鳜RORα基因的胚胎发育特征及饥饿对其节律性表达影响分析

doi:10.16088/j.issn.1001-6600.2020110204

Abstract

Abstract: In order to explore the expression characteristics of RORα gene in Siniperca chuatsi and the effect of starvation on its rhythm, the expression of RORα in S. chuatsi different embryonic developmental stages and the circadian expression of RORα gene in muscle and liver of S. chuatsi after short-term starvation were detected by real-time quantitative PCR. The results showed that the expression of RORα gene was low in cleavage stage, higher in early blastula to early-gastrula stage, and lower in late-gastrula embryo to larval stage. There was no significant difference from late-gastrula embryo to larval stage except tail bud stage. The expression of RORα gene in muscle and liver of S. chuatsi showed obvious circadian rhythm, which was higher in the daytime and lower in the night; however, the circadian expression of RORα gene in muscle disappeared after 5 days of starvation. Although RORα gene showed circadian rhythm in liver, its median value, oscillation amplitude and peak phase had significant effects (P<0.05). In summary, RORα gene may play an important role in the blastula to early-gastrula stage of S. chuatsi. Furthermore, starvation stress could affect the circadian rhythm of RORα gene in muscle and liver of S. chuatsi, which may lead to the disorder of physiological process.

Key words: Siniperca chuatsi, RORα, embryonic development, starvation, circadian rhythm

CLC Number:

S917.4

LIU Jingjie, CHU Wuying, ZHU Xin, SUN Yue, XU Yilan, XIE Yandong, BIN Shiyu, ZHANG Jianshe. Embryonic Development Characteristics and Rhythmic Expression Analysis of RORα Gene under Starvation in Siniperca chuatsi[J].Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(5): 190-197.

