毛细管电泳-电化学发光测定人尿中的氟哌啶醇

广西师范大学学报（自然科学版） ›› 2010, Vol. 28 ›› Issue (3): 42-45.

毛细管电泳-电化学发光测定人尿中的氟哌啶醇

康艳辉^1,2, 朱廷春³, 何红梅², 邓必阳¹

1.广西师范大学化学化工学院,广西桂林541004;
2.河北工程大学医学院,河北邯郸056002;
3.邯郸学院化学系,河北邯郸056005

收稿日期:2010-06-07 出版日期:2010-09-20 发布日期:2023-02-06
通讯作者: 邓必阳(1962—),男,湖南祁阳人,广西师范大学教授,博士。E-mail:dengby16@163.com
基金资助:
国家自然科学基金资助项目(20965001);广西自然科学基金资助项目(2010GXNSFA013051)

Determination of Haloperidol in Human Urine by Capillary Electrophoresis Electrochemiluminescence

KANG Yan-hui^1,2, ZHU Ting-chun³, HE Hong-mei², DENG Bi-yang¹

1. College of Chemistry and Chemical Engineering,Guangxi NormalUniversity,Guilin Guangxi 541004;
2. College of Medical,Hebei University of Engineering,Handan Hebei 056002,China;
3. Department of Chemistry,Handan College,Handan Hebei 056005,China

Received:2010-06-07 Online:2010-09-20 Published:2023-02-06

摘要/Abstract

摘要： 建立毛细管电泳电化学发光法测定氟哌啶醇的新方法。考察了工作电极电位、磷酸盐缓冲液浓度及其pH值等对氟哌啶醇测定的影响。在优化实验条件下,氟哌啶醇浓度线性范围为0.01～10 mg·L^-1,检出限(3σ)为3.5 μg·L^-1;相对标准偏差为1.6%(3.0 mg·L^-1,n=11)。利用该法测定了人尿加标后氟哌啶醇的含量,回收率为95.0%～99.5%。

关键词: 毛细管电泳, 电化学发光, 氟哌啶醇, 钌联吡啶

Abstract: A novel method of capillary electrophoresis (CE) withtris(2,2′-bipyridyl)ruthenium(Ⅱ) electrochemiluminescence (ECL) detection has been developed for the determination of haloperidol in human urine.The parameters such as workelectrode potential,concentration and pH of phosphate buffer solution on effectof ECL intensity of haloperidol were investigated.Under optimized conditions,haloperidol concentration was linear with ECL peak area in the range of 0.01～10mg·L^-1.The detection limit (3σ) for haloperidol was 3.5 μg·L^-1,and the RSD was 1.6% for 3.0 mg·L^-1 haloperidol (n=11).The recoveries were between 95.0% and 99.5%.

Key words: capillary electrophoresis, electrochemiluminescence, haloperidol, tris(2,2′-bipyridyl)ruthenium(Ⅱ)

中图分类号:

O657.8

康艳辉, 朱廷春, 何红梅, 邓必阳. 毛细管电泳-电化学发光测定人尿中的氟哌啶醇[J]. 广西师范大学学报（自然科学版）, 2010, 28(3): 42-45.

KANG Yan-hui, ZHU Ting-chun, HE Hong-mei, DENG Bi-yang. Determination of Haloperidol in Human Urine by Capillary Electrophoresis Electrochemiluminescence[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(3): 42-45.

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