广西师范大学学报（自然科学版） ›› 2021, Vol. 39 ›› Issue (5): 190-197.doi: 10.16088/j.issn.1001-6600.2020110204

• 研究论文 • 上一篇    下一篇

鳜RORα基因的胚胎发育特征及饥饿对其节律性表达影响分析

刘晶洁1, 褚武英2, 朱鑫2, 孙悦1, 许艺兰1, 谢炎东1, 宾石玉1*, 张建社2   

  1. 1.广西师范大学 生命科学学院,广西 桂林 541006;
    2.长沙学院 生物与环境工程学院,湖南 长沙 410003
  • 收稿日期:2020-11-02 修回日期:2020-11-19 出版日期:2021-09-25 发布日期:2021-10-19
  • 通讯作者: 宾石玉(1963—),男,广西全州人,广西师范大学教授,博士。E-mail: binsy@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金(31560718, 3197210054)

Embryonic Development Characteristics and Rhythmic Expression Analysis of RORα Gene under Starvation in Siniperca chuatsi

LIU Jingjie1, CHU Wuying2, ZHU Xin2, SUN Yue1, XU Yilan1, XIE Yandong1, BIN Shiyu1*, ZHANG Jianshe2   

  1. 1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Department of Biology and Environment Engineering, Changsha University, Changsha Hunan 410003, China
  • Received:2020-11-02 Revised:2020-11-19 Online:2021-09-25 Published:2021-10-19

PDF (PC)

182

摘要： 为探究鳜RORα基因的表达特征及饥饿对其节律性调控的影响,采用实时荧光定量PCR分析RORα在鳜胚胎不同发育阶段的表达,以及短期饥饿后RORα基因在鳜肌肉和肝脏中的昼夜节律表达。结果显示:RORα基因在卵裂期表达量低,囊胚早期至原肠胚早期呈现较高水平的表达,原肠胚晚期至仔鱼期表达降低;除尾芽期外,其余各阶段的表达量差异不显著。正常投喂时,鳜肌肉和肝脏中RORα基因呈现明显的昼夜节律性,均为昼高夜低的节律性振荡;而饥饿5 d后,肌肉中RORα基因昼夜节律性表达消失,而肝脏中RORα基因虽有昼夜节律性,但其中值、振荡幅度、峰值相位等影响显著(P<0.05)。实验表明:RORα基因可能对鳜胚胎的囊胚至原肠胚期起重要作用;饥饿胁迫影响RORα基因在鳜肌肉和肝脏的昼夜节律性,进而可能导致鳜的生理过程发生紊乱。

关键词: 鳜, RORα, 胚胎发育, 饥饿, 昼夜节律

Abstract: In order to explore the expression characteristics of RORα gene in Siniperca chuatsi and the effect of starvation on its rhythm, the expression of RORα in S. chuatsi different embryonic developmental stages and the circadian expression of RORα gene in muscle and liver of S. chuatsi after short-term starvation were detected by real-time quantitative PCR. The results showed that the expression of RORα gene was low in cleavage stage, higher in early blastula to early-gastrula stage, and lower in late-gastrula embryo to larval stage. There was no significant difference from late-gastrula embryo to larval stage except tail bud stage. The expression of RORα gene in muscle and liver of S. chuatsi showed obvious circadian rhythm, which was higher in the daytime and lower in the night; however, the circadian expression of RORα gene in muscle disappeared after 5 days of starvation. Although RORα gene showed circadian rhythm in liver, its median value, oscillation amplitude and peak phase had significant effects (P<0.05). In summary, RORα gene may play an important role in the blastula to early-gastrula stage of S. chuatsi. Furthermore, starvation stress could affect the circadian rhythm of RORα gene in muscle and liver of S. chuatsi, which may lead to the disorder of physiological process.

Key words: Siniperca chuatsi, RORα, embryonic development, starvation, circadian rhythm

中图分类号: 

