广西师范大学学报（自然科学版） ›› 2015, Vol. 33 ›› Issue (1): 134-138.doi: 10.16088/j.issn.1001-6600.2015.01.022

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EB病毒微小RNA在鼻咽癌中的表达及意义

熊伟明1, 范才文2, 黄辉1, 王建红2, 李璐2, 向秋2, 田晶2, 雷迅1   

  1. 1.桂林医学院附属医院耳鼻咽喉头颈外科,广西桂林541001;
    2.桂林医学院生物技术学院生物化学与分子生物学教研室,广西桂林541004
  • 收稿日期:2014-09-12 出版日期:2015-03-15 发布日期:2018-09-17
  • 通讯作者: 雷迅(1956─),男,湖南长沙人,桂林医学院教授,硕导。E-mail:yypeng_7@hotmail.com
  • 基金资助:
    国家自然科学基金资助项目(81260344);广西自然科学基金资助项目(2011GXNSFD018034);广西教育厅科研项目基金资助(2013YB168);广西医学科学实验中心开放基金项目(KFJJ2010-51)

Expression and Significance of EB Virus MicroRNAin Nasopharyngeal Carcinoma

XIONG Wei-ming1, FAN Cai-wen2, HUANG Hui1, WANG Jian-hong2,
LI Lu2, XIANG Qiu2, TIAN Jing2, LEI Xun1   

  1. 1.Department of Otorhinolaryngology, Afiliated Hospital of Guilin Medical, Guilin Guangxi 541001, China;
    2.Biochemistry and Molecular Biology Teaching and Research Section, College of Biological Technology,Guilin Medical University, Guilin Guangxi 541001, China
  • Received:2014-09-12 Online:2015-03-15 Published:2018-09-17

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摘要： 采用实时荧光定量PCR分析EB病毒微小RNA(ebv-miR) ebv-miR-BART19-5p和ebv-miR-BART21-3p在鼻咽癌和鼻咽炎症组织中的表达,以便探明其表达在EB病毒(EBV)致鼻咽癌中的内在联系和意义。结果表明,鼻咽癌组织中ebv-miR-BART19-5p和ebv-miR-BART21-3p的表达水平明显高于鼻咽黏膜炎症组织。EBV可能通过ebv-miR-BART19-5p和ebv-miR-BART21-3p的表达上调介导鼻咽组织癌变,它们可能是鼻咽癌治疗的分子靶点和鼻咽癌诊断的分子标志物。

关键词: 鼻咽癌, 诊断标志物, EB病毒微小RNA

Abstract: Expression of EB virus micro RNA (ebv-miR) ebv-miR-BART19-5p and ebv-miR-BART21-3p was analyzed by real-time fluorescence quantitative PCR in nasopharyngeal carcinoma(NPC) and nasopharyngeal inflammation tissue, so as to ascertain the internal relation and significance between the expression of ebv-miRs and EB virus (EBV) induced NPC. The results showed that the expression of ebv-miR-BART19-5p and ebv-miR-BART21-3p were significantly higher in NPC tissue than that in nasopharyngeal mucosa inflammation tissue. EBV may mediate carcinogenesis of nasopharyngeal epithelium through the expression of ebv-miR-BART19-5p and ebv-miR-BART21-3p, which are likely to be molecular markers and molecular targets for diagnosis and treatment of NPC.

Key words: nasopharyngeal carcinoma, diagnosis marker, ebv-miR

中图分类号: 

  • R739.6
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