References

[1] 鄢慧玲, 褚武英, 吴萍, 等. 鳜肝脏和心脏生物钟基因表达节律分析[J]. 水生生物学报, 2016, 40(2): 243-251. DOI:10.7541/2016.34.
[2] BARNEA M, MADAR Z, FROY O. High-fat diet delays and fasting advances the circadian expression of adiponectin signaling components in mouse liver[J]. Endocrinology, 2009, 150(1): 161-168. DOI:10.1210/en.2008-0944.
[3] 张方亮, 邓小红, 朱晓彤, 等. 翘嘴鳜USP7基因cDNA的克隆及饥饿对其表达影响分析[J]. 广西师范大学学报(自然科学版), 2018, 36(2): 141-147. DOI:10.16088/j.issn.1001-6600.2018.02.020.
[4] FARAHANI S, SOLGI L, BAYAT S, et al. RAR-related orphan receptor A: One gene with multiple functions related to migraine[J]. CNS Neuroscience & Therapeutics, 2020, 26(12): 1315-1321. DOI:10.1111/cns.13453.
[5] SCHMUTZ I, RIPPERGER J A, BAERISWYL-AEBISCHER S, et al. The mammalian clock component PERIOD₂coordinates circadian output by interaction with nuclear receptors[J]. Genes & Development, 2010, 24(4): 345-357. DOI:10.1101/gad.564110.
[6] LI J C, ZOU X M. MiR-652 serves as a prognostic biomarker in gastric cancer and promotes tumor proliferation, migration, and invasion via targeting RORα[J]. Cancer Biomarkers, 2019, 26(3): 323-331. DOI:10.3233/CBM-190361.
[7] LI Z Q, ZHAO J, LIU H Y, et al. Melatonin inhibits apoptosis in mouse Leydig cells via the retinoic acid-related orphan nuclear receptor α/p53 pathway[J]. Life Sciences, 2020, 246: 117431. DOI:10.1016/j.lfs.2020.117431.
[8] FLORES M V, HALL C, JURY A, et al. The zebrafish retinoid-related orphan receptor (ror) gene family[J]. Gene Expression Patterns, 2007, 7(5): 535-543. DOI:10.1016/j.modgep.2007.02.001.
[9] SOLT L A, KOJETIN D J, BURRIS T P. The REV-ERBs and RORs: molecular links between circadian rhythms and lipid homeostasis[J]. Future Medicinal Chemistry, 2011, 3(5): 623-638. DOI:10.4155/fmc.11.9.
[10] LAU P, FITZSIMMONS R L, RAICHUR S, et al. The orphan nuclear receptor, RORα, regulates gene expression that controls lipid metabolism: staggerer (SG/SG) mice are resistant to diet-induced obesity[J]. The Journal of Biological Chemistry, 2008, 283(26): 18411-18421. DOI:10.1074/jbc.M710526200.
[11] ITOH Y, FUCHINO H, SANOSAKA M, et al. Pterosin B has multiple targets in gluconeogenic programs, including coenzyme Q in RORα-SRC2 signaling[J]. Biochemical and Biophysical Research Communications, 2016, 473(2): 415-420. DOI:10.1016/j.bbrc.2016.03.016.
[12] YANG Y, DONG F S, LIU X G, et al. Dysregulation of circadian rhythm in zebrafish (Danio rerio) by thifluzamide: Involvement of positive and negative regulators[J]. Chemosphere, 2019, 235: 280-287. DOI:10.1016/j.chemosphere.2019.06.153.
[13] TAIRA G, ONOUE T, HIKIMA J-I, et al. Circadian clock components Bmal1 and Clock1 regulate tlr9 gene expression in the Japanese medaka (Oryzias latipes)[J]. Fish & Shellfish Immunology, 2020, 105: 438-445. DOI:10.1016/j.fsi.2020.07.009.
[14] LAZADO C C, VOLDVIK V. Temporal control of responses to chemically induced oxidative stress in the gill mucosa of Atlantic salmon (Salmo salar)[J]. Journal of Photochemistry and Photobiology B: Biology, 2020, 205: 111851. DOI:10.1016/j.jphotobiol.2020.111851.
[15] MARTINS R S T, GOMEZ A, ZANUY S, et al. Photoperiodic modulation of circadian clock and reproductive axis gene expression in the pre-pubertal European sea bass brain[J]. PLoS ONE, 2015, 10(12): e144158. DOI:10.1371/journal.pone.0144158.
[16] 王开卓, 宾石玉, 李虹辉, 等. 翘嘴鳜生长抑制素基因的克隆及其表达分析[J]. 广西师范大学学报(自然科学版), 2013, 31(4): 109-114. DOI:10.16088 /j.issn.1001-6600.2013.04.017.
[17] 刘希良, 宾石玉, 王开卓, 等. 翘嘴鳜的人工繁殖与胚胎发育观察[J]. 广西师范大学学报(自然科学版), 2013, 31(2): 100-106. DOI:10.16088 /j.issn.1001-6600.2013.02.009.
[18] 徐蕾, 陈小保, 唐春, 等. 翘嘴鳜UCP3基因cDNA的克隆及其胚胎发育表达分析[J]. 广西师范大学学报(自然科学版), 2017, 35(1): 92-97. DOI:10.16088/j.ssn.1001-6600.2017.01.015.
[19] BROWN R A M, EPIS M R, HORSHAM J L, et al. Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal[J]. BMC Biotechnology, 2018, 18: 16. DOI:10.1186/s12896-018-0421-6.
[20] ZHOU R X, MENG T, MENG H B, et al. Selection of reference genes in transcription analysis of gene expression of the mandarin fish, Siniperca chuasti[J]. Zoological Research, 2010, 31(2): 141-146. DOI:10.3724/SP.J.1141.2010.02141.
