Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (4): 84-90.

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Optimization of Extraction Process of Alkaloids from Meconopsis horridula Hook. f. & Thomson by Response Surface Methodology

LUO Yun-yan1,2, LI Rong-zheng3, LI Bing4, DING Chen-xu5   

  1. 1.College of Life Science and Chemistry, Qinghai University for Nationalities, Xining Qinghai 810007, China;
    2. Key Laboratory of Plant Resources and Chemistry of Qinghai-Tibet Plateau, Xining Qinghai 810007, China;
    3. Qinghai Red Cross Hospital, Xining Qinghai 810001, China;
    4. Science and Technology Information Institute of Qinghai, Xining Qinghai 810001, China;
    5. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining Qinghai 810001,China
  • Received:2014-06-19 Published:2018-09-26

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Abstract: Response surface method (RSM) is applied to optimize the extraction of alkaloids from Meconopsis horridula Hook. f. & Thomson. Based on single experiment, hydrochloric acid concentration, liquid-solid ratio and extracting temperature are selected for designing, the extraction yield of alkaloids as response value. Box-Benhnken central combination experiment is used to set a mathematical model for response surface analysis. The results of RSM show that the optimal extraction conditions are: hydrochloric acid concentration is 3.7%, liquid-solid ratio is 38∶1, and extracting temperature is 56 ℃. Under these conditions, the extraction yield of alkaloids is up to 1.18%.

Key words: RSM, Meconopsis horridula Hook. f. &, Thomson, alkaloids, extraction

CLC Number: 

  • Q946.88
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