广西师范大学学报（自然科学版） ›› 2015, Vol. 33 ›› Issue (1): 139-145.doi: 10.16088/j.issn.1001-6600.2015.01.023

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沙田柚S-RNase基因的克隆及序列分析

秦新民1,2, 张渝1,2, 刘玉洁1,2, 郭丹妮1,2, 李惠敏1,2   

  1. 1.广西师范大学生命科学学院,广西桂林541004;
    2.珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004
  • 收稿日期:2014-11-24 出版日期:2015-03-15 发布日期:2018-09-17
  • 通讯作者: 秦新民(1956—),男,广西灵川人,广西师范大学教授,博士。E-mail:xmqin@mailbox.gxnu.edu.cn
  • 基金资助:
    国家自然科学基金资助项目(31360477);广西教育厅资助项目项目(2013YB036)

Cloning and Sequence Analysis of S-RNase Gene fromCitrus grandis var. Shatinyu Hort

QIN Xin-min1,2, ZHANG Yu1,2, LIU Yu-jie1,2, GUO Dan-ni1,2, LI Hui-min1,2   

  1. 1.College of Life Science, Guangxi Normal University, Guilin Guangxi 541004,China;
    2. Key Laboratoryof Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2014-11-24 Online:2015-03-15 Published:2018-09-17

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摘要： 利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序。通过差异分析得到沙田柚S-RNase 基因序列。该基因全长为1 238 bp(GenBank登录号为KP172529),开放阅读框(ORF)全长为834 bp,共编码278个氨基酸,编码的蛋白质的相对分子质量为31.402 kDa,理论等电点为5.30。S-RNase蛋白为亲水性蛋白,共有17个可能的磷酸化位点。氨基酸序列分析表明,其编码的氨基酸与柚Citrus maxima、沙糖桔Citrus reticulata和甜橙Citrus sinensis的同源性分别为99%、98%和96%。系统进化树显示沙田柚S-RNase 基因与柚、沙糖桔和甜橙亲缘关系很近,属于同一进化分支。

关键词: 沙田柚, S-RNsae 基因, 序列分析

Abstract: In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus grandis var. Shatinyu Hort were sequenced by high-throughput sequencing technology. S-RNase gene sequences of Citrus grandis var. Shatinyu Hort was obtained through different analytical method. It is 1 238 bp (GenBank accession number:KP172529) in length with an open reading frame(ORF)of 834 bp,encoding 278 amino acids with deduced molecular weight of 31.402 kDa, and theoretical pI value of 5.30. Bioinformatics analysis showed that S-RNase is a hydrophilic protein, and there are 17 phosphorylation sites within the polypeptide chain.The homology analysis of amino acid sequence indicated that the S-RNase protein shared high homology with that of Citrus maxima (99%), Citrus reticulate (98%) and Citrus sinensis (96%). Phylogenetic analysis revealed that S-RNase gene showed closer kinship with that of Citrus maxima、Citrus reticulate and Citrus sinensis,indicating that they belong to the same evolutionary branch.

Key words: Citrus grandis var. Shatinyu Hort, S-RNase gene, sequence analysis

中图分类号: 

  • Q946
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