广西师范大学学报（自然科学版） ›› 2021, Vol. 39 ›› Issue (1): 148-155.doi: 10.16088/j.issn.1001-6600.2020070901

• 研究论文 • 上一篇    下一篇

mSdr9c7基因在C57BL/6J小鼠不同组织中表达水平的研究

李银玲1,2, 周洁1,2, 周 晶1,2, 陈俏媛1,2, 曾志棚1,2, 林万华1,2*   

  1. 1.广西高校干细胞与医药生物技术重点实验室(广西师范大学), 广西 桂林 541004;
    2.广西师范大学生命科学学院, 广西 桂林 541006
  • 收稿日期:2020-07-09 修回日期:2020-08-04 出版日期:2021-01-25 发布日期:2021-03-27
  • 通讯作者: 林万华(1975—), 男, 江西南康人, 广西师范大学副教授, 博士。E-mail: lwh@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金(31560248); 广西自然科学基金(2016GXNSFAA380176)

Study on the Expression Level of mSdr9c7 Gene in Different Tissues of C57BL/6J Mice

LI Yinling 1,2, ZHOU Jie 1,2, ZHOU Jing 1,2, CHEN Qiaoyuan 1,2, ZENG Zhipeng 1,2, LIN Wanhua 1,2*   

  1. 1. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541004, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2020-07-09 Revised:2020-08-04 Online:2021-01-25 Published:2021-03-27

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摘要： 为探讨mSdr9c7基因在C57BL/6J雄性成年小鼠各组织中的表达情况,进一步研究mSdr9c7基因的功能,本文筛选适于实时荧光定量PCR法检测mSdr9c7基因表达水平的引物(正向引物序列为F:GGCCTGGACGTCAAGTTTCT,反向引物序列为R:TTCTAGAGGCCCCAGCAGAT),以5只18周龄C57BL/6J雄性小鼠为供试动物,采集其皮肤、肝脏、睾丸等16种组织,并从外周血中分离红细胞、白细胞,采用实时荧光定量PCR法检测mSdr9c7基因16种小鼠组织或细胞中mRNA的表达情况。以每只小鼠相应肝脏组织的表达量作为参照并进行统计分析,结果表明:mSdr9c7 mRNA在皮肤、肝脏、睾丸、骨髓组织为高水平表达,分别是肝脏组织的1.069±0.098 9、1.010±0.050 8、0.987±0.081 0、0.822±0.083 8倍;在血管、白细胞、大脑、血液、肾脏、肺、十二指肠、胃、外周红细胞、脾脏等组织或细胞中为中等水平表达,分别是肝脏组织的0.697±0.053 0、0.628±0.057 0、0.577±0.099 6、0.522±0.062 2、0.538±0.053 5、0.455±0.071 5、0.439±0.086 9、0.375±0.101 9、0.391±0.032 0、0.377±0.052 0倍;而在脂肪、肌肉、小脑、心脏为低水平表达,分别是肝脏组织的0.189±0.025 6、0.129±0.034 3、0.118±0.063 9、0.052±0.014 0倍。

关键词: mSdr9c7基因, C57BL/6J小鼠, 组织表达

Abstract: The expression level of mSdr9c7 gene in the tissues of C57BL/6J male adult mice was detected for further research on its function. The suitable forward primer sequence as GCCTGACGTCAAGTTCT and the backward primer sequence as TTCTAGAGGCCCCAGCAGAT for real-time fluorescence quantitative PCR were screened to detect the expression level of mSdr9c7 gene. Five 18-week-old C57BL/6J male mice were used as experimental animals. 16 kinds of tissues or cells such as skin,liver,testis,bone marrow,blood vessels,cerebellum,cerebellum,kidney,blood,lung,duodenum,spleen,stomach,fat,muscle and heart were collected,and red blood cells and lymphocyte were isolated from peripheral blood. The real-time fluorescent quantitative PCR method was used to detect the expression of mSdr9c7 gene mRNA in the above-mentioned mouse tissues or cells. The gene expression the corresponding liver tissues of each mouse was used as a reference and statistically analyzed. The results showed that mSdr9c7 mRNA was highly expressed in skin,liver,testis and bone marrow,which was 1.069±0.098 9,1.010±0.050 8,0.987±0.081 0 and 0.822±0.083 8 times as that in liver tissue respectively. It expressed at a moderate level in blood vessels,lymphocyte,brain,blood,kidney,lung,duodenum,stomach,red blood cells and spleen,and they were 0.697±0.053 0,0.628±0.057 0,0.577±0.099 6,0.522±0.062 2,0.538±0.053 5,0.455±0.071 5,0.439±0.086 9,0.375±0.101 9,0.391±0.032 0,0.377±0.052 0 times as that in liver tissue respectively. Its expression was at a low level in fat,muscle,cerebellum and heart,which was 0.189±0.025 6,0.129±0.034 3,0.118±0.063 9 and 0.052±0.014 0 times as that in liver tissue respectively.

Key words: mSdr9c7 gene, C57BL/6J mice, tissue expression

中图分类号: 

  • Q28
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[2] 唐文迪, 黎国庆, 黄俊, 黎青, 覃江克. 油茶皂素和多酚的综合提取工艺研究[J]. 广西师范大学学报（自然科学版）, 2019, 37(2): 131-138.
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