广西师范大学学报（自然科学版） ›› 2019, Vol. 37 ›› Issue (2): 158-167.doi: 10.16088/j.issn.1001-6600.2019.02.020

• • 上一篇    下一篇

岩性与植被类型对喀斯特土壤AM真菌群落的影响

张晓晓1,2,3, 王苗苗2,3, 冯书珍4, 邱虎森2,3, 盖爽爽5, 赵蕾1,2,3, 胡亚军2,3, 何寻阳2,3*, 陆祖军1   

  1. 1.广西师范大学生命科学学院,广西桂林541006;
    2.中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室, 湖南长沙410125;
    3.中国科学院环江喀斯特生态系统观测研究站,广西环江547100;
    4.广西科技大学医学院,广西柳州545006;
    5.广西科技师范学院经济与管理学院,广西柳州545004
  • 收稿日期:2018-03-01 出版日期:2019-04-25 发布日期:2019-04-28
  • 通讯作者: 何寻阳(1972—),男,湖南平江人,中国科学院亚热带农业生态研究所研究员,博士。E-mail:hbhpjhn@isa.ac.cn
  • 基金资助:
    国家重点研发计划项目(2016YFC0502404);国家973计划课题(2015CB452703)

Effects of Lithology and Vegetation Type on the Soil AM Fungi Community in Karst Region

ZHANG Xiaoxiao1,2,3, WANG Miaomiao2,3, FENG Shuzhen4, QIU Husen2,3, GAI Shuangshuang5, ZHAO Lei1,2,3, HU Yajun2,3, HE Xunyang2,3*, LU Zujun1   

  1. 1.College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    2.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha Hunan 410125, China;
    3.Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang Guangxi 547100, China;
    4.College of Medicine, Guangxi University of Science and Technology, Liuzhou Guangxi 545006, China;
    5.College of Economic and Management, Guangxi Science and Technology Normal University, Liuzhou Guangxi 545004, China
  • Received:2018-03-01 Online:2019-04-25 Published:2019-04-28

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摘要： 运用巢式PCR和高通量测序技术,检测不同岩性和植被类型条件下喀斯特土壤丛枝菌根(arbuscular mycorrhizal,AM)真菌群落结构的变化,揭示不同岩性和植被类型条件下土壤中AM真菌群落结构的影响因子。高通量测序共获得185个操作分类单元(operational taxonomic unit,OTU),这些OTU可划分为7科9属,其中球囊霉属Glomus为研究区内的优势属。单因素方差和独立样本t检验分析表明,在不同岩性和植被类型条件下,土壤理化性质、AM真菌优势属均存在显著性差异。双因素方差分析结果表明,岩性和植被类型及两者的交互作用对AM真菌多样性及群落存在显著性影响。主坐标分析结果显示,在植被恢复阶段不同岩性条件下,AM真菌群落结构存在显著性差异。冗余分析表明,土壤的全磷、全钾、速效磷、砂粒含量、交换性镁离子影响AM真菌群落组成结构。综上可见,岩性和植被改变了土壤的全磷、全钾、速效磷、砂粒含量、交换性镁离子,并因此影响了土壤AM真菌的群落结构。

关键词: 喀斯特, 岩性, 植被类型, 丛枝菌根真菌, 群落结构

Abstract: The changes of arbuscular mycorrhizal (AM) Fungi Community Structure in karst soil under different lithological and vegetation types were detected by nested PCR and high-throughput sequencing, and the influencing factors of AM Fungi Community Structure under different lithological and vegetation types were revealed.A total of 185 OTU (Operational Taxonomic Unit) were obtained from high-throughput sequencing. These OTU could be divided into 7 families and 9 genera, of which the Glomus was the dominant genus in the study area. The results of One-way ANOVA and independent sample t-test analysis showed that there were significant differences in soil physical and chemical properties and AM fungi dominant genus between sample plots under the different lithology and vegetation type conditions. The results of Two-way ANOVA showed that the lithology and vegetation type and the interaction between them had significant influence on the diversity and community of AM fungi. The results of PCoA showed that there were significant differences in the structure of AM fungi community under the different lithology and vegetation type conditions. The results of RDA showed that soil total phosphorus, total potassium, available phosphorus, sand content and exchangeable magnesium ions affected the structure of AM fungal community. In summary, the research results showed that the lithology and vegetation type changed soil total phosphorus, total potassium, available phosphorus, sand content, exchangeable magnesium ions in soil, and thus affected the structure of AM fungi community in soil.

Key words: Karst, lithology, vegetation type, arbuscular mycorrhizal fungi, community structure

中图分类号: 

  • Q938.1
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