对菌根分泌物强趋性产酸克氏菌突变体的筛选

广西师范大学学报（自然科学版） ›› 2014, Vol. 32 ›› Issue (2): 130-136.

对菌根分泌物强趋性产酸克氏菌突变体的筛选

于晓宇¹, 江绍锋¹, 蓝运华¹, 夏樱花¹, 李云飞¹, 陆祖军^1,2,3

1.广西师范大学生命科学学院,广西桂林541004;
2.珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004;
3.广西高校野生动植物生态学重点实验室,广西桂林541004

收稿日期:2013-12-09 出版日期:2014-06-25 发布日期:2018-09-25
通讯作者: 陆祖军(1963—),男(壮族),广西平果人,广西师范大学教授,博士。E-mail:luzujun2002@yahoo.com.cn
基金资助:
国家自然科学基金资助项目(31060120);广西自然科学基金资助项目(2013GXNSFAA019059);广西教育厅科学技术研究项目(2009-25);珍稀濒危物种和生态环境保护重点实验室(广西师范大学)研究基金资助项目;中国教育部和国家科技支撑计划资助项目(2012BAC16B03)

Screening for Mutants of Klebsilla oxytoca with Stronger Chemotaxis to Exudates from Mycorrhiza of Eucalyptus-Pisolithus tinnctorius

YU Xiao-yu¹, JIANG Shao-feng¹, LAN Yun-hua¹, XIA Ying-hua¹, LI Yun-fei¹, LU Zu-jun^1,2,3

1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China;
2.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,China,Guilin Guangxi 541004,China;
3.Key Ecological Laboratory of Wild Animal and Plant Species of Guangxi Collegesand Universities,Guilin Guangxi 541004,China

Received:2013-12-09 Online:2014-06-25 Published:2018-09-25

摘要/Abstract

摘要： 体积分数0.01%和0.001%的桉树油悬浮液与苗期桉树-彩色豆马勃菌根、桉树根、彩色豆马勃菌Pisolithus tinnctorius分泌物对随机突变的产酸克氏菌Klebsilla oxytoca进行趋性筛选,得到1株较野生株对桉树-彩色豆马勃菌菌根分泌物趋向性强1~2倍的突变株菌株TR-M30-1。该菌株为建立桉树-彩色豆马勃菌-产酸克氏菌三元互利共生体系提供了材料。

关键词: 桉树, 产酸克氏菌, 菌根, 分泌物, 趋性

Abstract: Mutant TR-M30-1 of Klebsilla oxytoca showing 1-2 times stronger chemotaxis than its wild strain to the exudates of mycorrhiza of Eucalyptus-Pisolithus tinnctorius was screened by first using 0.1 mL/L and 0.01 μL/mL of Eucalyptus oil suspensions as the chemotaxin then the secretions of Pisolithus tinctorius, root of Eucalyptus seedings and mycorrhiza of Eucalyptus-Pisolithus tinnctorius. These results provided the initial possibility for establishing three-way mutualism system of Eucalyptus-Pisolithus tinctorius-K. oxytoca.

Key words: Eucalyptus, Klebsilla oxytoca, mycorrhiza, secretion, chemotaxis

中图分类号:

Q939.96

于晓宇, 江绍锋, 蓝运华, 夏樱花, 李云飞, 陆祖军. 对菌根分泌物强趋性产酸克氏菌突变体的筛选[J]. 广西师范大学学报（自然科学版）, 2014, 32(2): 130-136.

YU Xiao-yu, JIANG Shao-feng, LAN Yun-hua, XIA Ying-hua, LI Yun-fei, LU Zu-jun. Screening for Mutants of Klebsilla oxytoca with Stronger Chemotaxis to Exudates from Mycorrhiza of Eucalyptus-Pisolithus tinnctorius[J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(2): 130-136.

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