[21] WU P, LI Y L, CHENG J, et al. Daily rhythmicity of clock gene transcript levels in fast and slow muscle fibers from Chinese perch (Siniperca chuatsi)[J/OL]. BMC Genomics, 2016, 17(1):1008.
[22] 胡姜丽, 徐蕾, 许艺兰, 等. 鳜Follistatin基因cDNA的克隆及其胚胎发育表达分析[J]. 广西师范大学学报(自然科学版), 2020, 38(4): 124-131. DOI:10.16088/j.issn.1001-6600.2020.04.015.
[23] 农小献, 赵发兰, 宾石玉, 等. 斑鳜轻链3基因(MLC3)cDNA的克隆及纵向表达分析[J]. 湖南师范大学自然科学学报, 2011, 34(2): 79-83.
[24] 陈圆华, 李虹辉, 王利香, 等. GSK4112对尼罗罗非鱼肝脏Nr1d1和Ulk1b基因昼夜节律性表达的影响[J/OL]. 基因组学与应用生物学, 2019: 1-9[2021-03-19]. http:// kns.cnki.net/kcms/detail/45.1369.q.20191120.1333.006.html.
[25] GOLD D A, GENT P M, HAMILTON B A. RORα in genetic control of cerebellum development: 50 staggering years[J]. Brain Research, 2007, 1140(6): 19-25. DOI:10.1016/j.brainres.2005.11.080.
[26] PARAVICINI G, STEINMAYR M, ANDRÉ E, et al. The metastasis suppressor candidate nucleotide diphosphate kinase NM23 specifically interacts with members of the ROR/RZR nuclear orphan receptor subfamily[J]. Biochemical and Biophysical Research Communications, 1996, 227(1): 82-87. DOI:10.1006/bbrc.1996.1471.
[27] 王晓龙, 温海深, 张美昭, 等. 花鲈初孵仔鱼饥饿不可逆点的确定及摄食节律研究[J]. 中国海洋大学学报(自然科学版), 2017, 47(5): 57-64. DOI:10.16441/j.cnki.hdxb.20160100.
[28] 宾石玉, 林勇, 曾兰, 等. 低温胁迫对埃及尼罗罗非鱼肝、脾和鳃细胞形态的影响[J]. 广西师范大学学报(自然科学版), 2015, 33(3): 123-128. DOI:10.16088/j.issn.1001-6600.2015.03.019.
[29] 汪建华. 短期饥饿对锦鲫肌肉和肝脏中生物钟相关基因表达的影响[D]. 桂林: 广西师范大学, 2016.
[30] 汪建华, 李玉珑, 陈敦学, 等. 饥饿再投喂对鳜肌FSRP-1、FSRP-3和肠道PepT1基因表达的影响[J]. 广西师范大学学报(自然科学版), 2016, 34(1): 144-149. DOI:10.16088/j.issn.1001-6600.2016.01.023.
[31] LUCAS-SÁNCHEZ A, ALMAIDA-PAGÁN P F, MADRID J A, et al. Age-related changes in fatty acid profile and locomotor activity rhythms in Nothobranchius korthausae[J]. Experimental Gerontology, 2011, 46(12): 970-978. DOI:10.1016/j.exger.2011.08.009.
[32] REZNICK J, PRESTON E, WILKS D L, et al. Altered feeding differentially regulates circadian rhythms and energy metabolism in liver and muscle of rats[J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 2013, 1832(1): 228-238. DOI:10.1016/j.bbadis.2012.08.010.
[33] 宾石玉, 廖芳, 杜雪松, 等. 罗非鱼耐寒性能研究进展[J]. 广西师范大学学报(自然科学版), 2021, 39(1): 10-16. DOI:10.16088/j.issn.1001-6600.2020081801.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	XU Lunhui,YIN Shide,LIU Yijia. Self-Adaptive Cuckoo Algorithm Based on Simulated Annealing for Bus Scheduling Problem[J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(2): 1 -7 .
[2]	HUANG Li, ZOU Yanli, WANG Yi, LI Ke. A Comparative Study on Three Types of Distributed PowerPlant Connection Strategies[J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(3): 30 -36 .
[3]	CHEN Yun-kun, LI Xian-hua. 2-maximal Subgroups of Sylow Subgroups and p-nilpotency of Finite Groups[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(2): 31 -34 .
[4]	ZHANG Guo-feng, YU Tao. Slant Toeplitz Operators on Dirichlet Space[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(2): 50 -55 .
[5]	LUO Xin, PAN Qiao, WANG Hong-ya, CHEN Mei, KITA Kenji. Realization of High-speed Image Search Based on SOFM[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(2): 180 -184 .
[6]	XIONG Shun-qing, ZHOU Wei-hong. New Digital Watermarking Algorithm Based on NSCT and SVD[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(2): 195 -199 .
[7]	ZHONG Xiang-gui, LI Yong-gang, ZHANG Fu-sheng, WUYong. Finite Nilpotent Groups with Automorphism Group of Order 16p₁p₂…p_r[J]. Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(2): 58 -63 .
[8]	HUA Shi-xian, WANG Ying-chun, LI Ya-jun, WANG Heng-shan. Synthesis and Optical Properties of 12-Aryl Substituted Derivatives of Methyl Dehydroabietate[J]. Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(2): 64 -68 .
[9]	LIU Xi-liang, BIN Shi-yu, WANG Kai-zhuo, CHEN Dun-xue, CHENG Jia, ZHANG Jian-she, CHU Wu-ying. Artificial Propagation and Embryonic Development Observation of Mandarin Fish[J]. Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(2): 100 -106 .
[10]	YANG Li, KONG Ling-jiang. Capillary Force between Microparticles[J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(1): 1 -4 .

