广西师范大学学报（自然科学版） ›› 2015, Vol. 33 ›› Issue (1): 127-133.doi: 10.16088/j.issn.1001-6600.2015.01.021

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广西三城市HIV-1型流行毒株gag基因亚型分析

孔衍琳1, 张帅1, 王雪雯1, 李赫伟1, 胡婷婷1, 蒋骞1, 蒋就喜1, 潘定权2, 张振开2, 李林3, 孙常荣3, 李敬云3, 梁浩4, 梁冰玉4   

  1. 1.桂林医学院附属医院感染性疾病科,广西桂林541001;
    2.桂林市疾病预防与控制中心,广西桂林541001;
    3.军事医学科学院微生物流行病研究所,北京100071;
    4.广西医科大学公共卫生学院,广西南宁530021
  • 收稿日期:2014-10-12 出版日期:2015-03-15 发布日期:2018-09-17
  • 通讯作者: 蒋就喜(1955—),男,广西桂林人,桂林医学院教授,硕导。E-mail:gljiangjx@sohu.com
  • 基金资助:
    国家自然科学基金资助项目(81273137);国家传染病重大专项资助项目(2012ZX10001-002);广西本级技术研究与开发项目(桂财教[2012]98号);桂林市科学研究与技术开发项目(20130120-18);广西壮族自治区研究生教育创新计划资助项目(YCSZ2013101)

Epidemiology and Gene Analysis of HIV-1 in Guangxi,China

KONG Yan-lin1, ZHANG Shuai1, WANG Xue-wen1, LI He-wei1, HU Ting-ting1, JIANG Qian1,
JIANG Jiu-xi1, PAN Ding-quan2, ZHANG Zhen-kai2, LI Lin3, SUN Chang-rong3, LI Jing-yun3,
LIANG Hao4, LIANG Bing-yu4   

  1. 1.Infectious Diseases Department of Affiliated Hospital of Guilin Medical University, Guangxi Guilin 541001, China;
    2.Guilin Centre for Disease Control and Prevention, Guangxi Guilin 541001, China;
    3.Epidemiology and Microbiology Institute of Military Medical Science Academy of the PLA, Beijing 100071, China;
    4. School of Public Health of Guangxi Medical University, Guangxi Nanning 530021, China
  • Received:2014-10-12 Online:2015-03-15 Published:2018-09-17

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摘要： 为了解广西近年确诊的HIV-1感染者流行病学及HIV-1亚型分布情况,分别在桂林、来宾、钦州采取样本100例、102例和103例,收集流行病学资料、采集抗凝全血、提取病毒RNA、用RT-PCR进行gag基因扩增并对产物进行测序和序列分析。结果显示:在305例样本中,男女性别比1.99∶1;青壮年年龄组患者占56.72%,>50岁年龄组患者构成比例为44.62%;职业为农民的患者比例为50.82%;初中及以下文化水平患者占76.39%;经异性性接触途径传播构成比为95.74%。最终成功扩增得到155份样本的gag基因序列,共发现4种亚型:CRF01-AE亚型 124例、CRF07-BC亚型12例、CRF08-BC亚型14例、B亚型5例。

关键词: HIV-1, 流行特征, 亚型, gag基因

Abstract: To determine the epidemiological situation of confirmed HIV-1 infections in Guangxi after 2008,and to understand the distribution of HIV-1 subtypes of Guangxi. An epidemiological survey was made on the HIV-1 infections confirmed after 2008. Among them, 100 come from Guilin, 102 from Laibin, and 103 from Qinzhou. HIV-1 RNA was extracted from their whole blood and amplified by RT-PCR. HIV-1 gag gene was sequenced directly from the PCR product.The results show that there are more men than women in the chosen patients, male to female ratio was 1.99:1.Patients aged ≥20 and ≤49 years old accounted for 56.72% in total, and patients aged 50 years old or older accounted for 44.62%. Farmers accounted for 50.82%.Patients with education level of junior school or below accounted for 76.39%. The patients′s proportion who were transmitted through heterosexual contact is 95.74%. 155 specimens are gained in 305 specimens(amplification rate of 50.82%), then sequence analysis showed that there were 4 HIV-1 subtypes, CRF01-AE(124 samples),B(5 samples),CRF-07BC(12 sample) and CRF-08BC(14 sample).

Key words: HIV-1, epidemiological characteristics, subtype, gag gene

中图分类号: 

  • R512.91
[1] 中华人民共和国卫生部,UNAIDS,WHO.中国艾滋病防治工作进展(2013)[EB/OL].[2013-11-11]. http://www. chinaaids. org.cn.
[2] 杨艳. 广西第二轮全国艾滋病综合防治示范区艾滋病流行趋势分析[J]. 广西医学,2014,36(8):1123-1125.
[3] 陈立力. 云南和广西HIV-1流行毒株的基因变异和分子进化研究[D]. 北京:中国人民解放军军事医学科学院,2011.
[4] 王勇,唐振柱,朱秋映,等.广西2009—2011年艾滋病流行特征分析[J].华南预防医学,2013,39(1):6-11.
[5] 简华慧.我国偏远地区艾滋病的防治研究综述[J].中国医药指南,2013,11(14):461-463.
[6] 蒋就喜,李赫伟,周方,等.桂林市HIV-1流行毒株的基因序列测定和亚型分析[J],广西师范大学学报:自然科学版,2010,28(2):91-95.
[7] 蒋就喜,李赫伟,周方,等. 桂北地区HIV-1感染者的HIV-1病毒亚型分布观察[J]. 山东医药,2010,50(22):13-15.
[8] 周方,骆安德,蒋就喜,等.来宾市艾滋病分子流行病学调查[J]. 热带医学杂志,2011,11(4):420-423.
[9] 王玉,周楚,吴兴华,等.广西50岁及以上嫖客HIV感染现况及影响因素研究[J]. 中华疾病控制杂志,2014,18(3):213-216.
[10] 王福祥,孙永涛,王临旭,等.中国株HIV-1核心蛋白真核表达载体的构建与表达[J].中国病毒学,2003,18(5):420-422.
[11] PETERSON PK, GEKKER G, HU S, et al. Cannabinoids and morphine differentially affect HIV-1 expression in CD4(+) lymphocyte and microglial cell cultures[J]. J Neuroimmunol, 2004, 147(1/2):123-126.
[12] ZACCARELLI M, BARRACCHINI A, De LONGIS P, et al. Factors felated to virologic failure among HIV-positive injecting drug users treated with combination antiretroviral trerapy including two nucleoside reverse transcriptase inhibitors and nevirapine[J]. AIDS Patient Care, STDS, 2002, 16(2):67-73.
[13] MUYLDERMANS G, SASSE A. HIV-1 resistance and trend analysis in newly diagnosed patients in Belgium[J]. Acta Clin Belg, 2014, 69(1):1-7.
[14] GAO Q, GU Z, PARNIAK M A, et al. The same mutation that encodes low-level human immunodeficiency virus type 1 resistance to 2′,3′-dideoxyinosine and 2′,3′-dideoxycytidine confers high-level resistance to the (-) enantiomer of 2′,3′-dideoxy-thiacytidine[J]. Antimicrob Agents Chemother, 1993, 37(6):1390-1392.
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