水稻细菌性条斑病菌一个新基因与致病相关

广西师范大学学报（自然科学版） ›› 2014, Vol. 32 ›› Issue (2): 137-142.

水稻细菌性条斑病菌一个新基因与致病相关

黄胜^1,2, 卢烨¹, 朱晓琳¹, 潘俊霞¹, 何勇强^1,2, 姜伟^1,2

1. 广西大学生命科学与技术学院, 广西南宁530004;
2.亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004

收稿日期:2014-01-06 出版日期:2014-06-25 发布日期:2018-09-25
通讯作者: 姜伟(1975—),男(瑶族),广西桂林人,广西大学副教授,博士。E-mail:weijiang@gxu.edu.cn
基金资助:
国家自然科学基金资助项目(31260261、 31360021);广西自然科学基金资助项目(20130231);广西教育厅基金资助项目(20130300)

A New Gene is Required for Full Virulence of Xanthomonas oryzae pv. oryzicola in Rice

HUANG Sheng^1,2, LU Ye¹, ZHU Xiao-lin¹, PAN Jun-xia¹, HE Yong-qiang^1,2, JIANG Wei^1,2

1.College of Life Science and Technology, Guangxi University,Nanning Guangxi 530004,China;
2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-biosciences, Nanning Guangxi 530004, China

Received:2014-01-06 Online:2014-06-25 Published:2018-09-25

摘要/Abstract

摘要： 水稻细菌性条斑病菌是水稻的主要病原菌之一,其引发的水稻细菌性条斑病可造成水稻严重减产。利用广西分离株GX01作为建库出发菌株,采用EZ∷Tn5转座子标签法构建GX01菌株的Tn5随机插入突变体库,其后利用针刺接种法在水稻上进行致病性试验,筛选到1个致病性明显下降的突变体。TAIL-PCR定位该突变位点位于XOC3376,该基因的编码产物注释为假设蛋白。为了研究XOC3376的功能,对其进行表型检测及功能互补,结果表明其互补菌株的表型及致病力都能恢复到野生型的水平。本文为进一步研究水稻细菌性条斑病菌的致病相关基因的功能、阐明该病菌的致病机理奠定基础。

关键词: 水稻细菌性条斑病菌, EZ∷Tn5转座子, 突变体库, TAIL-PCR, 致病相关基因

Abstract: Xanthomonas oryzae pv. oryzicola (hereafter Xoc) is one of the main pathogens of rice, which could cause rice bacterial leaf streak, resulting in severe losses in rice production. In this study, a random insertion mutant library of Xoc strain which isolated from Guangxi and named as GX01 was constructed using EZ∷Tn5 transposon. One mutant which showed greatly reduced virulence on rice was identified from the library using puncture virulence assay. The insertion site was mapped by TAIL-PCR in XOC3376, and this gene encodes a hypothetical protein. In order to investigate this genes’s functions, several phenotype and virulence assay of this mutant and complementary strain were checked. The results showed that all phenotype of the complementary strain and virulence have been recovered. The study facilitate elucidating the pathogenesis of this phytopathogen.

Key words: Xanthomonas oryzae pv. oryzicola, EZ∷Tn5 transposon, mutant library, TAIL-PCR, pathogenicity genes

中图分类号:

黄胜, 卢烨, 朱晓琳, 潘俊霞, 何勇强, 姜伟. 水稻细菌性条斑病菌一个新基因与致病相关[J]. 广西师范大学学报（自然科学版）, 2014, 32(2): 137-142.

HUANG Sheng, LU Ye, ZHU Xiao-lin, PAN Jun-xia, HE Yong-qiang, JIANG Wei. A New Gene is Required for Full Virulence of Xanthomonas oryzae pv. oryzicola in Rice[J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(2): 137-142.

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