广西师范大学学报（自然科学版） ›› 2021, Vol. 39 ›› Issue (5): 190-197.doi: 10.16088/j.issn.1001-6600.2020110204

• 研究论文 • 上一篇    下一篇

鳜RORα基因的胚胎发育特征及饥饿对其节律性表达影响分析

刘晶洁1, 褚武英2, 朱鑫2, 孙悦1, 许艺兰1, 谢炎东1, 宾石玉1*, 张建社2   

  1. 1.广西师范大学 生命科学学院,广西 桂林 541006;
    2.长沙学院 生物与环境工程学院,湖南 长沙 410003
  • 收稿日期:2020-11-02 修回日期:2020-11-19 出版日期:2021-09-25 发布日期:2021-10-19
  • 通讯作者: 宾石玉(1963—),男,广西全州人,广西师范大学教授,博士。E-mail: binsy@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金(31560718, 3197210054)

Embryonic Development Characteristics and Rhythmic Expression Analysis of RORα Gene under Starvation in Siniperca chuatsi

LIU Jingjie1, CHU Wuying2, ZHU Xin2, SUN Yue1, XU Yilan1, XIE Yandong1, BIN Shiyu1*, ZHANG Jianshe2   

  1. 1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Department of Biology and Environment Engineering, Changsha University, Changsha Hunan 410003, China
  • Received:2020-11-02 Revised:2020-11-19 Online:2021-09-25 Published:2021-10-19

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摘要： 为探究鳜RORα基因的表达特征及饥饿对其节律性调控的影响,采用实时荧光定量PCR分析RORα在鳜胚胎不同发育阶段的表达,以及短期饥饿后RORα基因在鳜肌肉和肝脏中的昼夜节律表达。结果显示:RORα基因在卵裂期表达量低,囊胚早期至原肠胚早期呈现较高水平的表达,原肠胚晚期至仔鱼期表达降低;除尾芽期外,其余各阶段的表达量差异不显著。正常投喂时,鳜肌肉和肝脏中RORα基因呈现明显的昼夜节律性,均为昼高夜低的节律性振荡;而饥饿5 d后,肌肉中RORα基因昼夜节律性表达消失,而肝脏中RORα基因虽有昼夜节律性,但其中值、振荡幅度、峰值相位等影响显著(P<0.05)。实验表明:RORα基因可能对鳜胚胎的囊胚至原肠胚期起重要作用;饥饿胁迫影响RORα基因在鳜肌肉和肝脏的昼夜节律性,进而可能导致鳜的生理过程发生紊乱。

关键词: 鳜, RORα, 胚胎发育, 饥饿, 昼夜节律

Abstract: In order to explore the expression characteristics of RORα gene in Siniperca chuatsi and the effect of starvation on its rhythm, the expression of RORα in S. chuatsi different embryonic developmental stages and the circadian expression of RORα gene in muscle and liver of S. chuatsi after short-term starvation were detected by real-time quantitative PCR. The results showed that the expression of RORα gene was low in cleavage stage, higher in early blastula to early-gastrula stage, and lower in late-gastrula embryo to larval stage. There was no significant difference from late-gastrula embryo to larval stage except tail bud stage. The expression of RORα gene in muscle and liver of S. chuatsi showed obvious circadian rhythm, which was higher in the daytime and lower in the night; however, the circadian expression of RORα gene in muscle disappeared after 5 days of starvation. Although RORα gene showed circadian rhythm in liver, its median value, oscillation amplitude and peak phase had significant effects (P<0.05). In summary, RORα gene may play an important role in the blastula to early-gastrula stage of S. chuatsi. Furthermore, starvation stress could affect the circadian rhythm of RORα gene in muscle and liver of S. chuatsi, which may lead to the disorder of physiological process.

Key words: Siniperca chuatsi, RORα, embryonic development, starvation, circadian rhythm

中图分类号: 

  • S917.4
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