities

CLC Number:  X703.1
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