  • S917.4
[1] 鄢慧玲, 褚武英, 吴萍, 等. 鳜肝脏和心脏生物钟基因表达节律分析[J]. 水生生物学报, 2016, 40(2): 243-251. DOI:10.7541/2016.34.
[2] BARNEA M, MADAR Z, FROY O. High-fat diet delays and fasting advances the circadian expression of adiponectin signaling components in mouse liver[J]. Endocrinology, 2009, 150(1): 161-168. DOI:10.1210/en.2008-0944.
[3] 张方亮, 邓小红, 朱晓彤, 等. 翘嘴鳜USP7基因cDNA的克隆及饥饿对其表达影响分析[J]. 广西师范大学学报(自然科学版), 2018, 36(2): 141-147. DOI:10.16088/j.issn.1001-6600.2018.02.020.
[4] FARAHANI S, SOLGI L, BAYAT S, et al. RAR-related orphan receptor A: One gene with multiple functions related to migraine[J]. CNS Neuroscience & Therapeutics, 2020, 26(12): 1315-1321. DOI:10.1111/cns.13453.
[5] SCHMUTZ I, RIPPERGER J A, BAERISWYL-AEBISCHER S, et al. The mammalian clock component PERIOD2 coordinates circadian output by interaction with nuclear receptors[J]. Genes & Development, 2010, 24(4): 345-357. DOI:10.1101/gad.564110.
[6] LI J C, ZOU X M. MiR-652 serves as a prognostic biomarker in gastric cancer and promotes tumor proliferation, migration, and invasion via targeting RORα[J]. Cancer Biomarkers, 2019, 26(3): 323-331. DOI:10.3233/CBM-190361.
[7] LI Z Q, ZHAO J, LIU H Y, et al. Melatonin inhibits apoptosis in mouse Leydig cells via the retinoic acid-related orphan nuclear receptor α/p53 pathway[J]. Life Sciences, 2020, 246: 117431. DOI:10.1016/j.lfs.2020.117431.
[8] FLORES M V, HALL C, JURY A, et al. The zebrafish retinoid-related orphan receptor (ror) gene family[J]. Gene Expression Patterns, 2007, 7(5): 535-543. DOI:10.1016/j.modgep.2007.02.001.
[9] SOLT L A, KOJETIN D J, BURRIS T P. The REV-ERBs and RORs: molecular links between circadian rhythms and lipid homeostasis[J]. Future Medicinal Chemistry, 2011, 3(5): 623-638. DOI:10.4155/fmc.11.9.
[10] LAU P, FITZSIMMONS R L, RAICHUR S, et al. The orphan nuclear receptor, RORα, regulates gene expression that controls lipid metabolism: staggerer (SG/SG) mice are resistant to diet-induced obesity[J]. The Journal of Biological Chemistry, 2008, 283(26): 18411-18421. DOI:10.1074/jbc.M710526200.
[11] ITOH Y, FUCHINO H, SANOSAKA M, et al. Pterosin B has multiple targets in gluconeogenic programs, including coenzyme Q in RORα-SRC2 signaling[J]. Biochemical and Biophysical Research Communications, 2016, 473(2): 415-420. DOI:10.1016/j.bbrc.2016.03.016.
[12] YANG Y, DONG F S, LIU X G, et al. Dysregulation of circadian rhythm in zebrafish (Danio rerio) by thifluzamide: Involvement of positive and negative regulators[J]. Chemosphere, 2019, 235: 280-287. DOI:10.1016/j.chemosphere.2019.06.153.
[13] TAIRA G, ONOUE T, HIKIMA J-I, et al. Circadian clock components Bmal1 and Clock1 regulate tlr9 gene expression in the Japanese medaka (Oryzias latipes)[J]. Fish & Shellfish Immunology, 2020, 105: 438-445. DOI:10.1016/j.fsi.2020.07.009.