Embryonic Development Characteristics and Rhythmic Expression Analysis of RORα Gene under Starvation in Siniperca chuatsi

PDF (PC)

Abstract

Cite this article

share this article

References

Related Articles 8

Metrics

Comments

Recommended 10

[1]	LUO Honglin, FENG Pengfei, YU Yanling, XIAO Rui, PAN Chuanyan, SONG Manling, ZHANG Yongde. Molecular Cloning of the Myostatin Gene and Its Expression During Embryo Development of Trachinotus ovatus [J]. Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(1): 136-147.
[2]	HU Jiangli, XU Lei, XU Yilan, LIU Jingjie, SUN Yue, XIE Yandong, BIN Shiyu. Cloning and Embryonic Developmental Expression Analysis ofFollistatin Gene in Siniperca chuatsi [J]. Journal of Guangxi Normal University(Natural Science Edition), 2020, 38(4): 124-131.
[3]	ZHANG Fangliang, DENG Xiaohong, ZHU Xiaotong, XU Lei, HU Jiangli, BIN Shiyu. Cloning and Expression Analysis of Ubiquitin-Specific Protease 7 Gene under Starvation in Mandarin Fish (Siniperca chuatsi) [J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(2): 141-147.
[4]	XU Lei,CHEN Xiaobao,TANG Chun,HU Jiangli,BIN Shiyu,ZHANG Fangliang. Cloning and Embryonic Development Expression Analysis of the Uncoupling Proteins 3 Gene in Mandarin Fish [J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(1): 92-97.
[5]	WANG Jianhua, LI Yulong, CHEN Dunxue, ZHU Xin, LIU Zhixing, ZHANG Jianshe, CHU Wuying, BIN Shiyu. Effect of Starvating and Refeeding on Relative Expression of FSRP-1,FSRP-3 and PepT1 Gene in Mandarin Fish (Siniperca chuatsi) [J]. Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(1): 144-149.
[6]	YI Tan , LIU Xi-liang, BIN Shi-yu, WANG Kai-zhuo, WU Ping, CHU Wu-ying, CHEN Tao. Molecular Cloning and Expression Analysis of Slow Myosin Heavy Chain 1 (sMyHC1) Gene cDNA in Mandarin Fish(Siniperca chuatsi) [J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(2): 175-180.
[7]	LIU Xi-liang, BIN Shi-yu, WANG Kai-zhuo, CHEN Dun-xue, CHENG Jia, ZHANG Jian-she, CHU Wu-ying. Artificial Propagation and Embryonic Development Observation of Mandarin Fish [J]. Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(2): 100-106.
[8]	CHEN Dun-xue, LI Yu-long, BIN Shi-yu, NONG Xiao-xian, LIU Xi-liang, CHENG Jia, ZHANG Jian-she, CHU Wu-ying. Characterization of the Complete Mitochondrial Genome Sequence in Siniperca chuatsi [J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(4): 111-116.