克氏原螯虾8种代谢物对其引诱效果研究

doi:10.16088/j.issn.1001-6600.2022091802

广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (5): 143-151.doi: 10.16088/j.issn.1001-6600.2022091802

克氏原螯虾8种代谢物对其引诱效果研究

莫丽丽^1,2,3, 周子豪^1,2,3, 李定宏³, 曾文龙³, 武正军^1,2,3, 黄锦龙^1,2,3,4*, 孙涛^3*

1.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学),广西桂林 541006;
2.广西珍稀濒危动物生态学重点实验室(广西师范大学),广西桂林 541006;
3.广西师范大学生命科学学院,广西桂林 541006;
4.广西漓江流域景观资源保育与可持续利用重点实验室(广西师范大学),广西桂林 541006

收稿日期:2022-09-18 修回日期:2022-11-16 发布日期:2023-10-09
通讯作者: 黄锦龙(1986—),男,广西东兴人,广西师范大学副教授,博士。E-mail:jl_huang@163.com; 孙涛(1984—),女, 河北唐山人,广西师范大学讲师。E-mail:suntao9658@126.com
基金资助:
国家自然科学基金(31960280);广西科技基地和人才专项(桂科AD20159042);广西自然科学基金(2018GXNSFAA294024);广西高校中青年教师科研基础能力提升项目(2019KY0055)

Research on Luring Effect of 8 Metabolites on Procambarus clarkii

MO Lili^1,2,3, ZHOU Zihao^1,2,3, LI Dinghong³, ZENG Wenlong³, WU Zhengjun^1,2,3, HUANG Jinlong^1,2,3,4*, SUN Tao^3*

1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
2. Guangxi Key Laboratory of Rare and Endangered Animal Ecology (Guangxi Normal University), Guilin Guangxi 541006, China;
3. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
4. Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), Guilin Guangxi 541006, China

Received:2022-09-18 Revised:2022-11-16 Published:2023-10-09

摘要/Abstract

摘要： 克氏原螯虾Procambarus clarkii能够识别水中信息素进行求偶,明确其信息素的有效组分能为克氏原螯虾绿色治理提供理论依据。本文采用Y型水迷宫测试鸟苷-5′-单磷酸二钠、鸟苷-5′-二磷酸二钠、鸟苷-5′-三磷酸二钠、N-乙酰-L-亮氨酸、己酸孕酮、乙炔雌二醇、胆红素和4-巯基尿苷等8种化合物对克氏原螯虾选择行为的影响,筛选具有引诱作用的乙炔雌二醇和己酸孕酮进行浓度测试。结果表明:雄性克氏原螯虾对4-巯基尿苷、胆红素、N-乙酰-L-亮氨酸、鸟苷-5′-三磷酸二钠、鸟苷-5′-二磷酸二钠、鸟苷-5′-单磷酸二钠的选择反应率接近50%,选择系数在±0.15之间,引诱效果不显著(P>0.05);对乙炔雌二醇和己酸孕酮选择反应率高,分别为64.10%、63.24%,选择系数为0.28、0.26,且表现出性别上的显著差异。对雄虾,乙炔雌二醇的最低有效引诱阈值是10 μmol/L,己酸孕酮的最低有效引诱阈值是100 μmol/L,而雌虾对这2种化合物不敏感。因此,己酸孕酮和乙炔雌二醇对雄性克氏原螯虾具有明显的引诱效果,却对雌鳌虾无明显反应,表明己酸孕酮和乙炔雌二醇是克氏原螯虾性信息素的有效成分。

关键词: 性信息素, 克氏原螯虾, 己酸孕酮, 乙炔雌二醇, Y型迷宫

Abstract: Pheromones can be recognized by Procambarus clarkii in water for courtship. Clarifying the effective components of P. clarkii pheromones can provide a theoretical basis for green management of aquatic invasive P. clarkii. In this research, the effects of eight standard compounds, namely 4-thiouridine, bilirubin, ethinyl estradiol, 17α-hydroxyprogesterone caproate, N-acetyl-L-leucine, GTP disodium salt, and GDP disodium salt and GMP disodium salt, on the selection behavior of P. clarkii were tested using a Y-shaped water maze, and then screened for the eliciting effect of ethinyl estradiol and 17α-hydroxyprogesterone caproate to prepare concentration assays. The results showed that the male P. clarkii showed nearly 50% selective response rate to 4-thiouridine, bilirubin, N-acetyl-L-leucine, GTP disodium salt, and GDP disodium salt and GMP disodium salt, with selection coefficients between ±0.15 and insignificant luring effects (P>0.05 ). P. clarkii showed high selection response rates to ethinyl estradiol (64.10%) and 17α-hydroxyprogesterone caproate (63.24%) with selection coefficients of 0.28 and 0.26, which showed significant sex differences. The minimum effective luring threshold of ethinyl estradiol and 17α-hydroxyprogesterone caproate to male P. clarkii were 10 and 100 μmol/L, respectively, while female P. clarkii were not sensitive to both compounds. Thus, ethinyl estradiol and 17α-hydroxyprogesterone caproate had significant luring effects on adult male P. clarkii but no significant response to female crayfish, which tentatively showed that ethinyl estradiol and 17α-hydroxyprogesterone caproate were the active components of the sex pheromone in P. clarkii.

Key words: sex pheromone, Procambarus clakii, ethinyl estradiol, 17α-hydroxyprogesterone caproate, Y-shaped maze

中图分类号: S917.4

莫丽丽, 周子豪, 李定宏, 曾文龙, 武正军, 黄锦龙, 孙涛. 克氏原螯虾8种代谢物对其引诱效果研究[J]. 广西师范大学学报（自然科学版）, 2023, 41(5): 143-151.

MO Lili, ZHOU Zihao, LI Dinghong, ZENG Wenlong, WU Zhengjun, HUANG Jinlong, SUN Tao. Research on Luring Effect of 8 Metabolites on Procambarus clarkii[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 143-151.

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克氏原螯虾8种代谢物对其引诱效果研究

Research on Luring Effect of 8 Metabolites on Procambarus clarkii

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