Journal of Guangxi Normal University(Natural Science Edition) ›› 2015, Vol. 33 ›› Issue (1): 139-145.doi: 10.16088/j.issn.1001-6600.2015.01.023

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Cloning and Sequence Analysis of S-RNase Gene fromCitrus grandis var. Shatinyu Hort

QIN Xin-min1,2, ZHANG Yu1,2, LIU Yu-jie1,2, GUO Dan-ni1,2, LI Hui-min1,2   

  1. 1.College of Life Science, Guangxi Normal University, Guilin Guangxi 541004,China;
    2. Key Laboratoryof Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2014-11-24 Online:2015-03-15 Published:2018-09-17

Abstract: In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus grandis var. Shatinyu Hort were sequenced by high-throughput sequencing technology. S-RNase gene sequences of Citrus grandis var. Shatinyu Hort was obtained through different analytical method. It is 1 238 bp (GenBank accession number:KP172529) in length with an open reading frame(ORF)of 834 bp,encoding 278 amino acids with deduced molecular weight of 31.402 kDa, and theoretical pI value of 5.30. Bioinformatics analysis showed that S-RNase is a hydrophilic protein, and there are 17 phosphorylation sites within the polypeptide chain.The homology analysis of amino acid sequence indicated that the S-RNase protein shared high homology with that of Citrus maxima (99%), Citrus reticulate (98%) and Citrus sinensis (96%). Phylogenetic analysis revealed that S-RNase gene showed closer kinship with that of Citrus maxima、Citrus reticulate and Citrus sinensis,indicating that they belong to the same evolutionary branch.

Key words: Citrus grandis var. Shatinyu Hort, S-RNase gene, sequence analysis

CLC Number: 

  • Q946
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