Journal of Guangxi Normal University(Natural Science Edition) ›› 2025, Vol. 43 ›› Issue (4): 157-164.doi: 10.16088/j.issn.1001-6600.2024041401

• Ecology and Environmental Science Research • Previous Articles     Next Articles

Fluorescence Intensity and Polarization Method for Acetamiprid Detection Based on Graphene Oxide

YIN Nanzu1,2, HUANG Qian1,2, ZHAO Jingjin1,2*   

  1. 1. Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), Guilin Guangxi 541006, China;
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China
  • Received:2024-04-14 Revised:2024-06-27 Online:2025-07-05 Published:2025-07-14

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Abstract: A fluorescence intensity and polarization method for simultaneous detection of pesticide acetamiprid was established based on the fluorescence quenching effect and mass amplification effect of graphene oxide (GO). When nucleic acid signal probe which labeled with fluorescent molecule FAM attached to the GO surface, a lower fluorescence intensity and a higher polarization signal was detected. After acetamiprid binds to its aptamer, the released complementary probe was hybridized with the signal probe to move it away from graphene, and then the enhanced fluorescence intensity and the reduced polarization signal are obtained. The relationship between the fluorescence intensity or polarization signal and different concentrations of acetamiprid was examined, and the conditions such as the target recognition time, GO concentration and reaction time were optimized. Under the optimized experimental conditions, the fluorescence polarization detection method (LOD=5 nmol/L) had a lower detection limit than the fluorescence intensity method (LOD=50 nmol/L), while the fluorescence intensity method was more stable. This method showed good selectivity and achieved spiked recovery analysis of acetamiprid in celery leaves and lake water.

Key words: acetamiprid, graphene oxide, fluorescence analysis, fluorescence polarization, fluorescence quenching

CLC Number:  O657.3;X839.2;TQ450.263
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