桂林市HIV-1流行毒株的基因序列测定和亚型分析

Determination of Gene Sequence and Subtype Analysis of HIV-1 Strains in Guilin,China

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Abstract: To investigate the subtype distribution and the prevalence of sequence characteristics of HIV-1 strains in Guilin residents and to analyze the relationship between distribution of HIV-1 subtypes and transmissionroutines,blood samples from 42 HIV-1 infected individuals from Guilin residents and separated PBMC specimens were collected.DNAs were extracted and the envgene was amplified by nest-PCR.PCR products were sequenced directly and phlogenetic analyses of gag gene was performed using the MEGA3 software.Among 42 HIV-1 blood samples,30 env gene fragments were amplified and analyzed.Two HIV-1 subtypeswere identified in Guilin residents and more attention should be paid to the surveillance of HIV-1 gene variation.

Key words: HIV-1, subtype, Nest-polymerase chain reaction

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R512.91

JIANG Jiu-xi, LI He-wei, ZHOU Fang, LUO An-de, XURu, ZENG Si-en. Determination of Gene Sequence and Subtype Analysis of HIV-1 Strains in Guilin,China[J].Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(2): 91-95.

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