Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (4): 120-125.

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Blood Physiological and Biochemical Changes to Cold Stress in Tilapia

BIN Shi-yu1,2, ZENG Lan1, DU Xue-song1,2, ZHANG Yan1,2, LIN Yong1, TANG Zhang-sheng1, ZHANG Yong-de1, YANG Hui-zan1, CHEN Zhong1   

  1. 1.Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Guangxi Academy of Fishery Sciences, Nanning Guangxi 530021, China;
    2.College of Life Science, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2014-08-02 Published:2018-09-26

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Abstract: To investigate the effects of cold stress on blood physiological and biochemical changes of tilapia, Egyptian strain Nile tilapia, American strain Nile tilapia and Oreochromis aureus were selected to study the effects of different cold stress on blood physiological and biochemical changes using artificial indoor cooling. There are 90 tilapia in each type. The average body weight of tilapia was (150±2) g. These samples were randomly divided into 25 ℃, 15 ℃ and 12 ℃ 3 groups with three repeats, 10 tilapias for each repeat. The results showed that the number of red blood cells (RBC), hemoglobin (HGB), mean corpuscular hemoglobin (MCH) and hematocrit (HCT) in three kinds of tilapia showed no significant difference (P>0.05) under 15 ℃ for 0 d, 5 d, 10 d, and coma, and under 12 ℃ for 0 h, 36 h, 72 h compared with the control group. The average hematocrit (MCV) was significantly decreased (P<0.05), and mean corpuscular hemoglobin concentration (MCHC) and serum total protein (TP) concentrations were significantly increased (P<0.05). Albumin (ALB) concentration decreased with the decreased temperature but with no significant difference with control group (P>0.05). Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations increased with the decreasing temperature. Superoxide dismutase (SOD) activity increased (P>0.05) at 15 ℃ for 0 d, 5 d, 10 d and coma, but decreased at 12 ℃ for 0 h, 36 h, and 72 h.

Key words: cold stress, tilapia, blood, physiological and biochemical parameters

CLC Number: 

  • Q954.63
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