加拿大一枝黄花和南美蟛蜞菊精油的杀虫活性及化学成分

广西师范大学学报（自然科学版） ›› 2014, Vol. 32 ›› Issue (2): 122-129.

加拿大一枝黄花和南美蟛蜞菊精油的杀虫活性及化学成分

邓业成^1,2, 李瑞钰^1,2, 杨林林^1,2, 钟慧^1,2, 周秋艳^1,2, 卿朕^1,2

1.珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004;
2.广西师范大学生命科学学院, 广西桂林541004

收稿日期:2013-10-25 出版日期:2014-06-25 发布日期:2018-09-25
通讯作者: 邓业成(1965—),男,广西全州人,广西师范大学教授,博士。E-mail: dyecheng@163.com
基金资助:
广西自然科学基金资助项目(2013GXNSFAA019058);广西高校科研资助项目(2013YB037)

Insecticidal Activities and Chemical Constituents of Essential Oils from Alien Invasive Plants Solidago canadensis and Wedelia trilobata

DENG Ye-cheng^1,2, LI Rui-yu^1,2, YANG Lin-lin^1,2, ZHONG Hui^1,2, ZHOU Qiu-yan^1,2, QING Zhen^1,2

1.Key Laboratory of Ecology of Rare and Endangered Species and Environmental ProtectionGuangxi Normal University, Ministry of Education, Guilin Guangxi 541004, China;
2. College of Life Science, Guangxi Normal University,Guilin Guangxi 541004,China

Received:2013-10-25 Online:2014-06-25 Published:2018-09-25

摘要/Abstract

摘要： 本文研究外来入侵植物加拿大一枝黄花和南美蟛蜞菊精油的杀虫活性及化学成分,结果表明,2种植物精油对3种储粮害虫有熏蒸和触杀活性。加拿大一枝黄花精油对赤拟谷盗、玉米象和绿豆象的熏蒸LC₅₀分别为7.72、237.05和43.28 μL/L,对赤拟谷盗的熏蒸毒力最高;触杀LD₅₀分别为2.71×10^-4、1.40×10^-5和2.35×10^-5 μL/头,对玉米象的触杀毒力最高。南美蟛蜞菊精油对赤拟谷盗、玉米象和绿豆象的熏蒸LC₅₀分别为187.06、156.73和0.93 μL/L,对绿豆象的熏蒸毒力最高;触杀LD₅₀分别为7.52×10^-5、2.84×10^-5和1.18×10^-5 μL/头,对绿豆象的触杀毒力最高。2种植物精油对绿豆象均有种群抑制作用,精油浓度为1~3 μL/g时,加拿大一枝黄花精油的种群抑制率为57.30%~82.12%,南美蟛蜞菊精油的种群抑制率为37.63%~67.86%。利用气相色谱-质谱联用技术对2种植物精油成分进行分析,加拿大一枝黄花精油共鉴定出58个化合物,其中大根香叶烯D是最主要成分;南美蟛蜞菊精油共鉴定出52个化合物,其中1R-α-蒎烯、D-柠檬烯和α-水芹烯是主要成分。

关键词: 加拿大一枝黄花, 南美蟛蜞菊, 精油, 杀虫活性, 化学成分

Abstract: Insecticidal activities and chemical constituents of essential oils from alien invasive plants Solidago canadensis and Wedelia trilobata were investigated. The results showed that the two plant essential oils exhibited fumigant and contact activities against three stored-product insect pests. The fumigant LC₅₀ values of essential oil of S. canadensis against Tribolium castaneum, Sitophilus zeamais and Callosobruchus chinensis were 7.72 μL/L, 237.05 μL/L and 43.28 μL/L, respectively, and the contact LD₅₀values were 2.71×10^-4 μL/adult, 1.40×10^-5 μL/adult and 2.35×10^-5 μL/adult, respectively. The fumigant LC₅₀ values of essential oil of W. trilobata against T. castaneum, S. zeamais and C. chinensis were 187.06 μL/L, 156.73 μL/L and 0.93 μL/L, respectively, and the contact LD₅₀values were 7.52×10^-5 μL/adult, 2.84×10^-5 μL/adult and 1.18×10^-5 μL/adult, respectively. The two plant essential oils also inhibited the population of C. chinensis, with the inhibition rates being 57.30%-82.12% for essential oil of S. canadensis and 37.63%-67.86% for W. trilobata at concentrations of 1-3 μL/g. Fifty eight compounds from S. canadensis oil and fifty two compounds from W. trilobata oil were identified using GC/MS technique. The most principal component of S. canadensis oil was germacrene D (49.43%), and the principal components of W. trilobata oil were 1 R-α-pinene (28.79%), D-limonene (23.70%) and α-phellandrene (10.80%).

