Abstract: The extracts obtained from rhizome of Ficus pumila L. were evaluated for its yield of total flavonoids by standard sample rutin. Their α-glucosidase and acetylcholinesterase (AChE) inhibitory potency were also explored by the 96-micro-plate-based method. The result showed that the total flavonoids content of the extracts ranged from 0.8% to 7.1% and the extracts had high inhibitory activity toward α-glucosidase and AChE with IC₅₀ values are up to 0.018 g/L and 0.133 g/L respectively. A clear correlation between the total flavonoids content and α-glucosidase inhibitory activity was found.

Key words: α-glucosidase, flavonoids, acetylcholinesterase, Ficus pumila L.
薜荔Ficus pumila L.又名凉粉子,属桑科榕属植物,以野生为主,据本草纲目记载薜荔的药用和食用价值很早之前就被人们所发现。研究显示,薜荔根茎中富含多糖、黄酮类及维生素类等物质^[1],具有抗氧化^[2-3]、抗菌^[4]、抗炎、止血、镇痛、降血脂和清热解毒^[5]等作用。许多天然植物中提取的活性成分疗效要好于传统药物^[6-7],并且可以减少药物给患者带来的众多副作用,因而为今后药物研究发展提供了一个很好的方向^[8-9]。黄酮类化合物是自然界普遍存在于药用植物中的主要活性成分之一。大量研究表明黄酮类化合物具有很好的生物活性^[10-13],是新药研发的活性先导物的来源宝库。
 ,  ,  , 薜荔分为大叶薜荔和小叶薜荔2种,目前研究较多的是大叶薜荔的果实及花叶的提取物,对小叶薜荔报道较少,特别是对于小叶薜荔根茎提取物中活性成分及药用价值的研究报道更少。小叶薜荔取材方便,具有很高的药用价值,在广西百色地区有着丰富的资源^[14]。本研究对小叶薜荔根茎中的活性成分进行提取分离,通过不同极性洗脱剂进行纯化,并研究小叶薜荔的根茎提取物对α-葡萄糖苷酶以及乙酰胆碱酯酶的抑制能力,为小叶薜荔药用价值的进一步开发利用提供更为丰富的实验依据。

1 材料与方法