广西师范大学学报（自然科学版） ›› 2015, Vol. 33 ›› Issue (1): 122-126.doi: 10.16088/j.issn.1001-6600.2015.01.020

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EGCG棕榈酸酯体外抗HCV作用的研究

张杰1,2, 刘倩英2, 孙师2, 梁任山1,2, 宋德贵1   

  1. 1.广西师范大学生命科学学院,广西桂林541004;
    2.武汉生物技术研究院,湖北武汉430075
  • 收稿日期:2015-01-13 出版日期:2015-03-15 发布日期:2018-09-17
  • 通讯作者: 宋德贵(1954—),男,广西玉林人,广西师范大学教授。E-mail:sondegui@163.com
  • 基金资助:
    国家星火计划项目(2013GA790001);广西科学研究与技术开发计划项目(桂科重14121003-3-1)

Antiviral Effects of EGCG-P on Hepatitis C Virus in vitro

ZHANG Jie1,2, LIU Qian-ying2, SUN Shi2, LIANG Ren-shan1,2, SONG De-gui1   

  1. 1. College of Life Science, Guangxi Normal University, Guili Guangxi 541004, China;
    2.Wuhan Institute of Biotechnology, Wuhan Hubei 430075, China
  • Received:2015-01-13 Online:2015-03-15 Published:2018-09-17

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摘要： 为探讨表没食子儿茶素没食子酸棕榈酸酯(EGCG-P)抗丙型肝炎病毒(HCV)的作用,本研究采用cck-8法检测EGCG-P对Huh7.5.1细胞系的毒性作用,丙型肝炎病毒核酸定量检测试剂盒检测HCV滴度,通过实时荧光定量PCR(RT-PCR)选择合适的病毒感染复数(MOI),进而研究药物抗病毒的作用效果。结果:EGCG-P浓度为0~50 mg/L时对Huh7.5.1细胞无毒害作用,且抗病毒作用显著;当其浓度为25 mg/L时病毒表达量为阳性对照的1/6,可见EGCG-P能高效抑制HCV的增殖。

关键词: EGCG棕榈酸酯, 丙型肝炎病毒, Huh7.5.1, 实时荧光定量PCR, cck-8

Abstract: This research is to explore the antiviral effects of Epigallocatechin gallate palmitate (EGCG-P) on hepatitis c virus (HCV). To achieve this goal, the method of CCK 8 detecting EGCG-P toxic effect of Huh7.5.1 cell line, hepatitis C virus nucleic acid detection kit, and real-time PCR (RT-PCR) were used to study the role of antiviral drug effect. The study indicated that, when the EGCG-p concentration was from 0~50 mg/L, the antiviral effect was significant. When the concentration was 25 mg/L, virus expressing quantity was about 1/6 of the positive control. The result strongly support the conclusion that EGCG-P can efficiently inhibit the multiplication of HCV.

Key words: EGCG-P, HCV, Huh7.5.1, RT-PCR, cck-8

中图分类号: 

  • R373.2
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