非内吞依赖型生物大分子药物胞质递送策略研究进展

doi:10.16088/j.issn.1001-6600.2023042406

摘要/Abstract

摘要： 生物大分子药物由于具有高效、高特异性等优点,已成为新一代治疗药物的重要组成部分,但其分子稳定性差、易被酶解、难以跨越生物膜。传统的生物大分子药物纳米递送策略的有效性主要受限于溶酶体逃逸效率低,针对性开发非内吞依赖型的胞质直接递送策略具有重要意义。本文综述细胞穿透肽、低pH插入肽、清道夫受体介导的非内吞作用、膜融合、内质网途径、硫醇介导、基于液-液相分离技术的非内体捕获型生物大分子药物胞内递送策略的效应机制和研究进展,并分析了技术转化难点。

关键词: 生物大分子药物, 细胞穿透肽, 巯醇, 入胞, 内质网途径, 膜融合

Abstract: Biomacromolecular drugs have become an important component of the new generation of therapeutic drugs due to their high efficiency and specificity. However, their molecular stability are poor, and they are prone to enzymatic hydrolysis, and are difficult to cross biofilms. The effectiveness of traditional nano delivery strategies for biomacromolecule drugs is mainly limited by the low escape efficiency of lysosome. It is of great significance to develop endocytosis independent direct cytoplasmic delivery strategies. This paper reviews the effect mechanism and research progress of cell penetrating peptide, low pH insertion peptide, scavenger receptor mediated non endocytosis, membrane fusion, endoplasmic reticulum pathway, mercaptan mediated, non endosome capture based intracellular delivery strategy of biomacromolecule drugs based on liquid-liquid phase separation technology, and analyzes the difficulties in technology transformation.

Key words: biomacromolecular drugs, cell penetrating peptide, mercaptol mediated entry into cells, endoplasmic reticulum pathway, membrane fusion

中图分类号: R943

袁静静, 郑宇钊, 徐晨枫, 殷婷婕. 非内吞依赖型生物大分子药物胞质递送策略研究进展[J]. 广西师范大学学报（自然科学版）, 2024, 42(1): 1-8.

YUAN Jingjing, ZHENG Yuzhao, XU Chenfeng, YIN Tingjie. Advances in Cytoplasmic Delivery Strategies for Non-Endocytosis-Dependent Biomolecules[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(1): 1-8.

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