Journal of Guangxi Normal University(Natural Science Edition) ›› 2013, Vol. 31 ›› Issue (1): 119-124.

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Screening and Identification of a Strain of Hydantoinase-producing Microorganism

WANG Pei1,2, CAO Jian-hua1, SONG De-gui2, GU Lan-tao2, DONG Yan-ling3   

  1. 1.Karst Dynamics Laboratory,Ministry of Land and Resource,Institute of Karst Geology,Chinese Academy of GeologicalSciences,Guilin Guangxi 541004,China;
    2.College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China;
    3.Department of Biotechnology,Wuhan Bioengineering Institute,Wuhan Hubei 430415,China
  • Received:2012-11-19 Online:2013-03-20 Published:2018-11-26

Abstract: Through gathering,isolation and purification,a strain of Hydantoinase-producing microorganisms from soil microbial based on single hydantoin analogue was taken as the onlynitrogen source.The Hydantoinase-producing microorganisms was screened by utilizing the Double agar-agar method.Then,it DNAwas extracted by the means ofgenetic engineering method,and sequence of its specificity 16S rDNA was tested.Lastly,a comparison its gene sequence to GenBank database byBlastn tool.Research result shows:this microorganism has 98% similarity with Bacillus megaterium QM B1551 (NC004604),which proved that it is Bacillus megaterium.

Key words: hydantoinase, double agar-agar method, 16S rDNA, Bacillus megaterium

CLC Number: 

  • Q93-331
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