广西师范大学学报（自然科学版） ›› 2015, Vol. 33 ›› Issue (1): 139-145.doi: 10.16088/j.issn.1001-6600.2015.01.023

• • 上一篇    下一篇

沙田柚S-RNase基因的克隆及序列分析

秦新民1,2, 张渝1,2, 刘玉洁1,2, 郭丹妮1,2, 李惠敏1,2   

  1. 1.广西师范大学生命科学学院,广西桂林541004;
    2.珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004
  • 收稿日期:2014-11-24 出版日期:2015-03-15 发布日期:2018-09-17
  • 通讯作者: 秦新民(1956—),男,广西灵川人,广西师范大学教授,博士。E-mail:xmqin@mailbox.gxnu.edu.cn
  • 基金资助:
    国家自然科学基金资助项目(31360477);广西教育厅资助项目项目(2013YB036)

Cloning and Sequence Analysis of S-RNase Gene fromCitrus grandis var. Shatinyu Hort

QIN Xin-min1,2, ZHANG Yu1,2, LIU Yu-jie1,2, GUO Dan-ni1,2, LI Hui-min1,2   

  1. 1.College of Life Science, Guangxi Normal University, Guilin Guangxi 541004,China;
    2. Key Laboratoryof Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2014-11-24 Online:2015-03-15 Published:2018-09-17

PDF (PC)

241

摘要： 利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序。通过差异分析得到沙田柚S-RNase 基因序列。该基因全长为1 238 bp(GenBank登录号为KP172529),开放阅读框(ORF)全长为834 bp,共编码278个氨基酸,编码的蛋白质的相对分子质量为31.402 kDa,理论等电点为5.30。S-RNase蛋白为亲水性蛋白,共有17个可能的磷酸化位点。氨基酸序列分析表明,其编码的氨基酸与柚Citrus maxima、沙糖桔Citrus reticulata和甜橙Citrus sinensis的同源性分别为99%、98%和96%。系统进化树显示沙田柚S-RNase 基因与柚、沙糖桔和甜橙亲缘关系很近,属于同一进化分支。

关键词: 沙田柚, S-RNsae 基因, 序列分析

Abstract: In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus grandis var. Shatinyu Hort were sequenced by high-throughput sequencing technology. S-RNase gene sequences of Citrus grandis var. Shatinyu Hort was obtained through different analytical method. It is 1 238 bp (GenBank accession number:KP172529) in length with an open reading frame(ORF)of 834 bp,encoding 278 amino acids with deduced molecular weight of 31.402 kDa, and theoretical pI value of 5.30. Bioinformatics analysis showed that S-RNase is a hydrophilic protein, and there are 17 phosphorylation sites within the polypeptide chain.The homology analysis of amino acid sequence indicated that the S-RNase protein shared high homology with that of Citrus maxima (99%), Citrus reticulate (98%) and Citrus sinensis (96%). Phylogenetic analysis revealed that S-RNase gene showed closer kinship with that of Citrus maxima、Citrus reticulate and Citrus sinensis,indicating that they belong to the same evolutionary branch.

Key words: Citrus grandis var. Shatinyu Hort, S-RNase gene, sequence analysis

中图分类号: 