[14] LAZADO C C, VOLDVIK V. Temporal control of responses to chemically induced oxidative stress in the gill mucosa of Atlantic salmon (Salmo salar)[J]. Journal of Photochemistry and Photobiology B: Biology, 2020, 205: 111851. DOI:10.1016/j.jphotobiol.2020.111851.
[15] MARTINS R S T, GOMEZ A, ZANUY S, et al. Photoperiodic modulation of circadian clock and reproductive axis gene expression in the pre-pubertal European sea bass brain[J]. PLoS ONE, 2015, 10(12): e144158. DOI:10.1371/journal.pone.0144158.
[16] 王开卓, 宾石玉, 李虹辉, 等. 翘嘴鳜生长抑制素基因的克隆及其表达分析[J]. 广西师范大学学报(自然科学版), 2013, 31(4): 109-114. DOI:10.16088 /j.issn.1001-6600.2013.04.017.
[17] 刘希良, 宾石玉, 王开卓, 等. 翘嘴鳜的人工繁殖与胚胎发育观察[J]. 广西师范大学学报(自然科学版), 2013, 31(2): 100-106. DOI:10.16088 /j.issn.1001-6600.2013.02.009.
[18] 徐蕾, 陈小保, 唐春, 等. 翘嘴鳜UCP3基因cDNA的克隆及其胚胎发育表达分析[J]. 广西师范大学学报(自然科学版), 2017, 35(1): 92-97. DOI:10.16088/j.ssn.1001-6600.2017.01.015.
[19] BROWN R A M, EPIS M R, HORSHAM J L, et al. Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal[J]. BMC Biotechnology, 2018, 18: 16. DOI:10.1186/s12896-018-0421-6.
[20] ZHOU R X, MENG T, MENG H B, et al. Selection of reference genes in transcription analysis of gene expression of the mandarin fish, Siniperca chuasti[J]. Zoological Research, 2010, 31(2): 141-146. DOI:10.3724/SP.J.1141.2010.02141.
[21] WU P, LI Y L, CHENG J, et al. Daily rhythmicity of clock gene transcript levels in fast and slow muscle fibers from Chinese perch (Siniperca chuatsi)[J/OL]. BMC Genomics, 2016, 17(1):1008.
[22] 胡姜丽, 徐蕾, 许艺兰, 等. 鳜Follistatin基因cDNA的克隆及其胚胎发育表达分析[J]. 广西师范大学学报(自然科学版), 2020, 38(4): 124-131. DOI:10.16088/j.issn.1001-6600.2020.04.015.
[23] 农小献, 赵发兰, 宾石玉, 等. 斑鳜轻链3基因(MLC3)cDNA的克隆及纵向表达分析[J]. 湖南师范大学自然科学学报, 2011, 34(2): 79-83.
[24] 陈圆华, 李虹辉, 王利香, 等. GSK4112对尼罗罗非鱼肝脏Nr1d1和Ulk1b基因昼夜节律性表达的影响[J/OL]. 基因组学与应用生物学, 2019: 1-9[2021-03-19]. http:// kns.cnki.net/kcms/detail/45.1369.q.20191120.1333.006.html.
[25] GOLD D A, GENT P M, HAMILTON B A. RORα in genetic control of cerebellum development: 50 staggering years[J]. Brain Research, 2007, 1140(6): 19-25. DOI:10.1016/j.brainres.2005.11.080.
[26] PARAVICINI G, STEINMAYR M, ANDRÉ E, et al. The metastasis suppressor candidate nucleotide diphosphate kinase NM23 specifically interacts with members of the ROR/RZR nuclear orphan receptor subfamily[J]. Biochemical and Biophysical Research Communications, 1996, 227(1): 82-87. DOI:10.1006/bbrc.1996.1471.
[27] 王晓龙, 温海深, 张美昭, 等. 花鲈初孵仔鱼饥饿不可逆点的确定及摄食节律研究[J]. 中国海洋大学学报(自然科学版), 2017, 47(5): 57-64. DOI:10.16441/j.cnki.hdxb.20160100.
[28] 宾石玉, 林勇, 曾兰, 等. 低温胁迫对埃及尼罗罗非鱼肝、脾和鳃细胞形态的影响[J]. 广西师范大学学报(自然科学版), 2015, 33(3): 123-128. DOI:10.16088/j.issn.1001-6600.2015.03.019.
[29] 汪建华. 短期饥饿对锦鲫肌肉和肝脏中生物钟相关基因表达的影响[D]. 桂林: 广西师范大学, 2016.
