广西师范大学学报（自然科学版） ›› 2018, Vol. 36 ›› Issue (4): 90-98.doi: 10.16088/j.issn.1001-6600.2018.04.012

• • 上一篇    下一篇

微曝气-ABR处理养猪废水及微生物群落分布

卢宇翔1, 农志文1, 宿程远1,2*, 陈瑞聪1, 刘凡凡1, 黄智1, 陈孟林1   

  1. 1. 广西师范大学环境与资源学院,广西桂林541006;
    2.广西师范大学岩溶生态与环境变化研究广西高校重点实验室,广西桂林541006
  • 收稿日期:2018-01-30 发布日期:2018-10-20
  • 通讯作者: 宿程远(1981—),男,河北晋州人,广西师范大学副教授,博士。E-mail:suchengyuan2008@126.com
  • 基金资助:
    国家自然科学基金(51768009);岩溶生态与环境变化研究广西高校重点实验室资助项目(YRHJ16Z007);广西研究生教育创新计划(XYCSZ-2018066)

Efficiency and Micro-ecology of Microaeration-AnaerobicBaffled Reactor Treating Swine Wastewater

LU Yuxiang1, NONG Zhiwen1, SU Chengyuan1,2*, CHEN Ruicong1, LIU Fanfan1,
HUANG Zhi1, CHEN Menglin1   

  1. 1. School of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Key Laboratory of Karst Ecology and Environment Change of Guangxi Department of Education,Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2018-01-30 Published:2018-10-20

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摘要： 本文研究微曝气-ABR处理养猪场废水的效能,同时对ABR中厌氧污泥的脱氢酶、蛋白酶活性以及微生物群落结构进行分析。在预处理微曝气时间8 h,ABR水力停留时间24 h,并在ABR第四格室与第一格室间增加回流的条件下,整体流程COD去除率为70%～90%,氨氮的去除率为65%～75%,其中ABR对COD的去除率为60%～80%,对氨氮的去除率为40%～55%。ABR中各个格室的脱氢酶、蛋白酶的含量逐渐降低,第一格室污泥中的脱氢酶与蛋白酶的含量分别为41.06 mg/(g·h)和0.42 mg/g。高通量测序表明,ABR反应器中绿弯菌门、壁菌门、变形菌门最为丰富,特别是绿弯菌门在4个格室中的比例分别为51.12%、46.96%、62.44%和47.23%,绿弯菌门的存在有利于复杂污染物的降解;同时由Heatmap图可知,由于回流的存在,使得第四格室与第一格室的微生物群落分布更为接近。

关键词: 改良ABR, 养猪废水, 曝气, 酶活性, 微生态

Abstract: The efficiency of the microaeration-anaerobic baffled reactor (ABR) was investigated in treating swine wastewater. In addition, the activity of dehydrogenase, protease and micro-ecology in the ABR were also analyzed. At the operation conditions the pretreatment with 8 hours of micro aeration, ABR of the hydraulic retention time was 24 hours, and the adding reflux between the first compartment and the fourth compartment of the ABR, the removal efficiency of COD reached to 70%-90% in the total system, and the removal efficiency of COD was 60%-80% in the ABR. Meanwhile, the removal rate of NH3-N reached to 65%-75%, 40%-55%, respectively in the total system and ABR. The content of dehydrogenase and protease in each compartment of ABR gradually decreased, and the content of dehydrogenase and protease was 41.06 mg/(g·h) and 0.42 mg/g in the first compartment of the ABR, respectively. According to the high-throughput sequencing results, Chloroflexi, Firmicutes, Proteobacteria were dominant bacteria in the ABR sludge in particular, the abundance of Chloroflexi was 51.12%,46.96%,62.44% and 47.23% in each compartment. Chloroflexi is beneficial to the degradation of a series of complex pollutants. At the same time, known from the heatmap figure, the microbial community structures in the fourth compartment and the first compartment were much closer due to the existence of reflux.

Key words: modified anaerobic baffled reactor, swine wastewater, aeration, enzyme activity, micro-ecology

中图分类号: 

  • X703.1
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