Key words: Solidago canadensis L., Wedelia trilobata (L.) Hitchc., essential oil, insecticidal activities, chemical constituents

中图分类号:

S482.39

邓业成, 李瑞钰, 杨林林, 钟慧, 周秋艳, 卿朕. 加拿大一枝黄花和南美蟛蜞菊精油的杀虫活性及化学成分[J]. 广西师范大学学报（自然科学版）, 2014, 32(2): 122-129.

DENG Ye-cheng, LI Rui-yu, YANG Lin-lin, ZHONG Hui, ZHOU Qiu-yan, QING Zhen. Insecticidal Activities and Chemical Constituents of Essential Oils from Alien Invasive Plants Solidago canadensis and Wedelia trilobata[J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(2): 122-129.

参考文献

[1] 舒易星, 施祖荣, 王连水, 等. 外来入侵植物及其生物防治[J]. 仲恺农业工程学院学报, 2013, 26(1): 64-71.
[2] 董梅, 陆建忠, 张文驹, 等. 加拿大一枝黄花:一种正在迅速扩张的外来入侵植物[J]. 植物分类学报, 2006, 44(1): 72-85.
[3] 黄洪武, 董立尧, 李俊, 等. 外来入侵植物加拿大一枝黄花的研究进展[J]. 杂草科学, 2007 (2): 6-9.
[4] PROSSER I, ALTUG I G, PHILLIPS A L, et al. Enantiospecific (+)- and (-)-germacrene D synthases, cloned from goldenrod, reveal a functionally active variant of the universal isoprenoid-biosynthesis aspartate-rich motif[J]. Archives of Biochemistry and Biophysics, 2004, 432(2): 136-144.
[5] 张劲松, 李博, 陈家宽, 等. 加拿大一枝黄花挥发油成分及其抗菌活性[J]. 复旦学报:自然科学版, 2006, 45(3): 412-416.
[6] 王开金, 李宁, 陈列忠, 等. 加拿大一枝黄花精油的化学成分及其抗菌活性[J]. 植物资源与环境学报, 2006, 15(1): 34-36.
[7] 黄泽文, 郑庭义. 广东地区外来植物南美蟛蜞菊入侵的历史阶段与特点[J]. 广东农业科学, 2013 (4): 68-71.
[8] 吴彦琼, 胡玉佳, 廖富林. 从引进到潜在入侵的植物:南美蟛蜞菊[J]. 广西植物, 2005, 25(5): 413-418.
[9] BRITO S, CRESCENTE O, FERNáNDEZ A, et al. Efficacy of a kaurenic acid extracted from the Venezuelan plant Wedelia trilobata (Asteracea) against Leishmania (Viannia) braziliensis [J]. Biomédica: Revista del Instituto Nacional de Salud (BIOMEDICA), 2006, 26(1): 180-187.
[10] 黄雪松, 黄荣春. 大孔树脂富集三裂叶蟛蜞菊倍半萜内酯A和B的研究[J]. 中草药, 2003, 34(8): 701-704.
[11] 林碧芬, 黄志坚. 南美蟛蜞菊挥发油的抑菌活性及成分分析[J]. 中国畜牧兽医, 2011, 38(11): 69-72.
[12] 陈贤兴, 丁炳扬, 沈夕良, 等. 南美蟛蜞菊对几种经济作物的生化他感作用[J]. 甘肃科学学报, 2005, 17(4): 15-17.
[13] 欧少云. 南美蟛蜞菊毛状根乙醇提取液对种子萌发的影响[J]. 中国农学通报, 2013, 29(24): 58-61.
[14] 黄素青, 梁炳泉, 苏兆雄, 等. 植物精油的生物活性及其在有害生物控制上的应用[J]. 农药, 2010, 49(6): 397-402.
[15] TRIPATHI A K, PRAJAPATI V, VERMA N, et al. Bioactivities of the leaf essential oil of Curcuma longa (var. ch-66) on three species of stored product beeles (Coleoptera)[J]. Journal of Economic Entomology, 2002, 95(1): 183-189.
[16] KIM S, PARK C, OHH M H, et al. Contact and fumigant activities of aromatic plant extracts and essential oil against Lasioderma serricorne (Coleoptera: Anobidae)[J]. Journal of Stored Products Research, 2003, 39: 11-19.
[17] 李云寿, 邹华英, 汪禄祥, 等. 紫茎泽兰提取物对四种储粮害虫的杀虫活性[J]. 昆虫知识, 2001, 38(3): 214-216.
[18] 邓永学, 王进军, 鞠云美,等. 九种植物精油对玉米象成虫的熏蒸作用比较[J]. 农药学学报, 2004, 6(3): 85-88.
[19] 张海燕, 邓永学, 王进军. 9种植物精油对玉米象成虫驱避和种群抑制作用[J]. 植物保护学报, 2005, 32(4): 435-436.
[20] ISMAN M B. Plant essential oils for pest and disease management[J]. Crop Protection, 2000, 19: 603-608.
[21] 杨东娟, 郭守军, 陈远平, 等. 南美蟛蜞菊叶挥发油化学成分分析[J]. 西北林学院学报, 2010, 25(1): 131-134.

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