Journal of Guangxi Normal University(Natural Science Edition) ›› 2013, Vol. 31 ›› Issue (4): 109-114.

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Molecular Cloning and Expression Analysis of Myostatin Gene in Mandarin Fish (Siniperca chuatsi)

WANG Kai-zhuo1,2, BIN Shi-yu1, LI Hong-hui2, LI Yu-long2, LIU Zhi-xing2, ZHANG Jian-she2, CHU Wu-ying2   

  1. 1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541004 China;
    2.College of Biological Engineering and Environmental Science,Changsha University,Changsha Hunan 410003,China
  • Received:2013-06-27 Online:2013-12-20 Published:2018-11-26

Abstract: In this study,the myostatin of Mandarin fish was cloned and the expression of the gene in the red and white muscle was analysed.The myostatin gene is 1 131 bp in length and encodes 376 amino acids.Sequence analysis showed that the myostatin contains a typical TGF-β function area and nine conserved cysteine residues in the C-terminal.These amino acid residues were linked by disulfide bonds to form “Cys Knot” structure.The expression level in red muscle was significantly higher than that in white muscle.The above results will make contribution to understanding the skeletal muscle regulatory mechanism of the economic fish.

Key words: Siniperca chuatsi, skeletal muscle, myostatin, gene clone, expression analysis

CLC Number: 

  • Q959.483
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