[30] 汪建华, 李玉珑, 陈敦学, 等. 饥饿再投喂对鳜肌FSRP-1、FSRP-3和肠道PepT1基因表达的影响[J]. 广西师范大学学报(自然科学版), 2016, 34(1): 144-149. DOI:10.16088/j.issn.1001-6600.2016.01.023.
[31] LUCAS-SÁNCHEZ A, ALMAIDA-PAGÁN P F, MADRID J A, et al. Age-related changes in fatty acid profile and locomotor activity rhythms in Nothobranchius korthausae[J]. Experimental Gerontology, 2011, 46(12): 970-978. DOI:10.1016/j.exger.2011.08.009.
[32] REZNICK J, PRESTON E, WILKS D L, et al. Altered feeding differentially regulates circadian rhythms and energy metabolism in liver and muscle of rats[J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 2013, 1832(1): 228-238. DOI:10.1016/j.bbadis.2012.08.010.
[33] 宾石玉, 廖芳, 杜雪松, 等. 罗非鱼耐寒性能研究进展[J]. 广西师范大学学报(自然科学版), 2021, 39(1): 10-16. DOI:10.16088/j.issn.1001-6600.2020081801.
[1] 胡姜丽, 徐蕾, 许艺兰, 刘晶洁, 孙悦, 谢炎东, 宾石玉. 鳜Follistatin基因cDNA的克隆及其胚胎发育表达分析[J]. 广西师范大学学报（自然科学版）, 2020, 38(4): 124-131.
[2] 杨丹丹, 谢永广, 宾石玉, 罗华辉, 安苗, 陈敦学. 波纹鳜线粒体基因克隆及系统发育信息分析[J]. 广西师范大学学报（自然科学版）, 2019, 37(4): 119-126.
[3] 张方亮, 邓小红, 朱晓彤, 徐蕾, 胡姜丽, 宾石玉. 翘嘴鳜USP7基因cDNA的克隆及饥饿对其表达影响分析[J]. 广西师范大学学报（自然科学版）, 2018, 36(2): 141-147.
[4] 徐蕾,陈小保,唐春,胡姜丽,宾石玉,张方亮. 翘嘴鳜UCP3基因cDNA的克隆及其胚胎发育表达分析[J]. 广西师范大学学报（自然科学版）, 2017, 35(1): 92-97.
[5] 汪建华, 李玉珑, 陈敦学, 朱鑫, 刘知行, 张建社, 褚武英, 宾石玉. 饥饿再投喂对鳜肌FSRP-1、FSRP-3和肠道PepT1基因表达的影响[J]. 广西师范大学学报（自然科学版）, 2016, 34(1): 144-149.
[6] 易潭, 刘希良, 宾石玉, 王开卓, 吴萍, 褚武英, 陈韬. 鳜鱼Siniperca chuatsi红肌sMyHC1基因cDNA的克隆及其表达分析[J]. 广西师范大学学报（自然科学版）, 2014, 32(2): 175-180.
[7] 王开卓, 宾石玉, 李虹辉, 李玉珑, 刘知行, 张建社, 褚武英. 翘嘴鳜生长抑制素基因的克隆及其表达分析[J]. 广西师范大学学报（自然科学版）, 2013, 31(4): 109-114.
[8] 刘希良, 宾石玉, 王开卓, 陈敦学, 成嘉, 张建社, 褚武英. 翘嘴鳜的人工繁殖与胚胎发育观察[J]. 广西师范大学学报（自然科学版）, 2013, 31(2): 100-106.
[9] 陈敦学, 李玉珑, 宾石玉, 农小献, 刘希良, 成嘉, 张建社, 褚武英. 翘嘴鳜线粒体基因组全序列的克隆与特征分析[J]. 广西师范大学学报（自然科学版）, 2011, 29(4): 111-116.
[10] 赵发兰, 宾石玉, 陈敦学, 农小献, 刘希良. 斑鳜肌球蛋白轻链1基因cDNA的克隆及其分析[J]. 广西师范大学学报（自然科学版）, 2011, 29(2): 99-103.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 许伦辉,尹诗德,刘易家. 基于模拟退火的自适应布谷鸟算法求解公交调度问题[J]. 广西师范大学学报（自然科学版）, 2018, 36(2): 1 -7 .
[2] 黄李, 邹艳丽, 王意, 李可. 分布式电站的3种入网方式比较研究[J]. 广西师范大学学报（自然科学版）, 2017, 35(3): 30 -36 .
[3] 陈云坤, 黎先华. Sylow子群的2-极大子群与有限群的p-幂零性[J]. 广西师范大学学报（自然科学版）, 2011, 29(2): 31 -34 .
[4] 章国凤, 于涛. Dirichlet空间上的斜Toeplitz算子[J]. 广西师范大学学报（自然科学版）, 2011, 29(2): 50 -55 .
[5] 罗辛, 潘乔, 王洪亚, 陈美, 北研二. 基于SOFM的高速图像检索算法实现[J]. 广西师范大学学报（自然科学版）, 2011, 29(2): 180 -184 .
[6] 熊顺清, 周卫红. 一种基于非采样Contourlet变换的图像水印算法[J]. 广西师范大学学报（自然科学版）, 2011, 29(2): 195 -199 .
[7] 钟祥贵, 李勇刚, 张福生, 吴勇. 自同构群阶为16p1p2…pr的有限幂零群[J]. 广西师范大学学报（自然科学版）, 2013, 31(2): 58 -63 .
[8] 花世鲜, 王迎春, 李亚军, 王恒山. 脱氢松香基12位芳基取代衍生物的合成与光学性质[J]. 广西师范大学学报（自然科学版）, 2013, 31(2): 64 -68 .
[9] 刘希良, 宾石玉, 王开卓, 陈敦学, 成嘉, 张建社, 褚武英. 翘嘴鳜的人工繁殖与胚胎发育观察[J]. 广西师范大学学报（自然科学版）, 2013, 31(2): 100 -106 .
[10] 阳丽, 孔令江. 微纳米球形颗粒之间的毛细力研究[J]. 广西师范大学学报（自然科学版）, 2012, 30(1): 1 -4 .
版权所有 © 广西师范大学学报（自然科学版）编辑部
地址：广西桂林市三里店育才路15号　邮编：541004
电话：0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发