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广西师范大学学报(自然科学版) ›› 2013, Vol. 31 ›› Issue (4): 109-114.
王开卓1,2, 宾石玉1, 李虹辉2, 李玉珑1,2, 刘知行2, 张建社2, 褚武英2
WANG Kai-zhuo1,2, BIN Shi-yu1, LI Hong-hui2, LI Yu-long2, LIU Zhi-xing2, ZHANG Jian-she2, CHU Wu-ying2
摘要: 本研究采用RT-PCR技术克隆得到翘嘴鳜Myostatin基因ORF区序列。经过生物信息学分析表明,翘嘴鳜Myostatin基因的编码区全长1 131 bp,编码376个氨基酸。序列分析结果表明,翘嘴鳜的Myostatin存在一个典型的TGF-β功能区域,而且C-末端区有生物活性保守性很高的9个半胱氨酸残基,这些氨基酸残基由二硫键的形式链接形成“Cys Knot”结构。对翘嘴鳜Myostatin进行成鱼红白肌表达分析表明其在红肌中的表达量显著高于白肌。研究结果为科学、系统地阐明经济鱼类骨骼肌纤维分型和分化调控机制奠定了基础。
中图分类号:
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