Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (2): 175-180.

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Molecular Cloning and Expression Analysis of Slow Myosin Heavy Chain 1 (sMyHC1) Gene cDNA in Mandarin Fish(Siniperca chuatsi)

YI Tan1,2 , LIU Xi-liang2,3, BIN Shi-yu3, WANG Kai-zhuo2, WU Ping2, CHU Wu-ying2, CHEN Tao1   

  1. 1.College of Veterinary Medicine,Hunan Agricultural University, Changsha Hunan 410128,China;
    2. College of Biological Engineering and Environmental Science, Changsha University,Changsha Hunan 410003,China;
    3.College of Life Science, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2014-03-10 Online:2014-06-25 Published:2018-09-25

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Abstract: The slow Myosin heavy chain 1 of Mandarin fish was cloned by Homologous cloning technology. The slow Myosin heavy chain 1 gene is 5 823 bp in length and encodes 1 940 amino acids. Sequence analysis showed that the slow Myosin heavy chain 1 contains three ATP binding sites,three actin binding sites, one ELC binding site,one RLC binding site and two Loops ring. The expression level in red muscle was significantly higher than that in white muscle and cardiac muscle. The above results will make contribution to provide reliable experimental data and theoretical basis for the muscle phenotype and the research of meat quility of Siniperca chuatsi.

Key words: Siniperca chuatsi, red muscle, myosin heavy chain gene, gene clone, sequence analysis

CLC Number: 

  • 753
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