Journal of Guangxi Normal University(Natural Science Edition) ›› 2010, Vol. 28 ›› Issue (3): 37-41.

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Development of a Novel HPLC-RRS Method for Determination of Gentamicin Components

LIU Cheng-wei1, LUO Zhi-hui2,3, LU Wei-tian1, YU Lan1, LU Xin2, ZHAO Shu-lin2   

  1. 1. College of Basic Medicine,Guilin Medical University,GuilinGuangxi 541004,China;
    2. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Ministry of Education, Guangxi Normal University,Guilin Guangxi 541004,China;
    3. College of Chemistry and Chemical Engineering, Guangxi Normal University,Guilin Guangxi 541004,China;
    4. Department of Chemistry and Biology, Yulin Normal University,Yulin Guangxi 537000,China
  • Received:2010-05-27 Online:2010-09-20 Published:2023-02-06

Abstract: A selective and rapid high-performance liquid chromatography coupled with resonance Rayleigh scattering detection method was developed for the determination of multicomponent gentamicin (Gen).Pontamine sky blue was chosen as molecular recognition probe,which could forms ion-association complex with Gen inmobile medium and produce strong scattering signal.The separation of Gen components was achieved on a C18 column and the column eluent was monitored usingfluorescence detection at λex=λem=356 nm.The detection limitsof 6.0～7.2 mg/L were reached,and the response linearity between peak area and components concentration were found in the range of 7.2～115,8.2～132,7.5～79 and 8.5～78 mg/L for C1,C1a,C2,C2a,respectively.This method has been applied to the determination of Gen components in commercial Gen sulfate injection,and the detection results were in good agreement with the specification of Chinese Pharmacopoeia.

Key words: high-performance liquid chromatography, resonance Rayleigh scattering, gentamicin, pontamine sky blue

CLC Number: 

  • O652.63
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