广西师范大学学报（自然科学版） ›› 2020, Vol. 38 ›› Issue (1): 102-106.doi: 10.16088/j.issn.1001-6600.2020.01.013

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HepG2细胞中SDR9C7蛋白的亚细胞定位研究

周洁1,2, 曾志鹏1,2, 李金月2, 陈俏媛1,2, 林万华1,2*   

  1. 1. 广西高校干细胞与医药生物技术重点实验室(广西师范大学),广西桂林541004;
    2. 广西师范大学生命科学学院,广西桂林541006
  • 收稿日期:2018-10-16 出版日期:2020-01-25 发布日期:2020-01-15
  • 通讯作者: 林万华(1975—),男,江西南康人,广西师范大学副教授,博士。E-mail: lwh@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金(31560248);广西自然科学基金(2016GXNSFAA380176);广西师范大学博士科研启动基金;广西“八桂学者”项目

Study on Subcellular Localization of SDR9C7 Protein in HepG2 Cells

ZHOU Jie1,2, ZENG Zhipeng1,2, LI Jinyue2, CHEN Qiaoyuan1,2, LIN Wanhua1,2*   

  1. 1. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University),Guilin Guangxi 541004,China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006,China
  • Received:2018-10-16 Online:2020-01-25 Published:2020-01-15

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摘要： 前人研究发现SDR9C7蛋白在Hela细胞内呈颗粒状分布,但却发现它并没有亚细胞器(如过氧化物酶体、早期内涵体、线粒体、内质网或细胞核)共定位。为探讨SDR9C7蛋白的亚细胞定位,本文采用细胞免疫组化、激光共聚焦显微镜观察法,分别检测SDR9C7蛋白与油酸诱导单纯性肝脂肪变性HepG2细胞模型中的脂滴、饥饿诱导的HepG2细胞自噬模型中的自噬体和正常培养的HepG2细胞中的高尔基体的共定位情况。结果表明,SDR9C7蛋白为全细胞分散分布,没有呈颗粒状与脂滴、自噬体、高尔基体的共定位分布。

关键词: SDR9C7, 脂滴, 自噬体, 高尔基体, 亚细胞定位

Abstract: Previous subcellular localization studies shows that SDR9C7 protein showed a granular pattern in Hela cells, but counterstaining experiments showed neither peroxisomal, early endosome, endoplasmic reticulum (ER), cytoplasmic, nuclear nor mitochondrial co-localized with SDR9C7 protein. In this study,the cellular immunofluorescence and confocal laser microscopy method were used to determine subcellular localization of SDR9C7 protein with lipid droplets in the oleic acid induced hepatocyte steatosis model in HepG2 cells,autophagosomes in the starvation induced cellular autophagy model in HepG2 cells and Golgi apparatus in the HepG2 cells under normal culture in vitro. However, the results showed that SDR9C7 protein distributed dispersally, and there was no granular SDR9C7 protein co-localized with the lipid droplets, autophagosomes or Golgi apparatus.

Key words: SDR9C7, lipid droplet, autophagosome, Golgi apparatus, subcellular localization

中图分类号: 

  • Q28
[1] BENGT P, YVONNE K. Classification and nomenclature of the superfamily of short-chain dehydrogenases/reductases (SDRs)[J]. Chemico-Biological Interactions, 2013, 202: 111-115.
[2] UDO C T O, SAMINA S, LARS O T, et al. Binding of amyloid beta-peptide to mitochondrial hydroxyacyl-CoA dehydrogenase (ERAB): regulation of an SDR enzyme activity with implications for apoptosis in Alzheimer’s disease[J]. FEBS Letters, 1999, 451 (3): 238-242.
[3] TANG Shanhong, GAO Liucun, BI Qian, et al. SDR9C7 promotes lymph node metastases in patients with esophageal squamous cell carcinoma[J]. PloS ONE, 2013: e52184.
[4] STEWART P M, BOULTON A,KUMAR S, et al. Cortisol metabolismin human obesity: impaired cortisone-cortisol conversion in subjects with central adiposity[J]. Journal of Clincical Endocrinology and Metabolism, 1999, 84 (3): 1022-1027.
[5] CHEN Weiguo, SONG Minsun, JOSEPH L N. SDR-O: an orphan short-chain dehydrogenase/reductase localized at mouse chromosome 10/human chromosome 12[J]. Gene, 2002, 294 (2): 141-146.
[6] KOWALIK D, HALLER F, ADAMSKI J, et al. In search for function of two human orphan SDR enzymes: hydroxysteroid dehydrogenase like 2 (HSDL2) and short chain dehydrogenase/reductase orphan (SDRO)[J]. Journal of Steroid Biochemistry and Molecular Biology, 2009, 117 (4/5): 117-124.
[7] WELTE M A, GOULD A P. Lipid droplet functions beyond energy storage[J]. Biochimica et Biophysica Acta, 2017, 1862 (10): 1260-1272.
[8] 董丽红,张瑞芬,黄菲,等. 油酸诱导单纯性肝脂肪变性细胞模型的建立及应用[J]. 中国药理学通报, 2017, 33(11) : 1622-1626.
[9] 兰佳欣,乔辉,付常振,等. 7-酮基胆甾醇-9-羧基壬烷通过 SREBP1c 通路缓解油酸诱导 HepG2 细胞脂质生成[J]. 中国生物化学与分子生物学报, 2018, 34 (11): 1239-1247.
[10]陈力,王丽君,谭耀宗,等. 甜菜碱对脂肪变性HepG2细胞 Hcy、SAM /SAH 及脂代谢相关基因 mRNA 表达的影响[J]. 华南预防医学, 2013, 39 (3): 1-6.
[11]殷继明,刘凯,温韬,等. 饥饿诱导DRAM介导的自噬与HepG2和Hep3B细胞凋亡的关系[J]. 北京医学, 2013, 35 (6): 437-439.
[12]QUAN Min, LIU Shunai, WANG Qi, et al. NS5ATP9 Promotes Beclin 1-Dependent Starvation-Induced Autophagy of Hepatoblastoma Cells[J]. Journal of Cellular Biochemistry, 2015, 116 (8): 1574-1582.
[13]SHIGEHARA Y, OKUDA S, NEMER G, et al. Mutations in SDR9C7 gene encoding an enzyme for vitamin A metabolism underlies autosomal recessive congenital ichthyosis[J]. Human Molecular Genetics, 2016, 25(20): 4484-4493.
[14]TAKEICHI T, NOMURA T, TAKAMA H, et al. Deficient stratum corneum intercellular lipid in a Japanese patient with lamellar ichthyosis by a homozygous deletion mutation in SDR9C7[J]. British Journal of Dermatology, 2017, 177 (3): e62-e64.
[15]KARIM N, DURBIN-JOHNSON B, ROCKE D M, et al. Proteomic manifestations of genetic defects in autosomal recessive congenital ichthyosis[J]. Journal of Proteomics, 2019, 201: 104-109.
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