  • Q946
[1] TAKAYAMA S,ISOGAI A.Self-incompatibility in plants[J].Annual Review of Plant Biology,2005,56:467-489.
[2] LORD E M. Adhesion and guidance in compatible pollination[J]. Journal of Experimental Botany, 2003, 54(380):47-54.
[3] SWANSON W J, VACQUIER V D. The rapid evolution of reproductive proteins[J]. Nature Review Genetics,2002,3(2):137-144.
[4] HISCOCK S J, ALLEN A M. Diverse cell signalling pathways regulate pollen-stigma interactions: the search for consensus[J]. The New Phytologist, 2008, 179(2):286-317.
[5] DE NETTANCOURT D. Incompatibility in angiosperms[J]. Sexual Plant Reproduction, 1997,10(4):185-199.
[6] SASSA H, NISHIO T, KOWYAMA Y, et al. Self-incompatibility (S) alleles of the Rosaceae encode members of a distinct class of the T2/S ribonuclease superfamily[J]. Molecular and General Genetics,1996, 250(5):547-557.
[7] TAO R, YAMANA H, SASSA H, et al. Identification of stylar RNases associated with gametophytic self-incompatibility in almond (Prunus dulcis)[J]. Plant and Cell Physiology, 1997, 38(3):304-311.
[8] XUE Yong-biao, CARPENTER R, DICKINSON H G, et al. Origin of allelic diversity in Antirrhinum S locus RNases[J]. The Plant Cell,1996, 8(5):805-814.
[9] McCLURE B A, FRANKLIN-TONG V. Gametophytic self-incompatibility: understanding the cellular mechanisms involved in “self” pollen tube inhibition[J]. Planta, 2006, 224(2):233-245.
[10] FRANKLIN-TONG V E, RIDE J P, READ N D, et al. The self-incompatibility response in Papaver rhoeas is mediated by cytosolic free calcium[J]. The Plant Journal,1993, 4(1):163-177.
[11] FRANKLIN-TONG V E, RIDE J P, FRANKLIN F C H. Recombinant stigmatic self-incompatibility (S-) protein elicits a Ca2+ transient in pollen of Papaver rhoeas[J]. The Plant Journal, 1995, 8(2):299-307.
[12] CLARKE S R, STAIGER C J, GIBBON B C, et al. A potential signaling role for profilin in pollen of Papaver rhoeas[J]. Plant Cell, 1998, 10(6):967-979.
[13] JORDAN N D, RIDE J P, RUDD J J, et al. Inhibition of self-incompatible pollen in Papaver rhoeas involves a complex series of cellular events[J]. Annals of Botany, 2000, 85:197-202.
[14] 薛妙男,陈腾土,杨继华. 沙田柚自交和异交亲和性观察[J].园艺学报,1995,22(2):127-132.
[15] 杨继华,李红艳,薛妙男. 沙田柚花柱S-糖蛋白分离及鉴定[J].广西师范大学学报:自然科学版,2000, 18(4):66-70.
[16] 杨继华,尧桂荣,薛妙男.沙田柚花柱S-糖蛋白的纯化和N-端序列测定[J].广西师范大学学报:自然科学版,2001,19(1):72-79.
[17] 秦新民,李惠敏,薛妙男,等. 沙田柚自交、异交花粉管蛋白双向电泳分析[J].广西植物,2004,24(6):566-569.
[18] 薛妙男,李义平,张杏辉,等. 沙田柚自交花柱中识别蛋白的免疫金定位[J].园艺学报,2001,28(1):59-61.
[19] 秦新民,莫花浓,万珊,等. 沙田柚花粉管特异蛋白的免疫细胞化学研究[J].广西师范大学学报:自然科学版,2008,26(4):113-115.
[20] 秦新民,莫花浓,石菁萍,等. 沙田柚花粉管S1-RNase免疫胶体金定位研究[J].广西农业科学,2009,40(5):483-485.
[21] BARIOLA P A, HOWARD C J, TAYLOR C B, et al. The Arabidopsis ribonuclease gene RNS1 is tightly controlled in response to phosphate limitation[J]. The Plant Journal, 1994,6(5):673-685.
[1] 张渝, 刘玉洁, 郭丹妮, 覃信梅, 韩愈, 李惠敏, 秦新民. 沙田柚WRKY转录因子的克隆与序列分析[J]. 广西师范大学学报（自然科学版）, 2016, 34(3): 131-137.
[2] 易潭, 刘希良, 宾石玉, 王开卓, 吴萍, 褚武英, 陈韬. 鳜鱼Siniperca chuatsi红肌sMyHC1基因cDNA的克隆及其表达分析[J]. 广西师范大学学报（自然科学版）, 2014, 32(2): 175-180.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 广西师范大学学报（自然科学版）编辑部
地址：广西桂林市三里店育才路15号　邮编：541004
电话：0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发