广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (4): 208-219.doi: 10.16088/j.issn.1001-6600.2022062701

• 研究论文 • 上一篇    下一篇

一种快速构建小鼠肥胖模型的方法

黄力1,2, 刘泽标1,2, 朱宇1,2, 王涛1,2, 吴琼1,2*   

  1. 1.广西高校干细胞与医药生物技术重点实验室(广西师范大学),广西桂林 541004;
    2.广西师范大学生命科学学院,广西桂林 541006
  • 收稿日期:2022-06-27 修回日期:2022-10-07 出版日期:2023-07-25 发布日期:2023-09-06
  • 通讯作者: 吴琼(1984—),女,甘肃平凉人,广西师范大学副教授,博士。E-mail:wubinbin02@163.com
  • 基金资助:
    国家自然科学基金(32160170);广西自然科学基金(2020GXNSFAA259049)

A Method to Rapidly Construct a Mouse Obesity Model

HUANG Li1,2, LIU Zebiao1,2, ZHU Yu1,2, WANG Tao1,2, WU Qiong1,2*   

  1. 1. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541004, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2022-06-27 Revised:2022-10-07 Online:2023-07-25 Published:2023-09-06

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摘要： 采用高脂饮食构建小鼠肥胖模型,发现传统高脂饲料存在适口性差、建模时间长、成模率低等问题。针对这些问题,本文通过改进传统高脂饲料配方,采用间隔喂养的方法构建C57BL/6J小鼠肥胖模型。造模组成模后检测血清生化指标、脂肪质量、肝脏、脂肪、胰腺组织病理学变化。结果表明:1)投喂改进高脂饲料均能缩短造模时间,在间隔喂养方法下仅需6周即可建成小鼠肥胖模型。2)高脂饲料组血清中肝功能、肾功能相关生化指标正常,血脂升高(P<0.01);病理学组织切片显示肝组织存在轻到中度水肿,脂肪细胞体积增大,胰腺组织未见异常。本文的改进高脂饲料适口性好,能在短时间内升高小鼠体质量,比市面上同类型的高脂饲料建模时间更短且具有食品安全性。

关键词: 高脂饮食喂养, 饮食诱导肥胖, 肥胖模型, 高脂饲料, C57BL/6J小鼠

Abstract: High-fat diet was used to construct a mouse obesity model, and it was found that the traditional high-fat diet had problems such as poor palatability, long modeling time, and low molding rate. In response to these problems, the obesity model of C57BL/6J mice was established by improving the traditional high-fat diet formula and using the method of interval feeding. After modeling, serum biochemical indexes, fat weight, and histopathological changes of liver, fat and pancreas were detected. The results showed that: 1) feeding the improved high-fat diet could shorten the modeling time, and the mouse obesity model could be established in only 6 weeks under the interval feeding method; 2) the serum liver function and renal function of the high-fat diet group related to Biochemical indicators were normal, blood lipids was elevated (P<0.01), and histopathological tissue sections showed mild to moderate edema in liver tissue, increased adipocyte volume, and no abnormality in pancreatic tissue. The improved high-fat feed in this experiment had high palatability and could increase the body weight of mice in a short time, which was shorter than the same type of high-fat feed on the market and had food safety.

Key words: high-fat diet feeding, diet-induces obesity, obesity model, high-fat diet, C57BL/6J mice

中图分类号:  R589.2; R-332

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