快速荧光测定初筛高刺桐碱积累量菌株

doi:10.16088/j.issn.1001-6600.2017.03.018

广西师范大学学报（自然科学版） ›› 2017, Vol. 35 ›› Issue (3): 141-148.doi: 10.16088/j.issn.1001-6600.2017.03.018

快速荧光测定初筛高刺桐碱积累量菌株

刘祎^{1, 2}, 叶雪梅^{1, 2}, 肖咪云^{1, 2}, 吕丽君^{1, 2}, 侯澄友³, 陆祖军^{1, 2*}

1.广西师范大学生命科学学院,广西桂林541006;
2. 珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541006;
3.桂林润泰生物科技有限公司,广西桂林541001

出版日期:2017-07-25 发布日期:2018-07-25
通讯作者: 陆祖军(1963—),男(壮族),广西百色人,广西师范大学教授,博士。E-mail: luzujun2002@aliyun.com
基金资助:
国家自然科学基金(31060120);广西自然科学基金(13GXNSFAA019059);广西科技项目(桂科攻14124004-3-8,桂科攻14125006-81);广西大学生创新创业训练项目(201610602054)

The Preliminary Screening of Hypaphorine High-accumulationStrain by Using the Quick Fluorescent Method

LIU Yi^1,2,YE Xuemei^1,2,XIAO Miyun^1,2,L Lijun^1,2,HOU Chengyou³,LU Zujun^1,2*

1.College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
2.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guilin Guangxi 541006, China;
3.Guilin Runtai Biological Technology Co. LTD, Guilin Guangxi 541001, China

Online:2017-07-25 Published:2018-07-25

摘要/Abstract

摘要： 下箴刺桐碱是一种吲哚类生物碱,被认为是多种中草药的有效成分之一。彩色豆马勃Pisolithus tinctorius是一种硬皮马勃目的大型真菌,能在菌丝体内积累下箴刺桐碱。下箴刺桐碱具有比较强的天然荧光,但其荧光光谱与色氨酸几乎完全重叠,在用荧光方法筛选下箴刺桐碱高产菌株的过程中,共存的色氨酸会严重干扰下箴刺桐碱的定量。本研究构建一种色氨酸的快速荧光检测方法,运用此方法筛选出的一株彩色豆马勃菌株下箴刺桐碱含量(鲜质量含量)达到3.39 nmol·mg^-1。

关键词: 彩色豆马勃, 筛选, 下箴刺桐碱, 色氨酸, 荧光检测

Abstract: Hypaphorine is an indole alkaloid identified as one of active constituents in some Chinese medicinal herbs. Pisolithus tinctorius is a Sclerodermatales macro-fungi which accumulate hypaphorine in its hyphae. Hypaphorine’s native fluorescent radiation is strong enough,but tryptophan has almost the same fluorescence wavelength as that of Hypaphorine,which will cause serious interference when the hypaphorine was measured. A quick tryptophan-detective Fluorescent spectrometry method has been completed, and one Pisolithus tinctorius strain containing 3.39 nmol·mg^-1 hypaphorine has been screened by using this method.

Key words: Pisolithus tinctorius, screening, hypaphorine, tryptophan, fluorescent detection

中图分类号:

Q939.9

刘祎, 叶雪梅, 肖咪云, 吕丽君, 侯澄友, 陆祖军. 快速荧光测定初筛高刺桐碱积累量菌株[J]. 广西师范大学学报（自然科学版）, 2017, 35(3): 141-148.

LIU Yi,YE Xuemei,XIAO Miyun,L Lijun,HOU Chengyou,LU Zujun. The Preliminary Screening of Hypaphorine High-accumulationStrain by Using the Quick Fluorescent Method[J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(3): 141-148.

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快速荧光测定初筛高刺桐碱积累量菌株

The Preliminary Screening of Hypaphorine High-accumulationStrain by Using the Quick Fluorescent Method

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