广西师范大学学报（自然科学版） ›› 2010, Vol. 28 ›› Issue (3): 37-41.

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HPLC-共振瑞利散射联用测定庆大霉素各组分

刘承伟1, 罗志辉2,3,4, 陆尉天1, 于兰1, 卢昕2,3, 赵书林2,3   

  1. 1.桂林医学院基础医学院,广西桂林541004;
    2.广西师范大学药用资源化学与药物分子工程教育部重点实验室,广西桂林541004;
    3.广西师范大学化学化工学院,广西桂林541004;
    4.玉林师范学院化学与生物系,广西玉林537000
  • 收稿日期:2010-05-27 出版日期:2010-09-20 发布日期:2023-02-06
  • 通讯作者: 卢昕(1962—),女,安徽怀宁人,广西师范大学教授。E-mail:luxin-chem@163.com
  • 基金资助:
    国家自然科学基金资助项目(20665002);广西自然科学基金资助项目(桂科自0991094)

Development of a Novel HPLC-RRS Method for Determination of Gentamicin Components

LIU Cheng-wei1, LUO Zhi-hui2,3, LU Wei-tian1, YU Lan1, LU Xin2, ZHAO Shu-lin2   

  1. 1. College of Basic Medicine,Guilin Medical University,GuilinGuangxi 541004,China;
    2. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Ministry of Education, Guangxi Normal University,Guilin Guangxi 541004,China;
    3. College of Chemistry and Chemical Engineering, Guangxi Normal University,Guilin Guangxi 541004,China;
    4. Department of Chemistry and Biology, Yulin Normal University,Yulin Guangxi 537000,China
  • Received:2010-05-27 Online:2010-09-20 Published:2023-02-06

摘要： 建立无需衍生的高效液相色谱-共振瑞利散射联用测定庆大霉素(gentamicin,Gen)C组分的方法。滂胺天蓝作为分子识别探针,与Gen生成离子缔合物,产生增强的散射信号,荧光检测器设定λex=λem=356 nm进行检测。实验结果表明:Gen中C1、C1a、C2和C2a4个主要组分的浓度分别在7.2～115.2、8.2～131.6、7.5～78.8、8.5～78.0 mg/L具有良好线性关系,检出限在6.0～7.2 mg/L。将方法应用于临床使用的Gen注射液测定,结果符合《中国药典》2005版规定的范围。

关键词: 高效液相色谱, 共振瑞利散射, 庆大霉素, 滂胺天蓝

Abstract: A selective and rapid high-performance liquid chromatography coupled with resonance Rayleigh scattering detection method was developed for the determination of multicomponent gentamicin (Gen).Pontamine sky blue was chosen as molecular recognition probe,which could forms ion-association complex with Gen inmobile medium and produce strong scattering signal.The separation of Gen components was achieved on a C18 column and the column eluent was monitored usingfluorescence detection at λex=λem=356 nm.The detection limitsof 6.0～7.2 mg/L were reached,and the response linearity between peak area and components concentration were found in the range of 7.2～115,8.2～132,7.5～79 and 8.5～78 mg/L for C1,C1a,C2,C2a,respectively.This method has been applied to the determination of Gen components in commercial Gen sulfate injection,and the detection results were in good agreement with the specification of Chinese Pharmacopoeia.

Key words: high-performance liquid chromatography, resonance Rayleigh scattering, gentamicin, pontamine sky blue

中图分类号: 

  • O652.63
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