广西师范大学学报（自然科学版） ›› 2013, Vol. 31 ›› Issue (4): 109-114.

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翘嘴鳜生长抑制素基因的克隆及其表达分析

王开卓1,2, 宾石玉1, 李虹辉2, 李玉珑1,2, 刘知行2, 张建社2, 褚武英2   

  1. 1.广西师范大学生命科学学院,广西桂林541004;
    2.长沙大学生物工程与环境科学系,湖南长沙410003
  • 收稿日期:2013-06-27 出版日期:2013-12-20 发布日期:2018-11-26
  • 通讯作者: 宾石玉(1963—),男,广西全州人,广西师范大学教授,博士。E-mail:binsy@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金资助项目(31230076,31072195)

Molecular Cloning and Expression Analysis of Myostatin Gene in Mandarin Fish (Siniperca chuatsi)

WANG Kai-zhuo1,2, BIN Shi-yu1, LI Hong-hui2, LI Yu-long2, LIU Zhi-xing2, ZHANG Jian-she2, CHU Wu-ying2   

  1. 1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541004 China;
    2.College of Biological Engineering and Environmental Science,Changsha University,Changsha Hunan 410003,China
  • Received:2013-06-27 Online:2013-12-20 Published:2018-11-26

摘要： 本研究采用RT-PCR技术克隆得到翘嘴鳜Myostatin基因ORF区序列。经过生物信息学分析表明,翘嘴鳜Myostatin基因的编码区全长1 131 bp,编码376个氨基酸。序列分析结果表明,翘嘴鳜的Myostatin存在一个典型的TGF-β功能区域,而且C-末端区有生物活性保守性很高的9个半胱氨酸残基,这些氨基酸残基由二硫键的形式链接形成“Cys Knot”结构。对翘嘴鳜Myostatin进行成鱼红白肌表达分析表明其在红肌中的表达量显著高于白肌。研究结果为科学、系统地阐明经济鱼类骨骼肌纤维分型和分化调控机制奠定了基础。

关键词: 翘嘴鳜, 骨骼肌, 肌肉生长抑制素, 基因克隆, 表达分析

Abstract: In this study,the myostatin of Mandarin fish was cloned and the expression of the gene in the red and white muscle was analysed.The myostatin gene is 1 131 bp in length and encodes 376 amino acids.Sequence analysis showed that the myostatin contains a typical TGF-β function area and nine conserved cysteine residues in the C-terminal.These amino acid residues were linked by disulfide bonds to form “Cys Knot” structure.The expression level in red muscle was significantly higher than that in white muscle.The above results will make contribution to understanding the skeletal muscle regulatory mechanism of the economic fish.

Key words: Siniperca chuatsi, skeletal muscle, myostatin, gene clone, expression analysis

中图分类号: 

  • Q959.483
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