广西师范大学学报（自然科学版） ›› 2024, Vol. 42 ›› Issue (3): 189-197.doi: 10.16088/j.issn.1001-6600.2023013001

• 研究论文 • 上一篇    下一篇

Nr4a3对乳腺癌细胞增殖、凋亡及侵袭的影响

郑婉华1,2, 莫斯萍1, 周祖平1,2,3, 蒲仕明1,2,3*   

  1. 1.广西师范大学 生命科学学院, 广西 桂林 541006;
    2.广西高校干细胞与医药生物技术重点实验室(广西师范大学), 广西 桂林 541004;
    3.广西师范大学 生物医学研究中心, 广西 桂林 541004
  • 收稿日期:2023-01-30 修回日期:2023-05-23 发布日期:2024-05-31
  • 通讯作者: 蒲仕明(1987—), 男, 四川南充人, 广西师范大学助理研究员。E-mail: pushiming77@163.com
  • 基金资助:
    国家自然科学基金(81972700, 61827819, 32260188); 广西自然科学基金(2018GXNSFBA281115); 广西师范大学大学生创新项目(S202310602001)

Knockdown of Nr4a3 in Proliferative and Apoptotic Activity of Breast Cancer Cells

ZHENG Wanhua1,2, MO Siping1, ZHOU Zuping1,2,3, PU Shiming1,2,3*   

  1. 1. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541004, China;
    3. Biomedical Research Center of Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2023-01-30 Revised:2023-05-23 Published:2024-05-31

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摘要： Nr4a3(nuclear receptor subfamily 4 group a member 3)属于核受体亚家族成员,参与细胞增殖、细胞迁移和细胞凋亡的调控。据报道,Nr4a3在多种肿瘤组织中表达下调。为探索Nr4a3在乳腺癌发生、发展中的作用,本文利用公共数据库分析NR4A3在乳腺癌组织中的表达以及与乳腺癌患者总生存率的关系。在4T1细胞系上敲除Nr4a3基因,通过流式细胞术等检测细胞凋亡、细胞周期;CCK-8与集落形成实验检测细胞活力;细胞划痕与侵袭检测细胞迁移与侵袭能力;通过q-PCR检测细胞周期和凋亡基因的表达水平。结果表明:Nr4a3在乳腺癌组织中表达水平显著下调;与乳腺癌患者的总生存率呈正相关关系;Nr4a3敲除后,细胞增殖增强,细胞凋亡减少及细胞迁移能力和侵袭能力增强。本研究揭示了Nr4a3在乳腺癌细胞增殖、凋亡、迁移和侵袭的潜在作用,可能成为乳腺癌潜在的治疗靶点。

关键词: Nr4a3, 乳腺癌, 细胞凋亡, 免疫治疗

Abstract: Nr4a3 (nuclear receptor subfamily 4 group a member 3) belongs to the nuclear receptor subfamily and can regulate genes involved in proliferation, cell migration and apoptosis. Nr4a3 has been reported to be down-regulated in a variety of cancer cells. To explore the relationship between the expression of Nr4a3 in breast cancer tissues and the clinical prognosis of breast cancer patients, and its effect on cell proliferation and apoptosis, the expression of Nr4a3 in breast cancer tissues and its relationship with overall survival were analyzed using public databases. In 4T1 cell line, apoptosis and cell cycle were detected by flow cytometry, CCK-8 and colony formation were used to detect cell viability, cell scratch and invasion persisted to measure cell migration and invasion ability, the expression levels of cell cycle and apoptosis genes were detected by q-PCR. The results showed that the expression level of Nr4a3 was down-regulated in breast cancer tissues, which was positively correlated with the overall survival rate and recurrence-free survival rate of breast cancer patients. After Nr4a3 was knockdowned, cell proliferation was enhanced, cell apoptosis was reduced, and cell migration and invasion abilities were improved. This study reveals the potential role of Nr4a3 in breast cancer cell proliferation, apoptosis, migration and invasion, and may become a potential therapeutic target for breast cancer.

Key words: Nr4a3, breast cancer, apoptosis, immunotherapy

中图分类号:  R737.9

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